PMID- 34072497
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210714
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 9
IP  - 6
DP  - 2021 May 29
TI  - The Effects of Chemical and Mechanical Stresses on Bacillus cereus and 
      Pseudomonas fluorescens Single- and Dual-Species Biofilm Removal.
LID - 10.3390/microorganisms9061174 [doi]
LID - 1174
AB  - Biofilm control is mainly based on chemical disinfection, without a clear 
      understanding of the role of the biocides and process conditions on biofilm 
      removal. This study aims to understand the effects of a biocide 
      (benzyldimethyldodecyl ammonium chloride-BDMDAC) and mechanical treatment (an 
      increase of shear stress -tauw) on single- and dual-species biofilms formed by 
      Bacillus cereus and Pseudomonas fluorescens on high-density polyethene (HDPE). 
      BDMDAC effects were initially assessed on bacterial physicochemical properties 
      and initial adhesion ability. Then, mature biofilms were formed on a rotating 
      cylinder reactor (RCR) for 7 days to assess the effects of chemical and 
      mechanical treatments, and the combination of both on biofilm removal. The 
      results demonstrated that the initial adhesion does not predict the formation of 
      mature biofilms. It was observed that the dual-species biofilms were the most 
      susceptible to BDMDAC exposure. The exposure to increasing tauw emphasised the 
      mechanical stability of biofilms, as lower values of tauw (1.66 Pa) caused 
      high biofilm erosion and higher tauw values (17.7 Pa) seem to compress the 
      remaining biofilm. In general, the combination of BDMDAC and the mechanical 
      treatment was synergic in increasing biofilm removal. However, these were 
      insufficient to cause total biofilm removal (100%; an average standard deviation 
      of 11% for the method accuracy should be considered) from HDPE.
FAU - Gomes, Ines B
AU  - Gomes IB
AUID- ORCID: 0000-0002-0731-3662
AD  - LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of 
      Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
FAU - Lemos, Madalena
AU  - Lemos M
AD  - LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of 
      Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
FAU - Fernandes, Susana
AU  - Fernandes S
AUID- ORCID: 0000-0002-9132-5382
AD  - LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of 
      Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
FAU - Borges, Anabela
AU  - Borges A
AUID- ORCID: 0000-0001-6929-6805
AD  - LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of 
      Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
FAU - Simoes, Lucia C
AU  - Simoes LC
AD  - CEB-Centre of Biological Engineering, Campus de Gualtar, University of Minho, 
      4710-057 Braga, Portugal.
FAU - Simoes, Manuel
AU  - Simoes M
AUID- ORCID: 0000-0002-3355-4398
AD  - LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of 
      Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
LA  - eng
GR  - PTDC/BII-BTI/30219/2017 - POCI-01-0145-FEDER-030219/Fundacao para a Ciencia e a 
      Tecnologia/
GR  - POCI-01-0247-FEDER-072237/Agencia Nacional de Inovacao/
GR  - POCI-01-0247-FEDER-033298/Agencia Nacional de Inovacao/
PT  - Journal Article
DEP - 20210529
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC8228086
OTO - NOTNLM
OT  - BDMDAC
OT  - disinfection
OT  - high-density polyethene
OT  - rotating cylinder reactor
OT  - shear stress
COIS- The authors declare no conflict of interest.
EDAT- 2021/06/03 06:00
MHDA- 2021/06/03 06:01
PMCR- 2021/05/29
CRDT- 2021/06/02 01:35
PHST- 2021/04/22 00:00 [received]
PHST- 2021/05/22 00:00 [revised]
PHST- 2021/05/26 00:00 [accepted]
PHST- 2021/06/02 01:35 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/06/03 06:01 [medline]
PHST- 2021/05/29 00:00 [pmc-release]
AID - microorganisms9061174 [pii]
AID - microorganisms-09-01174 [pii]
AID - 10.3390/microorganisms9061174 [doi]
PST - epublish
SO  - Microorganisms. 2021 May 29;9(6):1174. doi: 10.3390/microorganisms9061174.