PMID- 34069671
OWN - NLM
STAT- MEDLINE
DCOM- 20210702
LR  - 20210702
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 10
DP  - 2021 May 19
TI  - The CD200 Regulates Inflammation in Mice Independently of TNF-alpha Production.
LID - 10.3390/ijms22105358 [doi]
LID - 5358
AB  - Inflammatory bowel disease is characterized by the infiltration of immune cells 
      and chronic inflammation. The immune inhibitory receptor, CD200R, is involved in 
      the downregulation of the activation of immune cells to prevent excessive 
      inflammation. We aimed to define the role of CD200R ligand-CD200 in the 
      experimental model of intestinal inflammation in conventionally-reared mice. Mice 
      were given a dextran sodium sulfate solution in drinking water. Bodyweight loss 
      was monitored daily and the disease activity index was calculated, and a 
      histological evaluation of the colon was performed. TNF-alpha production was measured 
      in the culture of small fragments of the distal colon or bone marrow-derived 
      macrophages (BMDMs) cocultured with CD200(+) cells. We found that Cd200(-/-) mice 
      displayed diminished severity of colitis when compared to WT mice. Inflammation 
      significantly diminished CD200 expression in WT mice, particularly on vascular 
      endothelial cells and immune cells. The co-culture of BMDMs with CD200(+) cells 
      inhibited TNF-alpha secretion. In vivo, acute colitis induced by DSS significantly 
      increased TNF-alpha secretion in colon tissue in comparison to untreated controls. 
      However, Cd200(-/-) mice secreted a similar level of TNF-alpha to WT mice in vivo. 
      CD200 regulates the severity of DSS-induced colitis in conventionally-reared 
      mice. The presence of CD200(+) cells decreases TNF-alpha production by macrophages in 
      vitro. However, during DDS-induced intestinal inflammation secretion of TNF-alpha is 
      independent of CD200 expression.
FAU - Tonecka, Katarzyna
AU  - Tonecka K
AD  - Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland.
FAU - Braniewska, Agata
AU  - Braniewska A
AD  - Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland.
FAU - Pilch, Zofia
AU  - Pilch Z
AD  - Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland.
FAU - Sas, Zuzanna
AU  - Sas Z
AD  - Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland.
FAU - Skorzynski, Marcin
AU  - Skorzynski M
AD  - Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland.
FAU - Manuali, Elisabetta
AU  - Manuali E
AUID- ORCID: 0000-0003-3680-0926
AD  - Laboratory of Veterinary and Comparative Histopathology, Istituto Zooprofilattico 
      Sperimentale Umbria e Marche "Togo Rosati", 06126 Perugia, Italy.
FAU - Rygiel, Tomasz P
AU  - Rygiel TP
AUID- ORCID: 0000-0003-4746-6505
AD  - Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland.
LA  - eng
GR  - HOMING PLUS/2011-4/10/Fundacja na rzecz Nauki Polskiej/
GR  - 2014/15/B/NZ6/03716/Narodowe Centrum Nauki/
PT  - Journal Article
DEP - 20210519
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antigens, CD)
RN  - 0 (CD200 receptor, mouse)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - UQ4V77A8VA (antigens, CD200)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*genetics/metabolism
MH  - Colitis/pathology
MH  - Colon/pathology
MH  - Cytokines/metabolism
MH  - Endothelial Cells/metabolism
MH  - Female
MH  - Gene Expression/genetics
MH  - Gene Expression Regulation/genetics
MH  - Inflammation/genetics/*immunology
MH  - Inflammatory Bowel Diseases/metabolism/physiopathology
MH  - Macrophages/metabolism
MH  - Male
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Receptors, Immunologic/metabolism
MH  - Tumor Necrosis Factor-alpha/immunology/*metabolism
PMC - PMC8161250
OTO - NOTNLM
OT  - CD200
OT  - CD200R
OT  - immune regulation
OT  - inflammation
OT  - inflammatory bowel disease
OT  - myeloid cells
COIS- The authors declare no conflict of interest.
EDAT- 2021/06/03 06:00
MHDA- 2021/07/03 06:00
PMCR- 2021/05/19
CRDT- 2021/06/02 01:26
PHST- 2021/03/15 00:00 [received]
PHST- 2021/05/06 00:00 [revised]
PHST- 2021/05/14 00:00 [accepted]
PHST- 2021/06/02 01:26 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/07/03 06:00 [medline]
PHST- 2021/05/19 00:00 [pmc-release]
AID - ijms22105358 [pii]
AID - ijms-22-05358 [pii]
AID - 10.3390/ijms22105358 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 May 19;22(10):5358. doi: 10.3390/ijms22105358.