PMID- 34087504
OWN - NLM
STAT- MEDLINE
DCOM- 20210721
LR  - 20210721
IS  - 1873-3700 (Electronic)
IS  - 0031-9422 (Linking)
VI  - 189
DP  - 2021 Sep
TI  - Genome-wide identification and characterization of genes involved in melatonin 
      biosynthesis in Morus notabilis (wild mulberry).
PG  - 112819
LID - S0031-9422(21)00168-0 [pii]
LID - 10.1016/j.phytochem.2021.112819 [doi]
AB  - Melatonin is recognized as an important regulator for human health and widely 
      distributed in many plant species, including mulberry (Morus L.). Previous 
      studies suggested mulberry contains high melatonin content, but the molecular 
      mechanisms underlying melatonin biosynthesis in mulberry remain unclear. Here, 37 
      genes involved in melatonin biosynthesis were identified in mulberry genome, 
      including a tryptophan decarboxylase gene (MnTDC), seven tryptophan 5-hydroxylase 
      genes (MnT5Hs), six serotonin N-acetyltransferase genes (MnSNATs), 
      20 N-acetylserotonin methyltransferase genes (MnASMTs) and three caffeic acid 
      3-O-methyltransferase genes (MnCOMTs). Expression analysis showed that MnTDC, 
      MnT5H2, MnSNAT5, MnASMT12 and MnCOMT1 from these genes had highest expression 
      levels within their corresponding families. In vitro enzymatic assays indicated 
      that MnTDC, MnT5H2, MnSNAT5, MnASMT12 and MnCOMT1 play important roles in 
      melatonin biosynthesis. Multiple different pathways for melatonin biosynthesis in 
      mulberry were discovered. In addition, mulberry ASMT showed distinct roles with 
      those of ASTMs in Arabidopsis and rice. The class I ASMT, MnASMT12, and the class 
      III ASMT, MnASMT20, catalyzed the conversion of N-acetylserotonin to melatonin 
      and serotonin to 5-methoxytryptamine. Furthermore, the class II ASMT, MnASMT16, 
      only catalyzed the conversion of N-acetylserotonin to melatonin. This study 
      improved our knowledge on melatonin biosynthesis in mulberry and expands the 
      repertoire of melatonin biosynthesis pathways in plants.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Zheng, Sha
AU  - Zheng S
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, 
      Chongqing, 400716, China.
FAU - Zhu, Yingxue
AU  - Zhu Y
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, 
      Chongqing, 400716, China.
FAU - Liu, Changying
AU  - Liu C
AD  - Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural 
      Affairs, Chengdu University, Chengdu, 610106, China.
FAU - Fan, Wei
AU  - Fan W
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, 
      Chongqing, 400716, China.
FAU - Xiang, Zhonghuai
AU  - Xiang Z
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, 
      Chongqing, 400716, China.
FAU - Zhao, Aichun
AU  - Zhao A
AD  - State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural 
      Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, 
      Chongqing, 400716, China. Electronic address: zhaoaichun@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210601
PL  - England
TA  - Phytochemistry
JT  - Phytochemistry
JID - 0151434
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - *Arabidopsis
MH  - *Melatonin
MH  - *Morus/genetics
MH  - *Oryza
OTO - NOTNLM
OT  - ASMT
OT  - Enzymatic assay
OT  - Expression analysis
OT  - Melatonin biosynthetic pathway
OT  - Moraceae
OT  - Morus notabilis
OT  - Mulberry
EDAT- 2021/06/05 06:00
MHDA- 2021/07/22 06:00
CRDT- 2021/06/04 20:17
PHST- 2021/02/25 00:00 [received]
PHST- 2021/04/19 00:00 [revised]
PHST- 2021/05/16 00:00 [accepted]
PHST- 2021/06/05 06:00 [pubmed]
PHST- 2021/07/22 06:00 [medline]
PHST- 2021/06/04 20:17 [entrez]
AID - S0031-9422(21)00168-0 [pii]
AID - 10.1016/j.phytochem.2021.112819 [doi]
PST - ppublish
SO  - Phytochemistry. 2021 Sep;189:112819. doi: 10.1016/j.phytochem.2021.112819. Epub 
      2021 Jun 1.