PMID- 34091227
OWN - NLM
STAT- MEDLINE
DCOM- 20210708
LR  - 20210708
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 138
DP  - 2021 Sep
TI  - Effects of glycyrrhizin on the growth cycle and ATPase activity of 
      PRRSV-2-infected MARC-145 cells.
PG  - 30-38
LID - S0034-5288(21)00165-X [pii]
LID - 10.1016/j.rvsc.2021.05.011 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is a viral infectious 
      disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV) 
      and is devastating the swine industry. MARC-145 cells, an African green monkey 
      kidney cell line, are sensitive to PRRSV-2, and are often used for in vitro 
      studies on PRRSV-2. Preliminary research has shown that glycyrrhizin, an 
      important active component extracted from traditional Chinese medicinal licorice, 
      significantly inhibits the proliferation of PRRSV-2 in MARC-145 cells; however, 
      the in-depth molecular mechanism remains unclear. By determining the cell growth 
      cycle, this study found that PRRSV-2 infection first increased the content of 
      G1-phase MARC-145 cells and then decreased the content of G1-phase cells. 
      Moreover, glycyrrhizin affected the role of PRRSV-2 in regulating the cell cycle. 
      Furthermore, PRRSV-2 had the highest proliferation titer in G0/G1-phase MARC-145 
      cells, and glycyrrhizin reduced the content of PRRSV-2 in synchronized MARC-145 
      cells. According to the results of ATPase detection, PRRSV-2 infection weakened 
      the Na(+)/K(+)-ATPase and Ca(2+)/Mg(2+)-ATPase activities in MARC-145 cells, 
      while glycyrrhizin significantly enhanced their activities in PRRSV-2-infected 
      MARC-145 cells. The above results provide theoretical support toward clarifying 
      the mechanism by which glycyrrhizin inhibits the proliferation of PRRSV-2 in 
      MARC-145 cells. Moreover, these results offer references for the development and 
      use of glycyrrhizin and the clinical treatment of PRRSV-2 infection.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Duan, Erzhen
AU  - Duan E
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan 450001, China.
FAU - Zhang, Beibei
AU  - Zhang B
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan 450001, China.
FAU - Liang, Xiaoqing
AU  - Liang X
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan 450001, China.
FAU - Jing, Huiyuan
AU  - Jing H
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, Henan 450046, China.
FAU - Liu, Cen
AU  - Liu C
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan 450001, China.
FAU - Zhang, Fenghua
AU  - Zhang F
AD  - Kaifeng Center for Animal Disease Control and Prevention, Kaifeng, Henan, China.
FAU - Huang, Jin
AU  - Huang J
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan 450001, China.
FAU - Su, Lanli
AU  - Su L
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan 450001, China.
FAU - Wang, Jinrong
AU  - Wang J
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      Henan 450001, China. Electronic address: wangjr@haut.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210521
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Antiviral Agents)
RN  - 6FO62043WK (Glycyrrhizic Acid)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Adenosine Triphosphatases/*metabolism
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Glycyrrhizic Acid/*pharmacology
MH  - Kidney
MH  - Porcine Reproductive and Respiratory Syndrome/drug therapy
MH  - Porcine respiratory and reproductive syndrome virus/*drug 
      effects/enzymology/growth & development
MH  - Swine
OTO - NOTNLM
OT  - ATPase activity
OT  - Cell cycle
OT  - Glycyrrhizin
OT  - MARC-145 cell
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
EDAT- 2021/06/07 06:00
MHDA- 2021/07/09 06:00
CRDT- 2021/06/06 20:57
PHST- 2021/01/18 00:00 [received]
PHST- 2021/05/10 00:00 [revised]
PHST- 2021/05/18 00:00 [accepted]
PHST- 2021/06/07 06:00 [pubmed]
PHST- 2021/07/09 06:00 [medline]
PHST- 2021/06/06 20:57 [entrez]
AID - S0034-5288(21)00165-X [pii]
AID - 10.1016/j.rvsc.2021.05.011 [doi]
PST - ppublish
SO  - Res Vet Sci. 2021 Sep;138:30-38. doi: 10.1016/j.rvsc.2021.05.011. Epub 2021 May 
      21.