PMID- 34062160
OWN - NLM
STAT- MEDLINE
DCOM- 20210721
LR  - 20210721
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 182
DP  - 2021 Jul 1
TI  - Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel 
      based on chemical/physical microenvironment.
PG  - 1941-1952
LID - S0141-8130(21)01171-5 [pii]
LID - 10.1016/j.ijbiomac.2021.05.188 [doi]
AB  - Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells 
      (BMSCs) successfully is crucial for cartilage regeneration. To date, various 
      hydrogels with different chemical microenvironment have been used to modulate 
      chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid 
      hydrogel. However, the chondro-inductive ability of collagen and hyaluronic acid 
      hydrogel has not been evaluated yet and the different chemical and physical 
      microenvironment of these two hydrogels increase the difficulty of comparison. In 
      this study, three different hydrogels based on collagen and hyaluronic acid 
      (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel 
      cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) 
      were prepared and their chondro-inductive ability on the encapsulated BMSCs was 
      evaluated. Col and Cgp have the same chemical composition and similar 
      microstructure, but are different from HA, while Cgp and HA hydrogels have the 
      same mechanical strength. It was found that chemical and physical 
      microenvironments of the hydrogels combined to influence cell condensation. 
      Thanks to cell condensation was more likely to occur in collagen hydrogels in the 
      early stage, the cartilage-induced ability was in the order of Col > Cgp > HA. 
      However, the severe shrinkage of Col and Cgp resulted in no enough space for cell 
      proliferation within hydrogels in the later stage. In contrast, relatively stable 
      physical microenvironment of HA helped to maintain continuous production of 
      cartilage-related matrix in the later stage. Overall, these results revealed that 
      the chondro-inductive ability of collagen and hyaluronic acid hydrogel with 
      different chemical and physical microenvironment cannot be evaluated by a 
      particular time period. However, it provided important information for 
      optimization and design of the future hydrogels towards successful repair of 
      articular cartilage.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Yang, Jirong
AU  - Yang J
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 61004, Sichuan, China; Research Center for Human Tissue and Organs 
      Degeneration, Institute Biomedical and Biotechnology, Shenzhen Institute of 
      Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangzhou, China.
FAU - Tang, Zizhao
AU  - Tang Z
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 61004, Sichuan, China.
FAU - Liu, Yifan
AU  - Liu Y
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 61004, Sichuan, China.
FAU - Luo, Zhaocong
AU  - Luo Z
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 61004, Sichuan, China.
FAU - Xiao, Yumei
AU  - Xiao Y
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 61004, Sichuan, China. Electronic address: xymzl2000@126.com.
FAU - Zhang, Xingdong
AU  - Zhang X
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 61004, Sichuan, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20210529
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Blood Proteins)
RN  - 0 (Hydrogels)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Blood Proteins/metabolism
MH  - Cattle
MH  - Cell Differentiation/drug effects
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - Cellular Microenvironment/*drug effects
MH  - Chondrocytes/*cytology/drug effects
MH  - Chondrogenesis/drug effects
MH  - Collagen/*pharmacology
MH  - Diffusion
MH  - Extracellular Matrix/drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Hyaluronic Acid/*pharmacology
MH  - Hydrogels/*pharmacology
MH  - Mesenchymal Stem Cells/cytology/drug effects/metabolism
MH  - Rabbits
MH  - Static Electricity
MH  - Transforming Growth Factor beta1/metabolism
OTO - NOTNLM
OT  - Chondrogenic differentiation
OT  - Hydrogels
OT  - Physical/chemical microenvironment
EDAT- 2021/06/02 06:00
MHDA- 2021/07/22 06:00
CRDT- 2021/06/01 20:10
PHST- 2021/03/28 00:00 [received]
PHST- 2021/05/14 00:00 [revised]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/06/02 06:00 [pubmed]
PHST- 2021/07/22 06:00 [medline]
PHST- 2021/06/01 20:10 [entrez]
AID - S0141-8130(21)01171-5 [pii]
AID - 10.1016/j.ijbiomac.2021.05.188 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2021 Jul 1;182:1941-1952. doi: 
      10.1016/j.ijbiomac.2021.05.188. Epub 2021 May 29.