PMID- 34076786 OWN - NLM STAT- MEDLINE DCOM- 20220105 LR - 20220910 IS - 1436-3305 (Electronic) IS - 1436-3291 (Linking) VI - 24 IP - 6 DP - 2021 Nov TI - Propionibacterium acnes overabundance in gastric cancer promote M2 polarization of macrophages via a TLR4/PI3K/Akt signaling. PG - 1242-1253 LID - 10.1007/s10120-021-01202-8 [doi] AB - OBJECTIVE: Eradication of Helicobacter pylori (H. pylori) could not completely prevent the progression of gastric cancer (GC), suggesting that non-H. pylori bacteria may participate in the carcinogenesis of GC. The dysbiosis of microbiota in the stomach of GC has gradually been investigated, while the detailed mechanism that promotes GC in this process has not been elucidated. We aimed to identify a non-H. pylori bacteria that contribute to GC. DESIGN: GC tissues and adjacent normal tissues were collected to identify bacteria that significantly increased in GC tissues by 16S rRNA gene sequencing and fluorescence in situ hybridization (FISH) analysis. CCK8, wound healing assay, and trans-well assay were performed to analyze the tumor-promoting effect of this bacteria. Next, we detailed the mechanism for tumor-promoting effect of the bacteria by immunofluorescence, RT-qPCR, and western-blotting analysis. RESULTS: Comparing the microbial community from GC tissues and adjacent normal tissues, we found that Propionibacterium acnes (P. acnes) significantly increased in GC tissues, especially in H. pylori-negative tissues. We further found that the abundance of P. acnes correlated with TNM stages of GC patients. Interestingly, condition medium (CM) from P. acnes-primed macrophages promoted migration of GC cells, while P. acnes only could not. We next proved that P. acnes triggers M2 polarization of macrophages via TLR4/PI3K/Akt signaling. CONCLUSIONS: Together, our finding identified that P. acnes could be a possible agent for the progression of GC besides H. pylori. M2 polarization of macrophages could be promoted by P. acnes via TLR4/PI3K/Akt signaling, thus triggers the progression of GC. CI - (c) 2021. The International Gastric Cancer Association and The Japanese Gastric Cancer Association. FAU - Li, Qing AU - Li Q AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Key Laboratory of Digestive System Disease, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Jiefang Rd. 238, Wuhan, 430060, People's Republic of China. FAU - Wu, Wei AU - Wu W AD - Department of Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China. FAU - Gong, Dexin AU - Gong D AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Key Laboratory of Digestive System Disease, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Jiefang Rd. 238, Wuhan, 430060, People's Republic of China. FAU - Shang, Renduo AU - Shang R AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Key Laboratory of Digestive System Disease, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Jiefang Rd. 238, Wuhan, 430060, People's Republic of China. FAU - Wang, Jing AU - Wang J AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Key Laboratory of Digestive System Disease, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Jiefang Rd. 238, Wuhan, 430060, People's Republic of China. FAU - Yu, Honggang AU - Yu H AUID- ORCID: 0000-0002-1882-7060 AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. yuhonggang@whu.edu.cn. AD - Hubei Key Laboratory of Digestive System Disease, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. yuhonggang@whu.edu.cn. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Jiefang Rd. 238, Wuhan, 430060, People's Republic of China. yuhonggang@whu.edu.cn. LA - eng PT - Journal Article DEP - 20210602 PL - Japan TA - Gastric Cancer JT - Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association JID - 100886238 RN - 0 (Toll-Like Receptor 4) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SB - IM EIN - Gastric Cancer. 2022 Nov;25(6):1139-1141. PMID: 36087162 MH - Dysbiosis MH - Humans MH - Macrophages/*metabolism MH - Phosphatidylinositol 3-Kinases/metabolism MH - Propionibacterium acnes/*metabolism MH - Proto-Oncogene Proteins c-akt/metabolism MH - Signal Transduction MH - Stomach Neoplasms/*microbiology MH - Toll-Like Receptor 4/metabolism OTO - NOTNLM OT - Gastric cancer OT - Macrophage OT - Microbiota OT - Propionibacterium acnes EDAT- 2021/06/03 06:00 MHDA- 2022/01/06 06:00 CRDT- 2021/06/02 12:24 PHST- 2021/03/22 00:00 [received] PHST- 2021/05/27 00:00 [accepted] PHST- 2021/06/03 06:00 [pubmed] PHST- 2022/01/06 06:00 [medline] PHST- 2021/06/02 12:24 [entrez] AID - 10.1007/s10120-021-01202-8 [pii] AID - 10.1007/s10120-021-01202-8 [doi] PST - ppublish SO - Gastric Cancer. 2021 Nov;24(6):1242-1253. doi: 10.1007/s10120-021-01202-8. Epub 2021 Jun 2.