PMID- 34065092
OWN - NLM
STAT- MEDLINE
DCOM- 20210830
LR  - 20210830
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 12
IP  - 5
DP  - 2021 May 9
TI  - Transcriptomic Changes in Mouse Bone Marrow-Derived Macrophages Exposed to 
      Neuropeptide FF.
LID - 10.3390/genes12050705 [doi]
LID - 705
AB  - Neuropeptide FF (NPFF) is a neuropeptide that regulates various biological 
      activities. Currently, the regulation of NPFF on the immune system is an emerging 
      field. However, the influence of NPFF on the transcriptome of primary macrophages 
      has not been fully elucidated. In this study, the effect of NPFF on the 
      transcriptome of mouse bone marrow-derived macrophages (BMDMs) was explored by 
      RNA sequencing, bioinformatics, and molecular simulation. BMDMs were treated with 
      1 nM NPFF for 18 h, followed by RNA sequencing. Differentially expressed genes 
      (DEGs) were obtained, followed by GO, KEGG, and PPI analysis. A total of eight 
      qPCR-validated DEGs were selected as hub genes. Subsequently, the 
      three-dimensional (3-D) structures of the eight hub proteins were constructed by 
      Modeller and Rosetta. Next, the molecular dynamics (MD)-optimized 3-D structure 
      of hub protein was acquired with Gromacs. Finally, the binding modes between NPFF 
      and hub proteins were studied by Rosetta. A total of 2655 DEGs were obtained 
      (up-regulated 1442 vs. down-regulated 1213), and enrichment analysis showed that 
      NPFF extensively regulates multiple functional pathways mediated by BMDMs. 
      Moreover, the 3-D structure of the hub protein was obtained after 
      MD-optimization. Finally, the docking modes of NPFF-hub proteins were predicted. 
      Besides, NPFFR2 was expressed on the cell membrane of BMDMs, and NPFF 1 nM 
      significantly activated NPFFR2 protein expression. In summary, instead of 
      significantly inhibiting the expression of the immune-related gene transcriptome 
      of RAW 264.7 cells, NPFF simultaneously up-regulated and down-regulated the gene 
      expression profile of a large number of BMDMs, hinting that NPFF may profoundly 
      affect a variety of cellular processes dominated by BMDMs. Our work provides 
      transcriptomics clues for exploring the influence of NPFF on the physiological 
      functions of BMDMs.
FAU - Sun, Yulong
AU  - Sun Y
AUID- ORCID: 0000-0002-6811-5926
AD  - School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, 
      China.
AD  - Key Laboratory for Space Biosciences & Biotechnology, Institute of Special 
      Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical 
      University, Xi'an 710072, China.
FAU - Kuang, Yuanyuan
AU  - Kuang Y
AD  - School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, 
      China.
AD  - Key Laboratory for Space Biosciences & Biotechnology, Institute of Special 
      Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical 
      University, Xi'an 710072, China.
FAU - Zuo, Zhuo
AU  - Zuo Z
AD  - School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, 
      China.
AD  - Key Laboratory for Space Biosciences & Biotechnology, Institute of Special 
      Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical 
      University, Xi'an 710072, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210509
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (Oligopeptides)
RN  - 0 (Receptors, Neuropeptide)
RN  - 0 (neuropeptide FF receptor)
RN  - 99566-27-5 
      (phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Cells, Cultured
MH  - Macrophages/drug effects/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oligopeptides/*metabolism/pharmacology
MH  - Protein Binding
MH  - Receptors, Neuropeptide/chemistry/genetics/metabolism
MH  - *Transcriptome
PMC - PMC8151073
OTO - NOTNLM
OT  - RNA sequencing
OT  - bioinformatics
OT  - bone marrow-derived macrophage
OT  - neuropeptide
OT  - transcriptomic profiles
COIS- The authors declare that there is no conflict of interest.
EDAT- 2021/06/03 06:00
MHDA- 2021/08/31 06:00
PMCR- 2021/05/09
CRDT- 2021/06/02 01:11
PHST- 2021/03/05 00:00 [received]
PHST- 2021/04/28 00:00 [revised]
PHST- 2021/05/04 00:00 [accepted]
PHST- 2021/06/02 01:11 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/08/31 06:00 [medline]
PHST- 2021/05/09 00:00 [pmc-release]
AID - genes12050705 [pii]
AID - genes-12-00705 [pii]
AID - 10.3390/genes12050705 [doi]
PST - epublish
SO  - Genes (Basel). 2021 May 9;12(5):705. doi: 10.3390/genes12050705.