PMID- 34072898
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210615
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 10
IP  - 11
DP  - 2021 May 31
TI  - Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass 
      Spectrometry in Healthy Pregnant Women Living in Catalonia.
LID - 10.3390/jcm10112444 [doi]
LID - 2444
AB  - BACKGROUND: Recent guidelines recommend establishing a local reference interval 
      (RI) for thyroid function. We aimed to establish trimester-specific RIs for 
      thyrotropin (TSH) and free thyroxine (FT4) in a cohort of healthy pregnant women 
      in Catalonia (Spain). METHODS: A prospective observational study was conducted 
      with 332 healthy pregnant women, from the first trimester (1T) to delivery. TSH 
      was measured using an Architect((R)) immunoassay (Abbott) and FT4 by two 
      immunoassays, Architect((R)) (Abbott) and Cobas((R)) (Roche), in the three 
      trimesters. FT4 was also measured by liquid chromatography mass spectrometry 
      (LC/MS/MS) in the 1T. RESULTS: TSH (microUI/mL) increased throughout pregnancy (1T: 
      0.03-3.78; 2T: 0.51-3.53; 3T: 0.50-4.32; p < 0.0001) and FT4 (pmol/L) 
      progressively decreased (Architect((R)) 1T: 10.42-15.96; 2T: 8.37-12.74; 3T: 
      8.24-12.49; p < 0.0001; and Cobas((R)): 1T: 11.46-19.05; 2T: 9.65-14.67; 3T: 
      8.88-14.54; p < 0.0067). The FT4 RI during 1T determined LC/MS/MS was 8.75-18.27. 
      Despite the 1T FT4 results measured by LC/MS/MS and with the two immunoassays 
      being significantly correlated, the results obtained by the three methods were 
      found to be non-interchangeable. CONCLUSIONS: We established trimester-specific 
      RIs for TSH and for FT4 with immunoassays in our population. We also validated 
      the 1T FT4 using LC/MS/MS to confirm the results of FT4 lower than the 2.5th 
      percentile or higher than the 97.5th percentile.
FAU - Hernandez, Jose Maria
AU  - Hernandez JM
AUID- ORCID: 0000-0003-1363-067X
AD  - Institut d'Investigacio en Ciencies de la Salut Germans Trias i Pujol, s/n Cami 
      de les Escoles, 08916 Badalona, Spain.
FAU - Soldevila, Berta
AU  - Soldevila B
AD  - Endocrinology & Nutrition Department, Hospital Universitari Germans Trias i 
      Pujol, s/n Carretera del Canyet, 08916 Badalona, Spain.
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
FAU - Velasco, Ines
AU  - Velasco I
AUID- ORCID: 0000-0002-6143-6970
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
AD  - Gynecology & Obstetrics Department, Hospital Universitari Germans Trias i Pujol, 
      s/n Carretera del Canyet, 08916 Badalona, Spain.
AD  - Pediatrics, Gynecology & Obstetrics Department, Autonomous University of 
      Barcelona, Campus UAB, Placa Civica, 08193 Bellaterra, Spain.
FAU - Moreno-Flores, Fernando
AU  - Moreno-Flores F
AD  - Clinical Biochemistry Department, Hospital Universitari Germans Trias i Pujol, 
      s/n Carretera del Canyet, 08916 Badalona, Spain.
FAU - Ferrer, Laura
AU  - Ferrer L
AD  - Endocrinology & Nutrition Department, Hospital Universitari Germans Trias i 
      Pujol, s/n Carretera del Canyet, 08916 Badalona, Spain.
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
FAU - Perez-Montes de Oca, Alejandra
AU  - Perez-Montes de Oca A
AUID- ORCID: 0000-0001-8807-1935
AD  - Endocrinology & Nutrition Department, Hospital Universitari Germans Trias i 
      Pujol, s/n Carretera del Canyet, 08916 Badalona, Spain.
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
FAU - Santillan, Cecilia
AU  - Santillan C
AD  - Endocrinology Department, Doctor Arturo Onativia Hospital, 30 E.Paz Chain, Salta 
      A4400AWQ, Argentina.
FAU - Munoz, Carla
AU  - Munoz C
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
FAU - Ballesta, Silvia
AU  - Ballesta S
AD  - Endocrinology & Nutrition Department, Hospital Universitari Germans Trias i 
      Pujol, s/n Carretera del Canyet, 08916 Badalona, Spain.
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
FAU - Canal, Cristina
AU  - Canal C
AD  - Gynecology & Obstetrics Department, ASSIR La Riera, Hospital Universitari Germans 
      Trias i Pujol, 1 Riera Matamoros, 08911 Badalona, Spain.
FAU - Puig-Domingo, Manel
AU  - Puig-Domingo M
AD  - Endocrinology & Nutrition Department, Hospital Universitari Germans Trias i 
      Pujol, s/n Carretera del Canyet, 08916 Badalona, Spain.
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
FAU - Granada, Maria Luisa
AU  - Granada ML
AD  - Endocrine, Thyroid & Obesity Research Group, Institut d'Investigacio en Ciencies 
      de la Salut Germans Trias i Pujol, s/n Cami de les Escoles, 08916 Badalona, 
      Spain.
AD  - Clinical Biochemistry Department, Hospital Universitari Germans Trias i Pujol, 
      s/n Carretera del Canyet, 08916 Badalona, Spain.
LA  - eng
GR  - PI15/02192/Instituto de Salud Carlos III/
PT  - Journal Article
DEP - 20210531
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC8198941
OTO - NOTNLM
OT  - immunoassays
OT  - pregnancy
OT  - reference intervals
OT  - tandem mass
OT  - thyroid function
COIS- None of the authors have any conflicts of interest to declare.
EDAT- 2021/06/03 06:00
MHDA- 2021/06/03 06:01
PMCR- 2021/05/31
CRDT- 2021/06/02 01:36
PHST- 2021/05/09 00:00 [received]
PHST- 2021/05/24 00:00 [revised]
PHST- 2021/05/26 00:00 [accepted]
PHST- 2021/06/02 01:36 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/06/03 06:01 [medline]
PHST- 2021/05/31 00:00 [pmc-release]
AID - jcm10112444 [pii]
AID - jcm-10-02444 [pii]
AID - 10.3390/jcm10112444 [doi]
PST - epublish
SO  - J Clin Med. 2021 May 31;10(11):2444. doi: 10.3390/jcm10112444.