PMID- 34091701
OWN - NLM
STAT- MEDLINE
DCOM- 20210920
LR  - 20210920
IS  - 1432-072X (Electronic)
IS  - 0302-8933 (Linking)
VI  - 203
IP  - 7
DP  - 2021 Sep
TI  - Mechanism of nitrite transporter NirC in motility, biofilm formation, and 
      adhesion of avian pathogenic Escherichia coli.
PG  - 4221-4231
LID - 10.1007/s00203-021-02412-5 [doi]
AB  - The Escherichia coli (E. coli) nirC gene encodes a nitrite transporter, which 
      involved in transporting toxic nitrite (NO(2)(-)) from the environment into the 
      bacteria. Although the deletion of nirC gene could cause changes in motility, 
      adhesion in the previous study, and the virulence involved in the specified 
      mechanism for pathogenic E. coli remains to be known. In the present work, we 
      aimed to evaluate the role of NirC in a serotype O2:K1:H7 avian pathogenic 
      Escherichia coli (APEC) strain. For this purpose, we generated a NirC-deficient 
      mutant of APEC XM strain and examined its biological characteristics. The nirC 
      gene deletion mutant enhanced ability of motility, decreased in biofilm 
      formation, and it markedly reduced ability to adhere mouse brain microvascular 
      endothelial cell b.End3 cells. For understanding its mechanism, sequentially we 
      detected and found the stress regulator rpoS and its downstream genes csrA were 
      up-regulated in NirC-deficient mutant while diguanylate cyclase gene dgcT was 
      down-regulated. By high-performance liquid chromatography (HPLC) experiment, we 
      demonstrated the concentration of intracellular 3',5'-cyclic diguanosine 
      monophosphate (c-di-GMP) significantly decrease in nirC gene deletion mutant. 
      Taken data together, we may make a conclusion with a possible signal pathway 
      clue, due to NirC mutation, environmental NO(2)(-) accumulation leads to nitrite 
      stress and inactivates c-di-GMP synthesis by stimulating the stress regulator 
      RpoS, resulting in changes of biological characteristics.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Liu, Jiaqi
AU  - Liu J
AD  - Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and 
      Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product 
      Safety of Ministry of Education of China, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, China.
FAU - Zhang, Dong
AU  - Zhang D
AD  - Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and 
      Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product 
      Safety of Ministry of Education of China, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, China.
FAU - Lian, Siqi
AU  - Lian S
AD  - Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and 
      Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product 
      Safety of Ministry of Education of China, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, China.
FAU - Gu, Xuanqiang
AU  - Gu X
AD  - Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and 
      Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product 
      Safety of Ministry of Education of China, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, China.
FAU - Hou, Qianxi
AU  - Hou Q
AD  - Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and 
      Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product 
      Safety of Ministry of Education of China, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, China.
FAU - Xia, Pengpeng
AU  - Xia P
AD  - Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and 
      Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product 
      Safety of Ministry of Education of China, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, China. ppxia@yzu.edu.cn.
FAU - Zhu, Guoqiang
AU  - Zhu G
AUID- ORCID: 0000-0002-5491-0670
AD  - Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and 
      Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product 
      Safety of Ministry of Education of China, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, China. yzgqzhu@yzu.edu.cn.
LA  - eng
GR  - KYCX20_3004/Graduates Research & Practice Innovation Program of Jiangsu Province/
PT  - Journal Article
DEP - 20210605
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Anion Transport Proteins)
RN  - 0 (CsrA protein, E coli)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (NirC protein, E coli)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Animals
MH  - Anion Transport Proteins/genetics/*metabolism
MH  - Bacterial Adhesion/genetics
MH  - Biofilms
MH  - Cell Line
MH  - Escherichia coli/genetics/metabolism
MH  - Escherichia coli Infections
MH  - Escherichia coli Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - Mice
MH  - Mutation
MH  - RNA-Binding Proteins/genetics
MH  - Repressor Proteins/genetics
MH  - Virulence/genetics
OTO - NOTNLM
OT  - 3',5'-cyclic diguanosine monophosphate (c-di-GMP)
OT  - Avian pathogenic Escherichia coli (APEC)
OT  - Nitrite stress
OT  - nirC
EDAT- 2021/06/07 06:00
MHDA- 2021/09/21 06:00
CRDT- 2021/06/06 21:13
PHST- 2021/03/27 00:00 [received]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/05/25 00:00 [revised]
PHST- 2021/06/07 06:00 [pubmed]
PHST- 2021/09/21 06:00 [medline]
PHST- 2021/06/06 21:13 [entrez]
AID - 10.1007/s00203-021-02412-5 [pii]
AID - 10.1007/s00203-021-02412-5 [doi]
PST - ppublish
SO  - Arch Microbiol. 2021 Sep;203(7):4221-4231. doi: 10.1007/s00203-021-02412-5. Epub 
      2021 Jun 5.