PMID- 34087674
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 259
DP  - 2021 Aug
TI  - Vimentin rearrangement by phosphorylation is beneficial for porcine reproductive 
      and respiratory syndrome virus replication in vitro.
PG  - 109133
LID - S0378-1135(21)00156-5 [pii]
LID - 10.1016/j.vetmic.2021.109133 [doi]
AB  - Vimentin, a member of intermediate filaments, has been documented to be involved 
      in viral infections. Despite several studies focusing on its involvement in 
      porcine reproductive and respiratory syndrome virus (PRRSV) infection, the 
      detailed mechanisms by which vimentin takes effect remain to be fully elucidated. 
      In the present study, we identified a previously unrecognized role of vimentin 
      rearrangement in PRRSV replication. We monitored that PRRSV infection induced 
      vimentin reorganization during post-entry stage, which was beneficial for viral 
      replication. In detail, the serine residue of vimentin was phosphorylated at 
      position 38 (Ser38) by calcium calmodulin-dependent protein kinase II gamma 
      (CaMKIIgamma), and vimentin filaments reorganized into cage-like structures 
      enwrapping PRRSV replication complex (RC) at the perinuclear location. Taken 
      together, these results expand the knowledge of PRRSV replication, and provide 
      novel targets for prevention and control of PRRSV.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Zheng, Xin-Xian
AU  - Zheng XX
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China.
FAU - Li, Rui
AU  - Li R
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China. Electronic address: lirui860620@sina.com.
FAU - Qiao, Songlin
AU  - Qiao S
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China.
FAU - Chen, Xin-Xin
AU  - Chen XX
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China.
FAU - Zhang, Longxiang
AU  - Zhang L
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China.
FAU - Lu, Qingxia
AU  - Lu Q
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China.
FAU - Xing, Guangxu
AU  - Xing G
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      China.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, China; College of Veterinary Medicine, Henan 
      Agricultural University, Zhengzhou, 450002, China. Electronic address: 
      zhanggaip@126.com.
LA  - eng
PT  - Journal Article
DEP - 20210526
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Vimentin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Phosphorylation
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - Vimentin/chemistry/*metabolism
MH  - *Virus Replication
OTO - NOTNLM
OT  - CaMKIIgamma
OT  - PRRSV
OT  - Rearrangement
OT  - Replication
OT  - Vimentin
EDAT- 2021/06/05 06:00
MHDA- 2021/11/05 06:00
CRDT- 2021/06/04 20:26
PHST- 2021/02/03 00:00 [received]
PHST- 2021/05/21 00:00 [accepted]
PHST- 2021/06/05 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
PHST- 2021/06/04 20:26 [entrez]
AID - S0378-1135(21)00156-5 [pii]
AID - 10.1016/j.vetmic.2021.109133 [doi]
PST - ppublish
SO  - Vet Microbiol. 2021 Aug;259:109133. doi: 10.1016/j.vetmic.2021.109133. Epub 2021 
      May 26.