PMID- 34081253
OWN - NLM
STAT- MEDLINE
DCOM- 20220202
LR  - 20220202
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 44
IP  - 5
DP  - 2021 Oct
TI  - Propofol Regulates the TLR4/NF-kappaB Pathway Through miRNA-155 to Protect Colorectal 
      Cancer Intestinal Barrier.
PG  - 2078-2090
LID - 10.1007/s10753-021-01485-0 [doi]
AB  - Surgery for colorectal cancer (CRC) can cause damage to the intestinal mucosal 
      barrier and lead to bacterial invasion. This study mainly analyzed whether 
      propofol (PPF) could protect the intestinal mucosal barrier damage caused by CRC 
      surgery, and explored its molecular mechanism. A mouse CRC model was constructed 
      using azomethane and dextran sulfate sodium. During anesthesia, continuous 
      intravenous injection of PPF was used for intervention. The influences of PPF on 
      intestinal mucosal permeability and bacterial invasion were detected. The levels 
      of microRNA (miR)-155, Toll-like receptor 4 (TLR4)/NF-kappaB in the intestinal 
      mucosa, and the location of miR-155 were detected by fluorescence in situ 
      hybridization (FISH). Mouse macrophages were used to analyze the regulation of 
      miR-155 on the secretion of inflammatory cytokines through the TLR4/NF-kappaB 
      pathway. PPF treatment promoted the expression of tight junction protein in the 
      intestinal mucosa, protected the intestinal barrier, inhibited the translocation 
      of intestinal bacteria, and increased the level of the beneficial bacterium 
      Lactobacillus on the mucosal surface. In addition, PPF treatment could inhibit 
      the expression of miR-155, TLR4/NF-KB, and reverse inflammatory response. miR-155 
      was expressed in macrophages of intestinal mucosa tissue. Overexpression of 
      miR-155 promoted the nuclear translocation of NF-kappaB and the expression of 
      inflammatory cytokines in macrophages. The use of VIPER to inhibit TLR4 reversed 
      the pro-inflammatory effects of miR-155. PPF might inhibit the activation of the 
      NF-kappaB pathway by downregulating miR-155 expression, thereby reducing the 
      secretion of inflammatory cytokines. This might be the mechanism by which PPF 
      protected the intestinal barrier of CRC surgical model mice.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Gao, Yuhua
AU  - Gao Y
AD  - Department of Anesthesiology, General Hospital of Ningxia Medical University, No. 
      804 Shengli South Street, Xingqing District, Yinchuan, 750004, Ningxia, China.
AD  - School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Han, Tao
AU  - Han T
AD  - Department of Ultrasound, General Hospital of Ningxia Medical University, 
      Yinchuan, Ningxia, China.
FAU - Han, Cailing
AU  - Han C
AD  - Department of Anesthesiology, General Hospital of Ningxia Medical University, No. 
      804 Shengli South Street, Xingqing District, Yinchuan, 750004, Ningxia, China.
FAU - Sun, Hua
AU  - Sun H
AD  - Department of Anesthesiology, General Hospital of Ningxia Medical University, No. 
      804 Shengli South Street, Xingqing District, Yinchuan, 750004, Ningxia, China.
FAU - Yang, Xiaoxia
AU  - Yang X
AD  - Department of Anesthesiology, General Hospital of Ningxia Medical University, No. 
      804 Shengli South Street, Xingqing District, Yinchuan, 750004, Ningxia, China.
FAU - Zhang, Dongmei
AU  - Zhang D
AD  - Department of Anesthesiology, General Hospital of Ningxia Medical University, No. 
      804 Shengli South Street, Xingqing District, Yinchuan, 750004, Ningxia, China.
FAU - Ni, Xinli
AU  - Ni X
AUID- ORCID: 0000-0002-1330-3620
AD  - Department of Anesthesiology, General Hospital of Ningxia Medical University, No. 
      804 Shengli South Street, Xingqing District, Yinchuan, 750004, Ningxia, China. 
      xinlini6@nyfy.com.cn.
LA  - eng
GR  - 2019A0206/Natural Science Foundation of Ningxia Province/
GR  - XM2018170/Ningxia Medical University Foundation Project/
PT  - Journal Article
DEP - 20210603
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Hypnotics and Sedatives)
RN  - 0 (Inflammation Mediators)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - YI7VU623SF (Propofol)
SB  - IM
MH  - Animals
MH  - Colorectal Neoplasms/drug therapy/*metabolism/pathology
MH  - Hypnotics and Sedatives/pharmacology/therapeutic use
MH  - Inflammation Mediators/antagonists & inhibitors/metabolism
MH  - Intestinal Mucosa/drug effects/*metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*antagonists & inhibitors/metabolism
MH  - NF-kappa B/*antagonists & inhibitors/metabolism
MH  - Propofol/pharmacology/*therapeutic use
MH  - RAW 264.7 Cells
MH  - Signal Transduction/drug effects/physiology
MH  - Toll-Like Receptor 4/*antagonists & inhibitors/metabolism
OTO - NOTNLM
OT  - Toll-like receptor 4 (TLR4)/NF-kappaB
OT  - colorectal cancer
OT  - intestinal barrier
OT  - microRNA-155
OT  - propofol
EDAT- 2021/06/04 06:00
MHDA- 2022/02/03 06:00
CRDT- 2021/06/03 12:31
PHST- 2021/04/08 00:00 [received]
PHST- 2021/05/23 00:00 [accepted]
PHST- 2021/05/11 00:00 [revised]
PHST- 2021/06/04 06:00 [pubmed]
PHST- 2022/02/03 06:00 [medline]
PHST- 2021/06/03 12:31 [entrez]
AID - 10.1007/s10753-021-01485-0 [pii]
AID - 10.1007/s10753-021-01485-0 [doi]
PST - ppublish
SO  - Inflammation. 2021 Oct;44(5):2078-2090. doi: 10.1007/s10753-021-01485-0. Epub 
      2021 Jun 3.