PMID- 34090862
OWN - NLM
STAT- MEDLINE
DCOM- 20211011
LR  - 20211011
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 405
IP  - 2
DP  - 2021 Aug 15
TI  - Ferredoxin reductase regulates proliferation, differentiation, cell cycle and 
      lipogenesis but not apoptosis in SZ95 sebocytes.
PG  - 112680
LID - S0014-4827(21)00212-3 [pii]
LID - 10.1016/j.yexcr.2021.112680 [doi]
AB  - Ferredoxin reductase (FDXR), a mitochondrial membrane-associated flavoprotein, is 
      essential for electron transfer and modulates p53-dependent apoptosis in cancer 
      cells.FDXR may be implicated in epidermal and sebocytic differentiation, but its 
      explicit function in sebocytes remains to be elucidated. In the present study, 
      immunohistochemistry revealed that FDXR expression was increased in sebaceous 
      cells of acne lesions. FDXR, PPARgamma, LXRalpha/beta, SREBP1 and Sox9 expression was 
      incremental during sebocyte differentiation. FDXR overexpression induced by 
      Ad-GFP-FDXR infection enhanced differentiation, reactive oxygen species (ROS), 
      lipogenesis and PPARgamma expression, and consequnently inhibited proliferation in 
      SZ95 sebocytes. Flow cytometry showed that FDXR overexpression induced 
      significant blockade of G2/M phase but had no effect on sub-G1 (apoptotic) 
      sebocytes. Insulin-like growth factor-1 (IGF-1)-induced FDXR and PPARgamma expression 
      and lipogenesis were abolished by pretreatment with PI3K inhibitor LY294002. 
      These results suggest that FDXR overexpression might promote differentiation and 
      lipogenesis via ROS production and suppress proliferation via G2/S blockade in 
      SZ95 sebocytes. IGF-1 could facilitate differentiation and lipogenesis through 
      PI3K/Akt/FDXR pathway. FDXR could serve as a potential marker of advanced 
      sebaceous differentiation, and its overexpression may be involved in the 
      development of acne lesions.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Li, Meng-Tong
AU  - Li MT
AD  - Department of Dermatology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, China.
FAU - Pi, Xiao-Xue
AU  - Pi XX
AD  - Department of Dermatology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, China.
FAU - Cai, Xiao-Lin
AU  - Cai XL
AD  - Department of Dermatology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, China.
FAU - Liu, Si
AU  - Liu S
AD  - Department of Dermatology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, China.
FAU - Huang, Zheng
AU  - Huang Z
AD  - Department of Dermatology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, China.
FAU - Zouboulis, Christos C
AU  - Zouboulis CC
AD  - Departments of Dermatology, Venereology, Allergology and Immunology, Dessau 
      Medical Center, Brandenburg Medical School Theodor Fontane, Dessau, Germany.
FAU - Fan, Yi-Ming
AU  - Fan YM
AD  - Department of Dermatology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, China. Electronic address: ymfan1963@163.com.
FAU - Shi, Ge
AU  - Shi G
AD  - Department of Dermatology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, China; Medical Cosmetic and Plastic Surgery Center, The 
      Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China. 
      Electronic address: shig9@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210604
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Ferredoxins)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Cycle/*drug effects
MH  - Cell Differentiation/*drug effects
MH  - Cell Respiration/drug effects
MH  - Epithelial Cells/drug effects
MH  - Ferredoxins/*pharmacology
MH  - Humans
MH  - Lipogenesis/*drug effects
OTO - NOTNLM
OT  - Differentiation
OT  - Ferredoxin reductase
OT  - Insulin-like growth factor 1
OT  - Lipogenesis
OT  - Reactive oxygen species
OT  - Sebocytes
EDAT- 2021/06/07 06:00
MHDA- 2021/10/12 06:00
CRDT- 2021/06/06 20:45
PHST- 2020/10/24 00:00 [received]
PHST- 2021/05/07 00:00 [revised]
PHST- 2021/05/28 00:00 [accepted]
PHST- 2021/06/07 06:00 [pubmed]
PHST- 2021/10/12 06:00 [medline]
PHST- 2021/06/06 20:45 [entrez]
AID - S0014-4827(21)00212-3 [pii]
AID - 10.1016/j.yexcr.2021.112680 [doi]
PST - ppublish
SO  - Exp Cell Res. 2021 Aug 15;405(2):112680. doi: 10.1016/j.yexcr.2021.112680. Epub 
      2021 Jun 4.