PMID- 34064575
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210605
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 14
IP  - 10
DP  - 2021 May 11
TI  - Whey as an Alternative Nutrient Medium for Growth of Sporosarcina pasteurii and 
      Its Effect on CaCO(3) Polymorphism and Fly Ash Bioconsolidation.
LID - 10.3390/ma14102470 [doi]
LID - 2470
AB  - Whey in large quantities can cause environmental problems when discarded, because 
      it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an 
      easily available and economical alternative to reduce culture medium costs in 
      microbially induced calcium carbonate precipitation (MICP). In this work, a 
      native Sporosarcina pasteurii was isolated and then cultured by using different 
      proportions of whey (W) in nutrient broth (NB). The solids were characterized by 
      XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were 
      also studied. Whey concentration was directly related to CaCO(3) production. 
      Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All 
      experiments showed calcite and vaterite fractions, where a whey increment in the 
      media increased calcite content and decreased vaterite content, causing a 
      decrease in crystal size. MICP improved compressive strength (CS) in sand and fly 
      ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 
      kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in 
      biogenic CaCO(3) production. Material bioconsolidation depends on the CaCO(3) 
      polymorph, thus fly ash was effectively bioconsolidated by crystallization of 
      vaterite and sand by crystallization of calcite.
FAU - Chaparro, Sandra
AU  - Chaparro S
AD  - School of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological 
      University of Colombia, 150001 Tunja, Colombia.
FAU - Rojas, Hugo A
AU  - Rojas HA
AUID- ORCID: 0000-0003-3906-4522
AD  - School of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological 
      University of Colombia, 150001 Tunja, Colombia.
FAU - Caicedo, Gerardo
AU  - Caicedo G
AUID- ORCID: 0000-0003-2320-0980
AD  - School of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological 
      University of Colombia, 150001 Tunja, Colombia.
FAU - Romanelli, Gustavo
AU  - Romanelli G
AD  - Research and Development Centre on Applied Sciences "Dr. Jorge Ronco" (CCT-La 
      Plata-CONICET, CIC-PBA), National University of La Plata, 1900 La Plata, 
      Argentina.
FAU - Pineda, Antonio
AU  - Pineda A
AD  - Departamento de Quimica Organica, Universidad de Cordoba, Ctra NNal IV-A, Km 396, 
      E-14014 Cordoba, Spain.
AD  - Scientific Center for Molecular Design and Synthesis of Innovative Compounds for 
      the Medical Industry, Peoples Friendship University of Russia (RUDN University), 
      117198 Moscow, Russia.
FAU - Luque, Rafael
AU  - Luque R
AD  - Departamento de Quimica Organica, Universidad de Cordoba, Ctra NNal IV-A, Km 396, 
      E-14014 Cordoba, Spain.
AD  - Scientific Center for Molecular Design and Synthesis of Innovative Compounds for 
      the Medical Industry, Peoples Friendship University of Russia (RUDN University), 
      117198 Moscow, Russia.
FAU - Martinez, Jose J
AU  - Martinez JJ
AUID- ORCID: 0000-0002-4906-7121
AD  - School of Chemical Sciences, Faculty of Sciences, Pedagogical and Technological 
      University of Colombia, 150001 Tunja, Colombia.
LA  - eng
PT  - Journal Article
DEP - 20210511
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC8151748
OTO - NOTNLM
OT  - biogenic CaCO3
OT  - polymorphism
OT  - ureolytic bacteria
OT  - whey
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2021/06/03 06:00
MHDA- 2021/06/03 06:01
PMCR- 2021/05/11
CRDT- 2021/06/02 01:10
PHST- 2021/04/07 00:00 [received]
PHST- 2021/04/30 00:00 [revised]
PHST- 2021/05/05 00:00 [accepted]
PHST- 2021/06/02 01:10 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/06/03 06:01 [medline]
PHST- 2021/05/11 00:00 [pmc-release]
AID - ma14102470 [pii]
AID - materials-14-02470 [pii]
AID - 10.3390/ma14102470 [doi]
PST - epublish
SO  - Materials (Basel). 2021 May 11;14(10):2470. doi: 10.3390/ma14102470.