PMID- 34060448
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 37
IP  - 6
DP  - 2021 Jun
TI  - [IL-6, IL-1beta, and IL-10 levels in peripheral blood as indicators for early 
      identification of gram-positive and gram-negative sepsis].
PG  - 532-537
AB  - Objective To find out indicators for rapid identification of Gram-positive (G(+)) 
      and Gram-negative (G(-)) bacteria through transcriptome sequencing of peripheral 
      blood mononuclear cells (PBMCs) and serum liquid-phase chip technology in early 
      sepsis. Methods 35 eligible cases out of 182 sepsis patients in the emergency 
      intensive care unit (EICU) were selected for retrospective analysis. They were 
      divided into G(+) group (12 cases) and G(-) group (23 cases) based on their blood 
      culture results. General characteristics like patients' age, gender, sequential 
      organ failure assessment (SOFA) scores, etc. and other laboratory indexes such as 
      blood routine, IL-6, CRP, procalcitonin(PCT)of these two groups were analyzed. 
      PBMCs were isolated through single density gradient centrifugation. Total RNA was 
      extracted for transcriptome sequencing to find out differential genes. Serum 
      liquid-phase chip technology was performed to detect serum granulocyte colony 
      stimulating factor (G-CSF), granulocyte-macrophage colony stimulating factor 
      (GM-CSF), IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, 
      IL-17, monocytes chemotactic protein 1 (MCP-1), tumor necrosis factor alpha 
      (TNF-alpha) in two groups. Receiver operating characteristic (ROC) curve was drawn 
      using bacteria types as a dependent variable and selected cytokines as test 
      variables, to analyze the correlation between selected biomarkers and bacteria 
      type. Results No significant difference in general characteristics, CRP, and PCT 
      were found between the G(+) and G(-) group. The serum level of IL-6 in G(+) group 
      was lower than that in the G(-) group. Transcriptome sequencing results revealed 
      30 immune-related genes that were differentially expressed in the PBMCs of two 
      groups. Compared to the G(+) group, the serum levels of IL-6 and IL-1beta in G(-) 
      group significantly increased, while serum IL-10 was reduced. ROC curve analysis 
      indicated that serum IL-6, IL-1beta, and IL-10 levels could identify the G(-) and 
      G(+) bacteria types. The combined diagnosis using these three indicators is 
      highly applicable in distinguishing G(-) and G(+) bacteria. Conclusion IL-6, 
      IL-1beta and IL-10 levels can be used as indicators for early identification of 
      sepsis induced by G(+) or G(-) bacteria.
FAU - Hu, Shanbo
AU  - Hu S
AD  - Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 
      710032, China.
FAU - Yang, Jing
AU  - Yang J
AD  - Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 
      710032, China.
FAU - Yu, Chaoping
AU  - Yu C
AD  - Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 
      710032, China.
FAU - Feng, Zhusheng
AU  - Feng Z
AD  - Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 
      710032, China.
FAU - Zhang, Jinxin
AU  - Zhang J
AD  - Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 
      710032, China.
FAU - Yin, Wen
AU  - Yin W
AD  - Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 
      710032, China. *Corresponding authors, E-mail: xjyyyw@126.com.
FAU - Xie, Jiangang
AU  - Xie J
AD  - Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an 
      710032, China. *Corresponding authors, E-mail: william@fmmu.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Interleukin-6)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Humans
MH  - *Interleukin-10
MH  - Interleukin-6/genetics
MH  - Leukocytes, Mononuclear
MH  - Retrospective Studies
MH  - *Sepsis/diagnosis
EDAT- 2021/06/02 06:00
MHDA- 2021/06/03 06:00
CRDT- 2021/06/01 08:43
PHST- 2021/06/01 08:43 [entrez]
PHST- 2021/06/02 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2021 Jun;37(6):532-537.