PMID- 34088592
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 1424-3911 (Electronic)
IS  - 1424-3903 (Linking)
DP  - 2021 May 19
TI  - Decreased infiltration of adipose tissue macrophages and amplified inflammation 
      of adipose tissue in obese mice with severe acute pancreatitis.
LID - S1424-3903(21)00156-3 [pii]
LID - 10.1016/j.pan.2021.05.005 [doi]
AB  - OBJECTIVE: Macrophages are involved in obesity-associated inflammation and severe 
      acute pancreatitis (SAP) development. However, the role of adipose tissue 
      macrophages (ATMs) in obesity-related SAP has not been fully elucidated. We 
      investigated the relationship between ATMs and inflammatory responses in SAP 
      model mice fed a high-fat diet (HFD). METHODS: SAP was induced in animal models 
      via intraperitoneal injections of caerulein and lipopolysaccharide (LPS). SAP 
      severity was evaluated, both morphologically and biochemically, and macrophage 
      infiltration in the pancreas and epididymal adipose tissue was measured. We also 
      analyzed apoptosis levels, polarization of the ATMs, and expression of 
      inflammatory mediators in epididymal adipose tissue. RESULTS: Obesity increased 
      disease severity in SAP animals. Increased macrophage infiltration in the 
      pancreas induced by SAP was found in both normal diet (ND)- and HFD-fed mice. 
      Total ATM infiltration in epididymal adipose tissue was elevated by HFD, while a 
      significant decrease in infiltration was observed in both the ND + SAP and 
      HFD + SAP groups. The apoptosis levels of ATMs were reduced in the HFD group, but 
      were markedly enhanced in both the ND + SAP and HFD + SAP groups compared to 
      their respective control groups. Higher levels of interleukin-6 (IL-6), tumor 
      necrosis factor-alpha (TNF-alpha), and monocyte chemoattractant protein-1 (MCP-1) were 
      observed in the HFD + SAP than in the ND + SAP group. Increased proportion of M1 
      type ATMs was induced by both HFD and SAP. CONCLUSIONS: Total ATM infiltration 
      was decreased in epididymal adipose tissue of SAP animals. ATM polarization to 
      the M1 type resulted in an amplified inflammatory response in obese mice with 
      SAP.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Yu, Qiao
AU  - Yu Q
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Xu, Tao
AU  - Xu T
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Ding, Feifei
AU  - Ding F
AD  - Department of Gastroenterology, Lanzhou University Second Hospital, Lanzhou 
      University, Lanzhou, 730030, China.
FAU - Ding, Zhen
AU  - Ding Z
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China. Electronic address: 
      docd720@126.com.
FAU - Lin, Rong
AU  - Lin R
AD  - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China. Electronic address: 
      selinalin35@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210519
PL  - Switzerland
TA  - Pancreatology
JT  - Pancreatology : official journal of the International Association of 
      Pancreatology (IAP) ... [et al.]
JID - 100966936
SB  - IM
OTO - NOTNLM
OT  - Adipose tissue macrophages
OT  - Apoptosis
OT  - Inflammation
OT  - Obesity
OT  - Severe acute pancreatitis
COIS- Declaration of competing interest All authors state that they have no conflict of 
      interest.
EDAT- 2021/06/06 06:00
MHDA- 2021/06/06 06:00
CRDT- 2021/06/05 05:32
PHST- 2020/08/17 00:00 [received]
PHST- 2021/04/13 00:00 [revised]
PHST- 2021/05/07 00:00 [accepted]
PHST- 2021/06/05 05:32 [entrez]
PHST- 2021/06/06 06:00 [pubmed]
PHST- 2021/06/06 06:00 [medline]
AID - S1424-3903(21)00156-3 [pii]
AID - 10.1016/j.pan.2021.05.005 [doi]
PST - aheadofprint
SO  - Pancreatology. 2021 May 19:S1424-3903(21)00156-3. doi: 10.1016/j.pan.2021.05.005.