PMID- 34085315
OWN - NLM
STAT- MEDLINE
DCOM- 20220210
LR  - 20220210
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 99
IP  - 9
DP  - 2021 Sep
TI  - Efficient extra-mitochondrial aerobic ATP synthesis in neuronal membrane systems.
PG  - 2250-2260
LID - 10.1002/jnr.24865 [doi]
AB  - The nervous system displays high energy consumption, apparently not fulfilled by 
      mitochondria, which are underrepresented therein. The oxidative phosphorylation 
      (OxPhos) activity, a mitochondrial process that aerobically provides ATP, has 
      also been reported also in the myelin sheath and the rod outer segment (OS) 
      disks. Thus, commonalities and differences between the extra-mitochondrial and 
      mitochondrial aerobic metabolism were evaluated in bovine isolated myelin (IM), 
      rod OS, and mitochondria-enriched fractions (MIT). The subcellular fraction 
      quality and the absence of contamination fractions have been estimated by western 
      blot analysis. Oxygen consumption and ATP synthesis were stimulated by 
      conventional (pyruvate + malate or succinate) and unconventional (NADH) 
      substrates, observing that oxygen consumption and ATP synthesis by IM and rod OS 
      are more efficient than by MIT, in the presence of both kinds of respiratory 
      substrates. Mitochondria did not utilize NADH as a respiring substrate. When ATP 
      synthesis by either sample was assayed in the presence of 10-100 microM ATP in the 
      assay medium, only in IM and OS it was not inhibited, suggesting that the ATP 
      exportation by the mitochondria is limited by extravesicular ATP concentration. 
      Interestingly, IM and OS but not mitochondria appear able to synthesize ATP at a 
      later time with respect to exposure to respiratory substrates, supporting the 
      hypothesis that the proton gradient produced by the electron transport chain is 
      buffered by membrane phospholipids. The putative transfer mode of the OxPhos 
      molecular machinery from mitochondria to the extra-mitochondrial structures is 
      also discussed, opening new perspectives in the field of neurophysiology.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Ravera, Silvia
AU  - Ravera S
AUID- ORCID: 0000-0002-0803-1042
AD  - Department of Experimental Medicine, University of Genoa, Genoa, Italy.
FAU - Bartolucci, Martina
AU  - Bartolucci M
AUID- ORCID: 0000-0001-5289-4219
AD  - Laboratory of Mass Spectrometry - Core Facilities, Istituto Giannina Gaslini, 
      Genoa, Italy.
AD  - Department of Pharmacy, Biochemistry Lab., University of Genoa, Genoa, Italy.
FAU - Calzia, Daniela
AU  - Calzia D
AD  - Department of Pharmacy, Biochemistry Lab., University of Genoa, Genoa, Italy.
FAU - Morelli, Alessandro M
AU  - Morelli AM
AD  - Department of Pharmacy, Biochemistry Lab., University of Genoa, Genoa, Italy.
FAU - Panfoli, Isabella
AU  - Panfoli I
AUID- ORCID: 0000-0002-6261-1128
AD  - Department of Pharmacy, Biochemistry Lab., University of Genoa, Genoa, Italy.
LA  - eng
PT  - Journal Article
DEP - 20210603
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/administration & dosage/*biosynthesis
MH  - Animals
MH  - Cattle
MH  - Cell Membrane/drug effects/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Male
MH  - Mitochondria/drug effects/*metabolism
MH  - Neurons/drug effects/*metabolism
MH  - Oxygen Consumption/drug effects/physiology
MH  - Prosencephalon/drug effects/*metabolism
MH  - Retina/drug effects/*metabolism
OTO - NOTNLM
OT  - RRID:AB_10696805
OT  - RRID:AB_11183050
OT  - RRID:AB_1845182
OT  - RRID:AB_2818988
OT  - RRID:AB_2851910
OT  - RRID:SCR_002798
OT  - RRID:SCR_014210
OT  - aerobic metabolism
OT  - bioenergetics
OT  - myelin sheath
OT  - oxidative phosphorylation
OT  - phototransduction
OT  - rod outer segments disks
EDAT- 2021/06/05 06:00
MHDA- 2022/02/11 06:00
CRDT- 2021/06/04 07:24
PHST- 2021/04/29 00:00 [revised]
PHST- 2020/05/13 00:00 [received]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/06/05 06:00 [pubmed]
PHST- 2022/02/11 06:00 [medline]
PHST- 2021/06/04 07:24 [entrez]
AID - 10.1002/jnr.24865 [doi]
PST - ppublish
SO  - J Neurosci Res. 2021 Sep;99(9):2250-2260. doi: 10.1002/jnr.24865. Epub 2021 Jun 
      3.