PMID- 34091825
OWN - NLM
STAT- MEDLINE
DCOM- 20220207
LR  - 20220207
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Linking)
VI  - 58
IP  - 9
DP  - 2021 Sep
TI  - Circulating lncRNAs HIF1A-AS2 and LINLK-A: Role and Relation to Hypoxia-Inducible 
      Factor-1alpha in Cerebral Stroke Patients.
PG  - 4564-4574
LID - 10.1007/s12035-021-02440-8 [doi]
AB  - Long noncoding RNAs (lncRNAs) have been recently recognized as key players of 
      gene expression in cerebral pathogenesis. Thus, their potential use in stroke 
      diagnosis, prognosis, and therapy is actively pursued. Due to the complexity of 
      the disease, identifying stroke-specific lncRNAs remains a challenge. This study 
      investigated the expression of lncRNAs HIF1A-AS2 and LINK-A, and their target 
      gene hypoxia-inducible factor-1 (HIF-1) in Egyptian stroke patients. It also 
      aimed to determine the molecular mechanism implicated in the disease. A total of 
      75 stroke patients were divided into three clinical subgroups, besides 25 healthy 
      controls of age-matched and sex-matched. Remarkable upregulation of lncRNA 
      HIF1A-AS2 and HIF1-alpha along with a downregulation of lncRNA LINK-A was noticed in 
      all stroke groups relative to controls. Serum levels of phosphatidylinositol 
      3-kinase (PI3K), phosphorylated-Akt (p-Akt), vascular endothelial growth factor 
      (VEGF), and angiopoietin-1 (ANG1) as well as their receptors, malondialdehyde 
      (MDA), and total antioxidant capacity (TAC) were significantly increased, whereas 
      brain-derived neurotrophic factor (BDNF) levels were significantly decreased 
      particularly in hemorrhagic stroke versus ischemic groups. Eventually, these 
      findings support the role of lncRNAs HIF1A-AS2 and LINK-A as well as HIF1-alpha in 
      activation of angiogenesis, neovascularization, and better prognosis of stroke, 
      especially the hemorrhagic type.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Ewida, Heba A
AU  - Ewida HA
AD  - Pharmacology, Toxicology and Biochemistry Department, Faculty of Pharmaceutical 
      Sciences and Pharmaceutical Industries, Future University in Egypt (FUE), Cairo, 
      Egypt.
FAU - Zayed, Rana K
AU  - Zayed RK
AD  - Pharmacology, Toxicology and Biochemistry Department, Faculty of Pharmaceutical 
      Sciences and Pharmaceutical Industries, Future University in Egypt (FUE), Cairo, 
      Egypt.
FAU - Darwish, Hebatallah A
AU  - Darwish HA
AUID- ORCID: 0000-0001-5482-5559
AD  - Pharmacology, Toxicology and Biochemistry Department, Faculty of Pharmaceutical 
      Sciences and Pharmaceutical Industries, Future University in Egypt (FUE), Cairo, 
      Egypt. Hebatallah.darwish@pharma.cu.edu.eg.
AD  - Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt. 
      Hebatallah.darwish@pharma.cu.edu.eg.
FAU - Shaheen, Amira A
AU  - Shaheen AA
AD  - Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20210605
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - 0 (Angiopoietin-1)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Angiopoietin-1/blood
MH  - Down-Regulation
MH  - Female
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*blood
MH  - Male
MH  - Malondialdehyde/blood
MH  - Middle Aged
MH  - Oxidative Stress/*physiology
MH  - Phosphatidylinositol 3-Kinases/blood
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/blood
MH  - RNA, Long Noncoding
MH  - Stroke/*blood
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor A/blood
OTO - NOTNLM
OT  - Cerebral stroke
OT  - HIF1-alpha
OT  - Non-invasive stroke biomarkers
OT  - lncRNA HIF1A-AS2
OT  - lncRNA LINK-A
EDAT- 2021/06/07 06:00
MHDA- 2022/02/08 06:00
CRDT- 2021/06/06 21:17
PHST- 2021/03/18 00:00 [received]
PHST- 2021/05/25 00:00 [accepted]
PHST- 2021/06/07 06:00 [pubmed]
PHST- 2022/02/08 06:00 [medline]
PHST- 2021/06/06 21:17 [entrez]
AID - 10.1007/s12035-021-02440-8 [pii]
AID - 10.1007/s12035-021-02440-8 [doi]
PST - ppublish
SO  - Mol Neurobiol. 2021 Sep;58(9):4564-4574. doi: 10.1007/s12035-021-02440-8. Epub 
      2021 Jun 5.