PMID- 34083583
OWN - NLM
STAT- MEDLINE
DCOM- 20211129
LR  - 20211129
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jun 3
TI  - Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by 
      the subcutaneous route.
PG  - 11769
LID - 10.1038/s41598-021-90979-w [doi]
LID - 11769
AB  - To study the pathogenicity of new duck reovirus (NDRV) to chickens, eighty 
      3-day-old SPF chickens were equally divided into two groups. The experimental 
      group was inoculated with a NDRV challenge strain of 100 muL (10(-5.00) 
      ELD(50)/0.1 mL) by the subcutaneous (s.c.) route, and the control group was 
      inoculated with 100 muL of sterile phosphate-buffered saline (PBS) by the same 
      route. In the experimental group, chickens exhibited introflexion of claws, 
      performing of splits, stunting syndrome, weight loss and death. Gross lesions 
      such as enlargement and yellowish-white focal necroses were observed in the liver 
      and spleen. Microscopic changes were typical including varying degrees of 
      hepatocyte steatosis and necrosis, splenic lymphocyte necrosis, interstitial 
      pneumonia. Viral loads were detected in lung, liver, heart, spleen, duodenum, 
      burse and kidney. The liver and spleen viral loads remained a much higher level 
      and maintained for a longer time, suggesting that these tissues might be the 
      target organs. In summary, NDRV can cause systemic infections and death in 
      chickens, which indicated that chickens may be infected by NDRV in 
      poultry production.
FAU - Yu, Kexiang
AU  - Yu K
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Ti, Jinfeng
AU  - Ti J
AD  - Shandong Vocational Animal Science and Veterinary College, Jinan City, Shan Dong 
      Province, China.
FAU - Lu, Xiao
AU  - Lu X
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Pan, Li
AU  - Pan L
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Liu, Liping
AU  - Liu L
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Gao, Yuehua
AU  - Gao Y
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Guo, Xiaozhen
AU  - Guo X
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Hu, Feng
AU  - Hu F
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Liu, Cunxia
AU  - Liu C
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Ma, Xiuli
AU  - Ma X
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Li, Yufeng
AU  - Li Y
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China.
FAU - Huang, Bing
AU  - Huang B
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China. hbind@163.com.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China. hbind@163.com.
FAU - Song, Minxun
AU  - Song M
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Sciences, No. 1 
      Jiaoxiao Road, Jinan City, 250023, Shan Dong Province, China. mxsong@aliyun.com.
AD  - Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, 
      Jinan City, Shan Dong Province, China. mxsong@aliyun.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210603
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood/immunology
MH  - Antibodies, Viral/blood/immunology
MH  - Biopsy
MH  - *Chickens
MH  - Immunohistochemistry
MH  - Mortality
MH  - Poultry Diseases/diagnosis/mortality/*transmission/*virology
MH  - Reoviridae/classification/immunology/*pathogenicity
MH  - Reoviridae Infections/*veterinary
MH  - Viral Load
PMC - PMC8175558
COIS- The authors declare no competing interests.
EDAT- 2021/06/05 06:00
MHDA- 2021/11/30 06:00
PMCR- 2021/06/03
CRDT- 2021/06/04 06:24
PHST- 2020/11/16 00:00 [received]
PHST- 2021/04/15 00:00 [accepted]
PHST- 2021/06/04 06:24 [entrez]
PHST- 2021/06/05 06:00 [pubmed]
PHST- 2021/11/30 06:00 [medline]
PHST- 2021/06/03 00:00 [pmc-release]
AID - 10.1038/s41598-021-90979-w [pii]
AID - 90979 [pii]
AID - 10.1038/s41598-021-90979-w [doi]
PST - epublish
SO  - Sci Rep. 2021 Jun 3;11(1):11769. doi: 10.1038/s41598-021-90979-w.