PMID- 34065697
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210615
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 10
IP  - 5
DP  - 2021 May 16
TI  - Quercetin and Isorhamnetin Attenuate Benzo[a]pyrene-Induced Toxicity by 
      Modulating Detoxification Enzymes through the AhR and NRF2 Signaling Pathways.
LID - 10.3390/antiox10050787 [doi]
LID - 787
AB  - Benzo[a]pyrene, classified as a Group 1 carcinogen, is metabolized to 
      B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), causing DNA mutations and eventually 
      cancer. Quercetin is a dietary flavonoid abundant in fruits and vegetables. After 
      quercetin intake, quercetin's metabolites isorhamnetin and miquelianin are more 
      highly concentrated than quercetin in the human plasma. In this study, we 
      investigated the molecular mechanisms associated with the cytoprotective effect 
      of quercetin and its metabolites against benzo[a]pyrene from a detoxification 
      perspective. Quercetin and its metabolite isorhamnetin reduced 
      benzo[a]pyrene-induced cytotoxicity, whereas the metabolite miquelianin did not 
      mitigate benzo[a]pyrene-induced cytotoxicity. Moreover, quercetin and 
      isorhamnetin reduced intracellular levels of BPDE-DNA adducts. The formation and 
      elimination of BPDE is mediated by the xenobiotic detoxification process. 
      Quercetin and isorhamnetin increased the gene and protein expression levels of 
      phase I, II, and III enzymes involved in xenobiotic detoxification. Furthermore, 
      quercetin and isorhamnetin induced the translocation of aryl hydrocarbon receptor 
      (AhR) and nuclear factor erythroid 2-related factor 2 (NRF2), which regulate the 
      expression level of phase enzymes. Our results suggest that quercetin and 
      isorhamnetin promote the metabolism, detoxification, and elimination of B[a]P, 
      thereby increasing anti-genotoxic effects and protecting against B[a]P-induced 
      cytotoxicity.
FAU - Kim, Min
AU  - Kim M
AUID- ORCID: 0000-0001-7287-7002
AD  - Department of Life Science, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang 10326, Gyeonggi-do, Korea.
FAU - Jee, Seung-Cheol
AU  - Jee SC
AUID- ORCID: 0000-0002-9635-9362
AD  - Department of Life Science, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang 10326, Gyeonggi-do, Korea.
FAU - Kim, Kyeong-Seok
AU  - Kim KS
AD  - Division of Toxicology, School of Pharmacy, Sungkyunkwan University-Suwon, Suwon 
      16419, Gyeonggi-do, Korea.
FAU - Kim, Hyung-Sik
AU  - Kim HS
AUID- ORCID: 0000-0001-7657-3970
AD  - Division of Toxicology, School of Pharmacy, Sungkyunkwan University-Suwon, Suwon 
      16419, Gyeonggi-do, Korea.
FAU - Yu, Kyoung-Nae
AU  - Yu KN
AD  - Department of Life Science, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang 10326, Gyeonggi-do, Korea.
FAU - Sung, Jung-Suk
AU  - Sung JS
AD  - Department of Life Science, Dongguk University-Seoul, Biomedi Campus, 32 
      Dongguk-ro, Ilsandong-gu, Goyang 10326, Gyeonggi-do, Korea.
LA  - eng
GR  - 14162KFDA072/Korea Food and Drug Administration/
GR  - 2017001970003/Ministry of Environment of korea/
PT  - Journal Article
DEP - 20210516
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC8156367
OTO - NOTNLM
OT  - AhR
OT  - NRF2
OT  - benzo[a]pyrene
OT  - isorhamnetin
OT  - quercetin
OT  - xenobiotic metabolism
COIS- The authors declare no conflict of interest.
EDAT- 2021/06/03 06:00
MHDA- 2021/06/03 06:01
PMCR- 2021/05/16
CRDT- 2021/06/02 01:13
PHST- 2021/04/26 00:00 [received]
PHST- 2021/05/13 00:00 [revised]
PHST- 2021/05/14 00:00 [accepted]
PHST- 2021/06/02 01:13 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/06/03 06:01 [medline]
PHST- 2021/05/16 00:00 [pmc-release]
AID - antiox10050787 [pii]
AID - antioxidants-10-00787 [pii]
AID - 10.3390/antiox10050787 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2021 May 16;10(5):787. doi: 10.3390/antiox10050787.