PMID- 34085156
OWN - NLM
STAT- MEDLINE
DCOM- 20231006
LR  - 20231006
IS  - 1438-2199 (Electronic)
IS  - 0939-4451 (Linking)
VI  - 54
IP  - 3
DP  - 2022 Mar
TI  - Protective effects of glycine against lipopolysaccharide-induced intestinal 
      apoptosis and inflammation.
PG  - 353-364
LID - 10.1007/s00726-021-03011-w [doi]
AB  - Intestinal dysfunction is commonly observed in humans and animals. Glycine (Gly) 
      is a functional amino acid with anti-inflammatory and anti-apoptotic properties. 
      The objective of this study was to test the protective effects of Gly against 
      lipopolysaccharide (LPS)-induced intestinal injury. 28 C57BL/6 mice with a body 
      weight (BW) of 18 +/- 2 g were randomly assigned into four groups: CON (control), 
      GLY (orally administered Gly, 5 g/kg BW/day for 6 days), LPS (5 mg/kg BW on day 
      7, i. p.), and GLY + LPS (Gly pretreatment and LPS administration). Histological 
      alterations, inflammatory responses, epithelial cell apoptosis, and changes of 
      the intestinal microbiota were analyzed. Results showed that, compared with the 
      CON group, mice in the LPS treatment group showed decreased villus height, 
      increased crypt depth, and decreased ratio of villus height to crypt depth, which 
      were significantly attenuated by Gly. Neither LPS nor Gly treatment altered 
      morphology of the distal colon tissues. LPS increased the apoptosis of jejunum 
      and colon epithelial cells and protein abundance of cleaved caspase3 in the 
      jejunum, which were markedly abrogated by Gly. LPS also elevated the mRNA levels 
      of Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MYD88), 
      pro-inflammatory cytokines, and chemokines in the jejunum and colon. These 
      alterations were significantly suppressed by Gly. In addition, Gly 
      supplementation attenuated infiltration of CD4(+), CD8(+) T-lymphocytes, CD11b(+) 
      and F4/80(+) macrophages in the colon. Furthermore, Gly increased the relative 
      abundance of Mucispirillum, Lachnospiraceae-NK4A136-group, Anaerotruncus, 
      Faecalibaculum, Ruminococcaceae-UCG-014, and decreased the abundance of 
      Bacteroides at genus level. Supplementation with Gly might be a nutritional 
      strategy to ameliorate LPS-induced intestinal injury in mice.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Zhang, Yunchang
AU  - Zhang Y
AD  - State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed 
      Science, China Agricultural University, Beijing, 100193, China.
FAU - Mu, Tianqi
AU  - Mu T
AD  - State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed 
      Science, China Agricultural University, Beijing, 100193, China.
FAU - Jia, Hai
AU  - Jia H
AD  - State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed 
      Science, China Agricultural University, Beijing, 100193, China.
FAU - Yang, Ying
AU  - Yang Y
AD  - State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed 
      Science, China Agricultural University, Beijing, 100193, China.
FAU - Wu, Zhenlong
AU  - Wu Z
AUID- ORCID: 0000-0001-9700-8874
AD  - State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed 
      Science, China Agricultural University, Beijing, 100193, China. 
      bio2046@hotmail.com.
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, China 
      Agricultural University, Beijing, 100193, China. bio2046@hotmail.com.
LA  - eng
GR  - 31572412/National Natural Science Foundation of China/
GR  - 31572410/National Natural Science Foundation of China/
GR  - 31625025/National Natural Science Foundation of China/
GR  - 31272450/National Natural Science Foundation of China/
GR  - 31272451/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210604
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - TE7660XO1C (Glycine)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Apoptosis
MH  - *Glycine/metabolism
MH  - Inflammation/chemically induced/drug therapy/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - *Lipopolysaccharides/metabolism/toxicity
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - Apoptosis
OT  - Glycine
OT  - Inflammatory responses
OT  - Intestinal injury
OT  - Lipopolysaccharide
EDAT- 2021/06/05 06:00
MHDA- 2022/04/05 06:00
CRDT- 2021/06/04 07:17
PHST- 2021/01/31 00:00 [received]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/06/05 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2021/06/04 07:17 [entrez]
AID - 10.1007/s00726-021-03011-w [pii]
AID - 10.1007/s00726-021-03011-w [doi]
PST - ppublish
SO  - Amino Acids. 2022 Mar;54(3):353-364. doi: 10.1007/s00726-021-03011-w. Epub 2021 
      Jun 4.