PMID- 3407778
OWN - NLM
STAT- MEDLINE
DCOM- 19880914
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 255
IP  - 2 Pt 1
DP  - 1988 Aug
TI  - Intestinal anaphylaxis: in vivo and in vitro studies of the rat proximal colon.
PG  - G201-5
AB  - The response of the rat proximal colon to an immunoglobulin E (IgE)-mediated 
      hypersensitivity reaction was examined. Rats were sensitized to egg albumin (EA) 
      by intraperitoneal injection, and serum titers of specific anti-EA IgE were 
      measured at 14 days. Sensitized animals had titers of greater than or equal to 
      1:64, whereas no anti-EA IgE antibodies were detected in controls. Water and 
      electrolyte absorption in the proximal colon, before and during antigen 
      challenge, was measured by in vivo marker perfusion. Antigen challenge resulted 
      in significant inhibition of water, Na+, Cl-, and K+ absorption in vivo. Proximal 
      colonic tissue from sensitized and control animals was studied in Ussing chambers 
      under short-circuited conditions. Antigen challenge of sensitized tissue resulted 
      in significant increases in short-circuit current due to the induction of active 
      Cl- secretion. No such changes were seen in control tissue. The abnormalities 
      induced by antigen challenge in tissue from sensitized animals was blocked by 
      doxantrazole (10(-3) M), a mast cell stabilizer. The findings indicate that 
      IgE-mediated reactions in rat proximal colon to a food protein cause pertubations 
      in water and electrolyte transport secondary to active Cl- secretion and these 
      abnormalities appear to be due to mast cell degranulation.
FAU - Forbes, D
AU  - Forbes D
AD  - Department of Pediatrics, University of Calgary, Alberta, Canada.
FAU - Patrick, M
AU  - Patrick M
FAU - Perdue, M
AU  - Perdue M
FAU - Buret, A
AU  - Buret A
FAU - Gall, D G
AU  - Gall DG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - *Anaphylaxis
MH  - Animals
MH  - Antibody Formation
MH  - Colon/*immunology/physiology
MH  - Epithelium/physiology
MH  - Female
MH  - Immunoglobulin E/*analysis
MH  - In Vitro Techniques
MH  - Membrane Potentials
MH  - Ovalbumin/immunology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reference Values
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
AID - 10.1152/ajpgi.1988.255.2.G201 [doi]
PST - ppublish
SO  - Am J Physiol. 1988 Aug;255(2 Pt 1):G201-5. doi: 10.1152/ajpgi.1988.255.2.G201.