PMID- 34066973
OWN - NLM
STAT- MEDLINE
DCOM- 20210616
LR  - 20240226
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 9
DP  - 2021 May 7
TI  - Methionine Diet Evoked Hyperhomocysteinemia Causes Hippocampal Alterations, 
      Metabolomics Plasma Changes and Behavioral Pattern in Wild Type Rats.
LID - 10.3390/ijms22094961 [doi]
LID - 4961
AB  - L-methionine, an essential amino acid, plays a critical role in cell physiology. 
      High intake and/or dysregulation in methionine (Met) metabolism results in 
      accumulation of its intermediate(s) or breakdown products in plasma, including 
      homocysteine (Hcy). High level of Hcy in plasma, hyperhomocysteinemia (hHcy), is 
      considered to be an independent risk factor for cerebrovascular diseases, stroke 
      and dementias. To evoke a mild hHcy in adult male Wistar rats we used an enriched 
      Met diet at a dose of 2 g/kg of animal weight/day in duration of 4 weeks. The 
      study contributes to the exploration of the impact of Met enriched diet inducing 
      mild hHcy on nervous tissue by detecting the histo-morphological, metabolomic and 
      behavioural alterations. We found an altered plasma metabolomic profile, modified 
      spatial and learning memory acquisition as well as remarkable histo-morphological 
      changes such as a decrease in neurons' vitality, alterations in the morphology of 
      neurons in the selective vulnerable hippocampal CA 1 area of animals treated with 
      Met enriched diet. Results of these approaches suggest that the mild hHcy alters 
      plasma metabolome and behavioural and histo-morphological patterns in rats, 
      likely due to the potential Met induced changes in "methylation index" of 
      hippocampal brain area, which eventually aggravates the noxious effect of high 
      methionine intake.
FAU - Kovalska, Maria
AU  - Kovalska M
AUID- ORCID: 0000-0001-6802-5309
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Baranovicova, Eva
AU  - Baranovicova E
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Kalenska, Dagmar
AU  - Kalenska D
AD  - Department of Anatomy, Jessenius Faculty of Medicine, Comenius University in 
      Bratislava, 03601 Martin, Slovakia.
FAU - Tomascova, Anna
AU  - Tomascova A
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Adamkov, Marian
AU  - Adamkov M
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Kovalska, Libusa
AU  - Kovalska L
AD  - Clinic of Stomatology and Maxillofacial Surgery, Jessenius Faculty of Medicine, 
      Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Lehotsky, Jan
AU  - Lehotsky J
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
LA  - eng
GR  - 230/20/Vedecka Grantova Agentura MSVVaS SR a SAV/
GR  - 15/107/Agentura na Podporu Vyskumu a Vyvoja/
PT  - Journal Article
DEP - 20210507
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - *Behavior, Animal
MH  - Hippocampus/*pathology
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/*blood/*metabolism/pathology
MH  - In Situ Nick-End Labeling
MH  - Magnetic Resonance Spectroscopy
MH  - Male
MH  - *Metabolomics
MH  - Methionine
MH  - Rats, Wistar
MH  - Staining and Labeling
MH  - Rats
PMC - PMC8124831
OTO - NOTNLM
OT  - hyperhomocysteinemia
OT  - methionine diet
OT  - morris water maze
OT  - neurodegeneration
OT  - wild-type rats
COIS- The authors declare no conflict of interest.
EDAT- 2021/06/03 06:00
MHDA- 2021/06/17 06:00
PMCR- 2021/05/07
CRDT- 2021/06/02 01:17
PHST- 2021/03/18 00:00 [received]
PHST- 2021/04/12 00:00 [revised]
PHST- 2021/05/03 00:00 [accepted]
PHST- 2021/06/02 01:17 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/06/17 06:00 [medline]
PHST- 2021/05/07 00:00 [pmc-release]
AID - ijms22094961 [pii]
AID - ijms-22-04961 [pii]
AID - 10.3390/ijms22094961 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 May 7;22(9):4961. doi: 10.3390/ijms22094961.