PMID- 34079566
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240402
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - Commonly and Specifically Activated Defense Responses in Maize Disease Lesion 
      Mimic Mutants Revealed by Integrated Transcriptomics and Metabolomics Analysis.
PG  - 638792
LID - 10.3389/fpls.2021.638792 [doi]
LID - 638792
AB  - Disease lesion mimic (Les/les) mutants display disease-like spontaneous lesions 
      in the absence of pathogen infection, implying the constitutive activation of 
      defense responses. However, the genetic and biochemical bases underlying the 
      activated defense responses in those mutants remain largely unknown. Here, we 
      performed integrated transcriptomics and metabolomics analysis on three typical 
      maize Les mutants Les4, Les10, and Les17 with large, medium, and small lesion 
      size, respectively, thereby dissecting the activated defense responses at the 
      transcriptional and metabolomic level. A total of 1,714, 4,887, and 1,625 
      differentially expressed genes (DEGs) were identified in Les4, Les10, and Les17, 
      respectively. Among them, 570, 3,299, and 447 specific differentially expressed 
      genes (SGs) were identified, implying a specific function of each LES gene. In 
      addition, 480 common differentially expressed genes (CGs) and 42 common 
      differentially accumulated metabolites (CMs) were identified in all Les mutants, 
      suggesting the robust activation of shared signaling pathways. Intriguingly, 
      substantial analysis of the CGs indicated that genes involved in the programmed 
      cell death, defense responses, and phenylpropanoid and terpenoid biosynthesis 
      were most commonly activated. Genes involved in photosynthetic biosynthesis, 
      however, were generally repressed. Consistently, the dominant CMs identified were 
      phenylpropanoids and flavonoids. In particular, lignin, the phenylpropanoid-based 
      polymer, was significantly increased in all three mutants. These data 
      collectively imply that transcriptional activation of defense-related gene 
      expression; increase of phenylpropanoid, lignin, flavonoid, and terpenoid 
      biosynthesis; and inhibition of photosynthesis are generalnatures associated with 
      the lesion formation and constitutively activated defense responses in those 
      mutants. Further studies on the identified SGs and CGs will shed new light on the 
      function of each LES gene as well as the regulatory network of defense responses 
      in maize.
CI  - Copyright (c) 2021 Mu, Li, Dai, Xu, Fan, Jing, Chen and Gou.
FAU - Mu, Xiaohuan
AU  - Mu X
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Li, Jiankun
AU  - Li J
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Dai, Zhuangzhuang
AU  - Dai Z
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Xu, Liping
AU  - Xu L
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Fan, Tianyuan
AU  - Fan T
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Jing, Teng
AU  - Jing T
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Chen, Mengyao
AU  - Chen M
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Gou, Mingyue
AU  - Gou M
AD  - State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation 
      Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, 
      Zhengzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210517
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC8165315
OTO - NOTNLM
OT  - defense responses
OT  - disease lesion mimics
OT  - lignin
OT  - maize
OT  - metabolomics
OT  - phenylpropanoid
OT  - terpenoid
OT  - transcriptomics
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/06/04 06:00
MHDA- 2021/06/04 06:01
PMCR- 2021/01/01
CRDT- 2021/06/03 06:40
PHST- 2020/12/07 00:00 [received]
PHST- 2021/03/29 00:00 [accepted]
PHST- 2021/06/03 06:40 [entrez]
PHST- 2021/06/04 06:00 [pubmed]
PHST- 2021/06/04 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.638792 [doi]
PST - epublish
SO  - Front Plant Sci. 2021 May 17;12:638792. doi: 10.3389/fpls.2021.638792. 
      eCollection 2021.