PMID- 34087673 OWN - NLM STAT- MEDLINE DCOM- 20211104 LR - 20211104 IS - 1873-2542 (Electronic) IS - 0378-1135 (Linking) VI - 259 DP - 2021 Aug TI - Abundance of Lactobacillus in porcine gut microbiota is closely related to immune response following PRRSV immunization. PG - 109134 LID - S0378-1135(21)00157-7 [pii] LID - 10.1016/j.vetmic.2021.109134 [doi] AB - Increasing evidence shows that gut microbiota plays a critical role in host immune system development and immune regulation, thus the composition of gut microbiota may affect how individuals respond to immunizations. Currently, little evidence is available on the correlation between porcine gut microbiota and vaccine immune response. Here, we investigated the influence of gut microbiota on immune response in pigs to porcine reproductive and respiratory syndrome virus (PRRSV) vaccine. Based on the antibody levels for PRRSV, the immunized pigs were divided into three groups (high, low, and others), and followed by virulent PRRSV challenge. The comprehensive analysis of microbial composition revealed that gut microbiota was similar in the richness and diversity among different groups before immunization. After immunization, the richness and diversity of gut microbial community in the high group were still similar to the low group, although there was a decrease in community diversity overtime. Interestingly, the antibody titer was positively correlated with the abundance of Lactobacillus in gut microbiota in immunized pigs. Further analysis indicated that gut microbial composition might be correlated to the clinical parameters such as body weight and rectal temperature after virus challenge. Taken together, our findings suggest that certain specific members of gut microbiota, such as Lactobacillus may serve as a mechanism for regulating the immune response following immunization in pigs. CI - Copyright (c) 2021 Elsevier B.V. All rights reserved. FAU - Zhang, He AU - Zhang H AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China; College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China. Electronic address: zhanghe3789@163.com. FAU - Ma, Wenjie AU - Ma W AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address: qd1992mwj@163.com. FAU - Sun, Zhi AU - Sun Z AD - Asian Veterinary Research and Development Center, Boehringer Ingelheim Vetmedica (China) Co., Ltd., Pudong District, Shanghai, 201203, China. Electronic address: zhi_1.sun@boehringer-ingelheim.com. FAU - Zhu, Changkang AU - Zhu C AD - Asian Veterinary Research and Development Center, Boehringer Ingelheim Vetmedica (China) Co., Ltd., Pudong District, Shanghai, 201203, China. Electronic address: Changkang.zhu@boehringer-ingelheim.com. FAU - Werid, Gebremeskel Mamu AU - Werid GM AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address: ashenafymamo@gmail.com. FAU - Ibrahim, Yassein M AU - Ibrahim YM AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address: yassin8322@gmail.com. FAU - Zhang, Wenli AU - Zhang W AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address: zwl5561@163.com. FAU - Pan, Yu AU - Pan Y AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address: 1628812160@qq.com. FAU - Shi, Dongfang AU - Shi D AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China. Electronic address: shidf@neau.edu.cn. FAU - Chen, Hongyan AU - Chen H AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address: chenhongyan@caas.cn. FAU - Wang, Yue AU - Wang Y AD - State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address: wangyue@caas.cn. LA - eng PT - Journal Article DEP - 20210526 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Antibodies, Viral) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Antibodies, Viral/blood MH - Gastrointestinal Microbiome/*immunology MH - Immunity MH - Immunization/*veterinary MH - Lactobacillus/*immunology/*physiology MH - Porcine Reproductive and Respiratory Syndrome MH - Porcine respiratory and reproductive syndrome virus/*immunology MH - Swine MH - Vaccine Potency MH - Viral Vaccines/administration & dosage/*immunology OTO - NOTNLM OT - Gut microbiota OT - Immune response OT - Lactobacillus OT - PRRSV EDAT- 2021/06/05 06:00 MHDA- 2021/11/05 06:00 CRDT- 2021/06/04 20:26 PHST- 2021/03/25 00:00 [received] PHST- 2021/05/23 00:00 [accepted] PHST- 2021/06/05 06:00 [pubmed] PHST- 2021/11/05 06:00 [medline] PHST- 2021/06/04 20:26 [entrez] AID - S0378-1135(21)00157-7 [pii] AID - 10.1016/j.vetmic.2021.109134 [doi] PST - ppublish SO - Vet Microbiol. 2021 Aug;259:109134. doi: 10.1016/j.vetmic.2021.109134. Epub 2021 May 26.