PMID- 34092075
OWN - NLM
STAT- MEDLINE
DCOM- 20210712
LR  - 20211204
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 35
IP  - 3
DP  - 2021 May-Jun
TI  - Maintenance of cellular annexin A1 level is essential for PI3K/AKT/mTOR-mediated 
      proliferation of pancreatic beta cells.
PG  - 1011-1019
LID - 10.23812/20-417-A [doi]
AB  - Annexin A1 (AnxA1, also known as lipocortin-1), is a calcium-dependent 
      phospholipid binding protein with diverse functions. Previous studies have 
      indicated that AnxA1 is associated with age-related beta-cell dysfunction and aging, 
      which lead to decreased beta-cell proliferation capacity. However, it has been 
      uncertain whether AnxA1 affects the proliferation of pancreatic beta (beta) cells. 
      In the present study, we reduced AnxA1 expression in the MIN6 islet beta-cell line 
      using small interfering RNA (AnxA1-siRNA), then measured cell cycle distribution 
      and cellular proliferation. We also measured the expression levels of cell 
      cycle-related proteins such as cyclin D1, cyclin E, and cyclin-dependent kinase 2 
      (CDK2) by Western blot analysis. We investigated the phosphatidylinositol 
      3-kinase (PI3K)/ serine/threonine protein kinase B (Akt)/mammalian target of 
      rapamycin (mTOR) signaling pathway to explore the potential mechanism underlying 
      the observed effects. Knockdown of AnxA1 expression using siRNA reduced the rates 
      of MIN6 cell proliferation. The proportions of cells in S and G2/M phases also 
      decreased upon inhibition of AnxA1. Moreover, AnxA1 protein expression in MIN6 
      cells was positively related to the protein levels of cyclin D1, cyclin E, and 
      CDK2. Activation of the PI3K/Akt/mTOR signaling pathway by AnxA1 may be involved 
      in the signaling cascade to regulate cell proliferation. This study identified a 
      positive correlation between AnxA1 protein and pancreatic beta-cell proliferation. 
      AnxA1 protein expression might affect the proliferation of MIN6 cells via 
      regulation of cyclin D1, cyclin E, and CDK2 proteins, as well as the 
      PI3K/Akt/mTOR signaling pathway.
CI  - Copyright 2020 Biolife Sas. www.biolifesas.org.
FAU - Gu, Z Y
AU  - Gu ZY
AD  - Department of Endocrinology, Second Medical Center, Chinese PLA General Hospital, 
      Beijing, China.
AD  - National Clinical Research Center for Geriatric Diseases, Chinese PLA General 
      Hospital, Beijing, China.
FAU - Miao, X Y
AU  - Miao XY
AD  - Department of Endocrinology, Second Medical Center, Chinese PLA General Hospital, 
      Beijing, China.
AD  - National Clinical Research Center for Geriatric Diseases, Chinese PLA General 
      Hospital, Beijing, China.
FAU - Ma, L C
AU  - Ma LC
AD  - Department of Endocrinology, Second Medical Center, Chinese PLA General Hospital, 
      Beijing, China.
AD  - National Clinical Research Center for Geriatric Diseases, Chinese PLA General 
      Hospital, Beijing, China.
FAU - Gao, J J
AU  - Gao JJ
AD  - Department of Nephrology, the 306th Hospital of Chinese PLA, Beijing, China.
FAU - Gong, Y P
AU  - Gong YP
AD  - Department of Endocrinology, Second Medical Center, Chinese PLA General Hospital, 
      Beijing, China.
AD  - National Clinical Research Center for Geriatric Diseases, Chinese PLA General 
      Hospital, Beijing, China.
FAU - Li, C L
AU  - Li CL
AD  - Department of Endocrinology, Second Medical Center, Chinese PLA General Hospital, 
      Beijing, China.
AD  - National Clinical Research Center for Geriatric Diseases, Chinese PLA General 
      Hospital, Beijing, China.
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Annexin A1)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - *Annexin A1/genetics
MH  - Cell Proliferation
MH  - *Insulin-Secreting Cells/metabolism
MH  - Phosphatidylinositol 3-Kinase
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
OTO - NOTNLM
OT  - AnxA1
OT  - Islet beta cell
OT  - cell cycle
OT  - signaling pathway
EDAT- 2021/06/08 06:00
MHDA- 2021/07/13 06:00
CRDT- 2021/06/07 04:58
PHST- 2021/06/07 04:58 [entrez]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/07/13 06:00 [medline]
AID - 25 [pii]
AID - 10.23812/20-417-A [doi]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2021 May-Jun;35(3):1011-1019. doi: 
      10.23812/20-417-A.