PMID- 3409291
OWN - NLM
STAT- MEDLINE
DCOM- 19881004
LR  - 20190720
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 253
IP  - 2
DP  - 1988 Aug
TI  - Atropine inhibits the degranulation of Paneth cells in ex-germ-free mice.
PG  - 397-402
AB  - Previous studies have shown that the secretory products of Paneth cells contain 
      antibacterial agents (lysozyme, IgA) that are affected by the bacterial milieu in 
      the intestine. To investigate whether Paneth-cell secretion is controlled via 
      cholinergic mechanisms, the ultrastructure of Paneth cells was studied in four 
      animal groups: (1) germ-free (GF) control mice (Jcl: ICR [GN], male, 13 weeks 
      old), (2) GF mice injected subcutaneously with atropine sulfate (200 mg/kg body 
      weight, dissolved in physiological saline 20 mg/ml), (3) ex-GF mice inoculated 
      with feces from specific-pathogen-free (SPF) mice, and (4) ex-GF mice injected 
      with atropine and inoculated with feces from SPF mice. In ex-GF mice inoculated 
      with feces, 70-90% of the Paneth cells showed fewer secretory granules than those 
      from GF mice (p less than 0.01). Approximately 30% of the Paneth cells had a 
      large vacuole (3-10 micron diameter) in the apical cytoplasm. Exocytosed 
      electron-dense material from secretory granules was observed in a few crypt 
      lumens. In ex-GF mice inoculated with feces and given atropine, about 90% of the 
      Paneth cells contained numerous secretory granules, like those in GF control 
      mice, but vacuolated Paneth cells and exocytotic figures were rare; thus the 
      secretion of Paneth cells was blocked by atropine. It is therefore possible that 
      the bacterial milieu in the intestine affects the secretory activity of Paneth 
      cells via cholinergic mechanisms.
FAU - Satoh, Y
AU  - Satoh Y
AD  - Department of Anatomy, Asahikawa Medical College, Japan.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 7C0697DR9I (Atropine)
SB  - IM
MH  - Animals
MH  - Atropine/*pharmacology
MH  - Cytoplasmic Granules/drug effects/*metabolism/ultrastructure
MH  - Intestinal Mucosa/drug effects/*metabolism/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microscopy, Electron
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
AID - 10.1007/BF00222296 [doi]
PST - ppublish
SO  - Cell Tissue Res. 1988 Aug;253(2):397-402. doi: 10.1007/BF00222296.