PMID- 34092238
OWN - NLM
STAT- MEDLINE
DCOM- 20220120
LR  - 20220120
IS  - 1755-8794 (Electronic)
IS  - 1755-8794 (Linking)
VI  - 14
IP  - 1
DP  - 2021 Jun 6
TI  - Deregulation of lncRNA HIST1H2AG-6 and AIM1-3 in peripheral blood mononuclear 
      cells is associated with newly diagnosed type 2 diabetes.
PG  - 149
LID - 10.1186/s12920-021-00994-z [doi]
LID - 149
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is mainly affected by genetic and 
      environmental factors; however, the correlation of long noncoding RNAs (lncRNAs) 
      with T2DM remains largely unknown. METHODS: Microarray analysis was performed to 
      identify the differentially expressed lncRNAs and messenger RNAs (mRNAs) in 
      patients with T2DM and healthy controls, and the expression of two candidate 
      lncRNAs (lnc-HIST1H2AG-6 and lnc-AIM1-3) were further validated using 
      quantitative real-time polymerase chain reaction (qRT-PCR). Spearman's rank 
      correlation coefficient was used to measure the degree of association between the 
      two candidate lncRNAs and differentially expressed mRNAs. Furthermore, the KEGG 
      (Kyoto Encyclopedia of Genes and Genomes) pathway and GO (Gene Ontology) 
      enrichment analysis were used to reveal the biological functions of the two 
      candidate lncRNAs. Additionally, multivariate logistic regression analysis and 
      receiver operating characteristic (ROC) curve analysis were performed. RESULTS: 
      The microarray analysis revealed that there were 55 lncRNAs and 36 mRNAs 
      differentially expressed in patients with T2DM compared with healthy controls. 
      Notably, lnc-HIST1H2AG-6 was significantly upregulated and lnc-AIM1-3 was 
      significantly downregulated in patients with T2DM, which was validated in a 
      large-scale qRT-PCR examination (90 controls and 100 patients with T2DM). 
      Spearman's rank correlation coefficient revealed that both lncRNAs were 
      correlated with 36 differentially expressed mRNAs. Furthermore, functional 
      enrichment (KEGG and GO) analysis demonstrated that the two lncRNA-related mRNAs 
      might be involved in multiple biological functions, including cell programmed 
      death, negative regulation of insulin receptor signal, and starch and sucrose 
      metabolism. Multivariate logistic regression analysis revealed that 
      lnc-HIST1H2AG-6 and lnc-AIM1-3 were significantly correlated with T2DM 
      (OR = 5.791 and 0.071, respectively, both P = 0.000). Furthermore, the ROC curve 
      showed that the expression of lnc-HIST1H2AG-6 and lnc-AIM1-3 might be used to 
      differentiate patients with T2DM from healthy controls (area under the ROC 
      curve = 0.664 and 0.769, respectively). CONCLUSION: The profiles of lncRNA and 
      mRNA were significantly changed in patients with T2DM. The expression levels of 
      lnc-HIST1H2AG-6 and lnc-AIM1-3 genes were significantly correlated with some 
      features of T2DM, which may be used to distinguish patients with T2DM from 
      healthy controls and may serve as potential novel biomarkers for diagnosis in the 
      future.
FAU - Jiang, Hui
AU  - Jiang H
AD  - Department of Endocrinology, Affiliated People's Hospital of Jiangsu University, 
      Zhenjiang, 212002, China.
FAU - Lou, Peian
AU  - Lou P
AD  - Xuzhou Center for Disease Control Prevention, Xuzhou, 221000, China.
FAU - Chen, Xiaoluo
AU  - Chen X
AD  - Department of Endocrinology, Affiliated People's Hospital of Jiangsu University, 
      Zhenjiang, 212002, China.
FAU - Wu, Chenguang
AU  - Wu C
AD  - Department of Endocrinology, Affiliated People's Hospital of Jiangsu University, 
      Zhenjiang, 212002, China.
FAU - Shao, Shihe
AU  - Shao S
AD  - School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, 
      Jiangsu Province, China. shaoshihe2006@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210606
PL  - England
TA  - BMC Med Genomics
JT  - BMC medical genomics
JID - 101319628
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Leukocytes, Mononuclear
MH  - *RNA, Long Noncoding
PMC - PMC8182924
OTO - NOTNLM
OT  - Gene expression
OT  - Lnc-AIM1-3
OT  - Lnc-HIST1H2AG-6
OT  - Long noncoding RNA
OT  - Microarray analysis
OT  - Type 2 diabetes mellitus
COIS- The authors declare that they have no competing interests.
EDAT- 2021/06/08 06:00
MHDA- 2022/01/21 06:00
PMCR- 2021/06/06
CRDT- 2021/06/07 05:26
PHST- 2020/04/29 00:00 [received]
PHST- 2021/05/31 00:00 [accepted]
PHST- 2021/06/07 05:26 [entrez]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2022/01/21 06:00 [medline]
PHST- 2021/06/06 00:00 [pmc-release]
AID - 10.1186/s12920-021-00994-z [pii]
AID - 994 [pii]
AID - 10.1186/s12920-021-00994-z [doi]
PST - epublish
SO  - BMC Med Genomics. 2021 Jun 6;14(1):149. doi: 10.1186/s12920-021-00994-z.