PMID- 34091760
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20211028
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 479
IP  - 4
DP  - 2021 Oct
TI  - Correlating SS18-SSX immunohistochemistry (IHC) with SS18 fluorescent in situ 
      hybridization (FISH) in synovial sarcomas: a study of 36 cases.
PG  - 785-793
LID - 10.1007/s00428-021-03135-0 [doi]
AB  - The recently introduced, highly sensitive and specific SS18-SSX 
      immunohistochemistry (IHC) is an attractive alternative to SS18 fluorescence in 
      situ hybridization (FISH) testing in synovial sarcoma (SS). However, little is 
      known about how SS18-SSX IHC correlates with SS18 FISH. We correlated the SS18 
      FISH results of SS from 36 patients with SS18-SSX IHC. Twenty-six tumours had a 
      classic break-apart FISH pattern (1 fused, 1 red and 1 green signal) and all 
      stained positive for the IHC. Ten had an atypical (non-classic) FISH pattern of 
      which 5 stained positive for the IHC. Four of these (including two with novel 
      atypical SS18 FISH patterns) were confirmed to harbour the SS18-SSX fusion on 
      targeted RNA sequencing, while one had classic features of a biphasic SS. The 
      remaining 5 tumours stained negative for the IHC. One had a TPM3-NTRK1 fusion, 
      and one had no fusion, while the remaining three had insufficient tissue/RNA for 
      sequencing. The sensitivity of the IHC was 91% (after excluding the 2 cases with 
      confirmed absence of SS18-SSX fusion). Twenty histologic mimics of SS also 
      stained negative for the IHC (100% specificity). Our study shows that the 
      SS18-SSX IHC is more specific than SS18 FISH in diagnosing SS, especially in 
      cases with atypical FISH patterns. It correlates well with RNA sequencing result 
      and has the potential to replace SS18 FISH testing. A positive IHC result 
      supports the diagnosis of SS, while a tumour with atypical FISH pattern and 
      negative IHC result should undergo further molecular testing.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Tay, Timothy Kwang Yong
AU  - Tay TKY
AUID- ORCID: 0000-0001-8592-5018
AD  - Department of Anatomical Pathology, Singapore General Hospital, 20 College Road, 
      Academia, Diagnostic Tower Level 10, Singapore, 169856, Singapore. 
      timothy.tay.k.y@singhealth.com.sg.
AD  - Department of Molecular Pathology, Singapore General Hospital, Singapore, 
      Singapore. timothy.tay.k.y@singhealth.com.sg.
FAU - Sukma, Nadiah Binte
AU  - Sukma NB
AD  - Department of Anatomical Pathology, Singapore General Hospital, 20 College Road, 
      Academia, Diagnostic Tower Level 10, Singapore, 169856, Singapore.
FAU - Lim, Tse Hui
AU  - Lim TH
AD  - Department of Molecular Pathology, Singapore General Hospital, Singapore, 
      Singapore.
FAU - Kuick, Chik Hong
AU  - Kuick CH
AD  - Department of Pathology and Laboratory Medicine, KK Women's and Children's 
      Hospital, Singapore, Singapore.
FAU - Goh, Jian Yuan
AU  - Goh JY
AD  - Department of Pathology and Laboratory Medicine, KK Women's and Children's 
      Hospital, Singapore, Singapore.
FAU - Chang, Kenneth Tou En
AU  - Chang KTE
AD  - Department of Pathology and Laboratory Medicine, KK Women's and Children's 
      Hospital, Singapore, Singapore.
LA  - eng
PT  - Journal Article
DEP - 20210605
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SS18 protein, human)
RN  - 0 (SS18-SSX1 fusion protein)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/genetics
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Proto-Oncogene Proteins/immunology/*metabolism
MH  - Repressor Proteins/genetics/immunology/*metabolism
MH  - Sarcoma, Synovial/*genetics/immunology/metabolism
OTO - NOTNLM
OT  - FISH
OT  - Immunohistochemistry
OT  - SS18
OT  - SS18-SSX
OT  - Synovial sarcoma
EDAT- 2021/06/07 06:00
MHDA- 2021/10/29 06:00
CRDT- 2021/06/06 21:15
PHST- 2021/04/08 00:00 [received]
PHST- 2021/06/01 00:00 [accepted]
PHST- 2021/05/30 00:00 [revised]
PHST- 2021/06/07 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
PHST- 2021/06/06 21:15 [entrez]
AID - 10.1007/s00428-021-03135-0 [pii]
AID - 10.1007/s00428-021-03135-0 [doi]
PST - ppublish
SO  - Virchows Arch. 2021 Oct;479(4):785-793. doi: 10.1007/s00428-021-03135-0. Epub 
      2021 Jun 5.