PMID- 34074384
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 232
DP  - 2021 Sep 1
TI  - High efficiency functionalized hydrophilic cyclofructans as stationary phases in 
      sub/supercritical fluid chromatography.
PG  - 122308
LID - S0039-9140(21)00229-0 [pii]
LID - 10.1016/j.talanta.2021.122308 [doi]
AB  - Packed column SFC has become very popular for preparative and analytical 
      separations due to the low cost of CO(2), its accessible critical temperature, 
      and pressure, with the additional benefit of a low environmental burden. 
      Currently, there is a shortage of new polar stationary phase chemistries for SFC. 
      In this work, two new functionalized cyclofructan columns are introduced and 
      evaluated for their performance in achiral SFC separations for the first time. 
      Cyclofructan (CF6), a macrocyclic oligosaccharide, was covalently linked with 
      benzoic acid (BCF6) and propyl sulfonic acid (SCF6) groups by ether bonds. 
      Superficially porous particles (2.7 mum) bonded with modified CF6 showed markedly 
      different selectivity than native CF6. In SFC, peak shapes of amines and basic 
      compounds are often compromised. We show that small quantities (~5.7% v/v) of 
      water added to the methanol modifier in CO(2) improves peak symmetries of 
      primary, secondary, and tertiary amines. Efficiencies as high as 200,000 plates/m 
      (reduced plate height ~ 1.8) were observed for benzamide and amitriptyline on the 
      BCF6 column. The relative standard deviations (RSDs) of retention times on BCF6 
      were about 1.4%, and on SCF6 were less than 1%. Amines on the SCF6 column showed 
      plate counts as high as 170,000 plates/m. Tetramethylammonium acetate is examined 
      as an alternative to water in MeOH. A run time of 36 min with methanol, 
      trifluoroacetic acid, triethylamine mobile phase was reduced to <5 min with 
      complete baseline resolution for a set of amines. The new stationary phases allow 
      greener approaches towards solving separation problems.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Firooz, Sepideh Khaki
AU  - Firooz SK
AD  - Department of Chemistry & Biochemistry, University of Texas at Arlington, TX, 
      76019, USA.
FAU - Wahab, M Farooq
AU  - Wahab MF
AD  - Department of Chemistry & Biochemistry, University of Texas at Arlington, TX, 
      76019, USA.
FAU - Yu, Jeongjae
AU  - Yu J
AD  - Department of Chemistry & Biochemistry, University of Texas at Arlington, TX, 
      76019, USA.
FAU - Armstrong, Daniel W
AU  - Armstrong DW
AD  - Department of Chemistry & Biochemistry, University of Texas at Arlington, TX, 
      76019, USA. Electronic address: sec4dwa@uta.edu.
LA  - eng
PT  - Journal Article
DEP - 20210318
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
SB  - IM
OTO - NOTNLM
OT  - Amines
OT  - Aqueous additives
OT  - Column efficiency
OT  - Derivatized cyclofructan
OT  - Drugs
OT  - Supercritical fluid chromatography
OT  - Superficially porous particles
EDAT- 2021/06/03 06:00
MHDA- 2021/06/03 06:01
CRDT- 2021/06/02 05:41
PHST- 2021/01/25 00:00 [received]
PHST- 2021/03/08 00:00 [revised]
PHST- 2021/03/11 00:00 [accepted]
PHST- 2021/06/02 05:41 [entrez]
PHST- 2021/06/03 06:00 [pubmed]
PHST- 2021/06/03 06:01 [medline]
AID - S0039-9140(21)00229-0 [pii]
AID - 10.1016/j.talanta.2021.122308 [doi]
PST - ppublish
SO  - Talanta. 2021 Sep 1;232:122308. doi: 10.1016/j.talanta.2021.122308. Epub 2021 Mar 
      18.