PMID- 34079542 OWN - NLM STAT- MEDLINE DCOM- 20211006 LR - 20211006 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 12 DP - 2021 TI - Global Gene Expression of T Cells Is Differentially Regulated by Peritoneal Dendritic Cell Subsets in an IL-2 Dependent Manner. PG - 648348 LID - 10.3389/fimmu.2021.648348 [doi] LID - 648348 AB - Dendritic cells (DCs) in peripheral tissues may have a unique role to regulate innate and adaptive immune responses to antigens that enter the tissues. Peritoneal cavity is the body compartment surrounding various tissues and organs and housing diverse immune cells. Here, we investigated the specialized features of classical DC (cDC) subsets following the intraperitoneal injection of a model antigen ovalbumin (OVA). Peritoneal cDC1s were superior to cDC2s in activating OVA-specific CD8 T cells, while both cDCs were similar in stimulating OVA-specific CD4 T cells. Each peritoneal cDC subset differentially regulated the homing properties of CD8 T cells. CD8 T cells stimulated by cDC1s displayed a higher level of lung-homing receptor CCR4, whereas those stimulated by cDC2s prominently expressed various homing receptors including gut-homing molecules CCR9 and alpha4beta7. Also, we found that cDC1s played a dominating role over cDC2s in controlling the overall gene expression of CD8 T cells. Soluble factor(s) emanating from CD8 T cells stimulated by peritoneal cDC1s were responsible for mediating this dominance of cDC1s, and we identified IL-2 as a soluble factor regulating the global gene expression of T cells. Collectively, our study indicates that different peritoneal cDC subsets effectively diversify T cell responses by altering the level of cytokines, such as IL-2, in the milieu. CI - Copyright (c) 2021 Sohn, Na, Shin, Ryu, Park, In, Choi, Park, Hwang, Chu and Park. FAU - Sohn, Moah AU - Sohn M AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - Na, Hye Young AU - Na HY AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Department of Neurology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea. FAU - Shin, Hyun Soo AU - Shin HS AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - Ryu, Seul Hye AU - Ryu SH AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - Park, Sejung AU - Park S AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - In, Hyunju AU - In H AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - Choi, Wanho AU - Choi W AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - Park, Ji Soo AU - Park JS AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - Hwang, Soomin AU - Hwang S AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. FAU - Chu, Min Kyung AU - Chu MK AD - Department of Neurology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea. FAU - Park, Chae Gyu AU - Park CG AD - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea. AD - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. AD - Therapeutic Antibody Research Center, GENUV Inc., Seoul, South Korea. AD - Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, South Korea. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210517 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Antigens) RN - 0 (CC chemokine receptor 9) RN - 0 (Ccr4 protein, mouse) RN - 0 (Interleukin-2) RN - 0 (Receptors, CCR) RN - 0 (Receptors, CCR4) RN - 9006-59-1 (Ovalbumin) SB - IM MH - Animals MH - Antigen Presentation/drug effects MH - Antigens/administration & dosage MH - CD4-Positive T-Lymphocytes/drug effects/*immunology MH - CD8-Positive T-Lymphocytes/drug effects/*immunology MH - Cell Communication/*genetics MH - Cells, Cultured MH - Dendritic Cells/drug effects/*immunology MH - Female MH - Gene Expression/drug effects MH - *Gene Expression Regulation/drug effects MH - Interleukin-2/*metabolism/pharmacology MH - Mice MH - Mice, Inbred C57BL MH - Ovalbumin/administration & dosage MH - Peritoneal Cavity/*cytology MH - Receptors, CCR/metabolism MH - Receptors, CCR4/metabolism PMC - PMC8165281 OTO - NOTNLM OT - T cell - DC interactions OT - antigen presentation OT - dendritic cell OT - interleukin-2 OT - peritoneal cavity COIS- CP is employed by GENUV Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2021/06/04 06:00 MHDA- 2021/10/07 06:00 PMCR- 2021/01/01 CRDT- 2021/06/03 06:40 PHST- 2020/12/31 00:00 [received] PHST- 2021/04/29 00:00 [accepted] PHST- 2021/06/03 06:40 [entrez] PHST- 2021/06/04 06:00 [pubmed] PHST- 2021/10/07 06:00 [medline] PHST- 2021/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2021.648348 [doi] PST - epublish SO - Front Immunol. 2021 May 17;12:648348. doi: 10.3389/fimmu.2021.648348. eCollection 2021.