PMID- 34068086
OWN - NLM
STAT- MEDLINE
DCOM- 20210624
LR  - 20210624
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 10
DP  - 2021 May 13
TI  - Optimization of an Ultrasound-Assisted Extraction Method for the Analysis of 
      Major Anthocyanin Content in Erica australis Flowers.
LID - 10.3390/molecules26102884 [doi]
LID - 2884
AB  - Erica australis plants have been used in infusions and folk medicine for years 
      for its diuretic and antiseptic properties and even for the treatment of 
      infections. In addition, a recently published thorough study on this species has 
      demonstrated its antioxidant, antibiotic, anti-inflammatory, anticarcinogenic and 
      even antitumoral activities. These properties have been associated with the high 
      content of anthocyanins in E. australis leaves and flowers. The aim of the 
      present research is to optimize an ultrasound-assisted extraction methodology for 
      the recovery of the anthocyanins present in E. australis flowers. For that 
      purpose, a Box Behnken design with response surface methodology was employed, and 
      the influence of four variables at different values was determined: namely, the 
      composition of the extraction solvents (0-50% MeOH in water), the pH level of 
      those solvents (3-7), the extraction temperature (10-70  degrees C), and the 
      sample:solvent ratio (0.5 g:10 mL-0.5 g:20 mL). UHPLC-UV-vis has been employed to 
      quantify the two major anthocyanins detected in the samples. The extraction 
      optimum conditions for 0.5 g samples were: 20 mL of solvent (50% MeOH:H(2)O) at 5 
      pH, with a 15 min extraction time at 70  degrees C. A precision study was performed and 
      the intra-day and inter-day relative standard deviations (RSDs) obtained were 
      3.31% and 3.52%, respectively. The developed methodology has been successfully 
      applied to other Erica species to validate the suitability of the method for 
      anthocyanin extraction.
FAU - Carrera, Ceferino
AU  - Carrera C
AUID- ORCID: 0000-0002-6835-8303
AD  - Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of 
      International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 
      Cadiz, Spain.
FAU - Aliano-Gonzalez, Maria Jose
AU  - Aliano-Gonzalez MJ
AUID- ORCID: 0000-0002-5816-4476
AD  - Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of 
      International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 
      Cadiz, Spain.
FAU - Rodriguez-Lopez, Jaime
AU  - Rodriguez-Lopez J
AD  - Department of Biology, Faculty of Marine and Environmental Sciences, University 
      of Cadiz, Puerto Real, 11510 Cadiz, Spain.
FAU - Ferreiro-Gonzalez, Marta
AU  - Ferreiro-Gonzalez M
AUID- ORCID: 0000-0003-0870-403X
AD  - Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of 
      International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 
      Cadiz, Spain.
FAU - Ojeda-Copete, Fernando
AU  - Ojeda-Copete F
AUID- ORCID: 0000-0001-5480-0925
AD  - Department of Biology, Faculty of Marine and Environmental Sciences, University 
      of Cadiz, Puerto Real, 11510 Cadiz, Spain.
FAU - Barbero, Gerardo F
AU  - Barbero GF
AUID- ORCID: 0000-0001-7302-6605
AD  - Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of 
      International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 
      Cadiz, Spain.
FAU - Palma, Miguel
AU  - Palma M
AUID- ORCID: 0000-0001-8509-4226
AD  - Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of 
      International Excellence (ceiA3), IVAGRO, University of Cadiz, Puerto Real, 11510 
      Cadiz, Spain.
LA  - eng
GR  - EQC2018-005135-P/State Subprogram of Research Infrastructures and Technical 
      Scientific Equipment (Spain)/
PT  - Journal Article
DEP - 20210513
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Anthocyanins)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Anthocyanins/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Ericaceae/*chemistry
MH  - Flowers/*chemistry
MH  - Methanol/chemistry
MH  - Reference Standards
MH  - Temperature
MH  - Time Factors
MH  - Ultrasonics/*methods
PMC - PMC8152736
OTO - NOTNLM
OT  - Box-Behnken design
OT  - HPLC-UV-vis
OT  - Mediterranean heather
OT  - flower anthocyanins
OT  - optimization
OT  - ultrasound-assisted extraction
COIS- The authors declare no conflict of interest.
EDAT- 2021/06/03 06:00
MHDA- 2021/06/25 06:00
PMCR- 2021/05/13
CRDT- 2021/06/02 01:21
PHST- 2021/04/15 00:00 [received]
PHST- 2021/05/08 00:00 [revised]
PHST- 2021/05/11 00:00 [accepted]
PHST- 2021/06/02 01:21 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/06/25 06:00 [medline]
PHST- 2021/05/13 00:00 [pmc-release]
AID - molecules26102884 [pii]
AID - molecules-26-02884 [pii]
AID - 10.3390/molecules26102884 [doi]
PST - epublish
SO  - Molecules. 2021 May 13;26(10):2884. doi: 10.3390/molecules26102884.