PMID- 34061343
OWN - NLM
STAT- MEDLINE
DCOM- 20211116
LR  - 20211116
IS  - 1976-3794 (Electronic)
IS  - 1225-8873 (Linking)
VI  - 59
IP  - 8
DP  - 2021 Aug
TI  - NF-kappaB/ROS and ERK pathways regulate NLRP3 inflammasome activation in Listeria 
      monocytogenes infected BV2 microglia cells.
PG  - 771-781
LID - 10.1007/s12275-021-0692-9 [doi]
AB  - Listeria monocytogenes is a food-borne pathogen responsible for neurolisteriosis, 
      which is potentially lethal in immunocompromised individuals. Microglia are the 
      main target cells for L. monocytogenes in central nervous system (CNS). However, 
      the precise mechanisms by which they trigger neuroinflammatory processes remain 
      unknown. The BV2 microglial cell line and a murine model of L. monocytogenes 
      infection were used for experiments in this study. Listeria monocytogenes induced 
      pyroptosis and nucleotide binding and oligomerization, leucine-rich repeat, pyrin 
      domain-containing 3 (NLRP3) inflammasome activation in BV2. Pharmacological 
      inhibition of the NLRP3 inflammasome attenuated L. monocytogenes-induced 
      pyroptosis. Moreover, inhibition of nuclear factor kappa-B (NF-kappaB) and 
      extracellular regulated protein kinases (ERK) pathways induced a decrease in 
      caspase1 activation and mature IL-1beta-17 secretion. Our collective findings 
      support critical involvement of the NLRP3 inflammasome in L. 
      monocytogenes-induced neuroinflammation and, to an extent, ROS production. In 
      addition, ERK and NF-kappaB signaling play an important role in activation of the 
      NLRP3 inflammasome, both in vitro and in vivo.
CI  - (c) 2021. The Microbiological Society of Korea.
FAU - Yuan, Lin
AU  - Yuan L
AD  - School of Medicine, Jiangsu University, Zhenjiang, 212013, P. R. China.
AD  - Department of Clinical Laboratory, Northern Jiangsu People's Hospital, Yangzhou, 
      225001, P. R. China.
FAU - Zhu, Yurong
AU  - Zhu Y
AD  - School of Medicine, Jiangsu University, Zhenjiang, 212013, P. R. China.
AD  - Department of Microbiology Laboratory, Linfen Central Hospital, Linfen, 041000, 
      P. R. China.
FAU - Huang, Shuang
AU  - Huang S
AD  - School of Medicine, Jiangsu University, Zhenjiang, 212013, P. R. China.
FAU - Lin, Lin
AU  - Lin L
AD  - School of Medicine, Jiangsu University, Zhenjiang, 212013, P. R. China.
FAU - Jiang, Xugan
AU  - Jiang X
AD  - School of Medicine, Jiangsu University, Zhenjiang, 212013, P. R. China.
FAU - Chen, Shengxia
AU  - Chen S
AD  - School of Medicine, Jiangsu University, Zhenjiang, 212013, P. R. China. 
      chensxia@ujs.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210601
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (Inflammasomes)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Inflammasomes/genetics/*immunology
MH  - Listeria monocytogenes/genetics/*physiology
MH  - Listeriosis/genetics/*immunology/microbiology/physiopathology
MH  - MAP Kinase Signaling System
MH  - Mice
MH  - Microglia/cytology/immunology/*microbiology
MH  - NF-kappa B/genetics/*immunology
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/genetics/*immunology
MH  - Pyroptosis
MH  - Reactive Oxygen Species/*immunology
MH  - Signal Transduction
OTO - NOTNLM
OT  - Listeria monocytogenes
OT  - leucine-rich repeat
OT  - microglia
OT  - mitogen-activated protein kinase (MAPK)
OT  - nuclear factor kappa-B (NF-kappaB)
OT  - nucleotide binding and oligomerization
OT  - pyrin domain-containing 3 (NLRP3) inflammasome
OT  - pyroptosis
OT  - reactive oxygen species (ROS)
EDAT- 2021/06/02 06:00
MHDA- 2021/11/17 06:00
CRDT- 2021/06/01 12:28
PHST- 2020/12/28 00:00 [received]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/04/12 00:00 [revised]
PHST- 2021/06/02 06:00 [pubmed]
PHST- 2021/11/17 06:00 [medline]
PHST- 2021/06/01 12:28 [entrez]
AID - 10.1007/s12275-021-0692-9 [pii]
AID - 10.1007/s12275-021-0692-9 [doi]
PST - ppublish
SO  - J Microbiol. 2021 Aug;59(8):771-781. doi: 10.1007/s12275-021-0692-9. Epub 2021 
      Jun 1.