PMID- 34093711
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210608
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2021
DP  - 2021
TI  - HLA DR Genome Editing with TALENs in Human iPSCs Produced Immune-Tolerant 
      Dendritic Cells.
PG  - 8873383
LID - 10.1155/2021/8873383 [doi]
LID - 8873383
AB  - Although human induced pluripotent stem cells (iPSCs) can serve as a universal 
      cell source for regenerative medicine, the use of iPSCs in clinical applications 
      is limited by prohibitive costs and prolonged generation time. Moreover, 
      allogeneic iPSC transplantation requires preclusion of mismatches between the 
      donor and recipient human leukocyte antigen (HLA). We, therefore, generated 
      universally compatible immune nonresponsive human iPSCs by gene editing. 
      Transcription activator-like effector nucleases (TALENs) were designed for 
      selective elimination of HLA DR expression. The engineered nucleases completely 
      disrupted the expression of HLA DR on human dermal fibroblast cells (HDF) that 
      did not express HLA DR even after stimulation with IFN-gamma. Teratomas formed by HLA 
      DR knockout iPSCs did not express HLA DR, and dendritic cells differentiated from 
      HLA DR knockout iPSCs reduced CD4(+) T cell activation. These engineered iPSCs 
      might provide a novel translational approach to treat multiple recipients from a 
      limited number of cell donors.
CI  - Copyright (c) 2021 Yoo-Wook Kwon et al.
FAU - Kwon, Yoo-Wook
AU  - Kwon YW
AUID- ORCID: 0000-0003-2418-429X
AD  - Strategic Center of Cell and Bio Therapy for Heart, Diabetes & Cancer, Biomedical 
      Research Institute, Seoul National University Hospital, Seoul 03080, Republic of 
      Korea.
FAU - Ahn, Hyo-Suk
AU  - Ahn HS
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of 
      Korea.
FAU - Lee, Jin-Woo
AU  - Lee JW
AD  - Strategic Center of Cell and Bio Therapy for Heart, Diabetes & Cancer, Biomedical 
      Research Institute, Seoul National University Hospital, Seoul 03080, Republic of 
      Korea.
FAU - Yang, Han-Mo
AU  - Yang HM
AD  - Cardiovascular Center & Department of Internal Medicine, Seoul National 
      University Hospital, Seoul 03080, Republic of Korea.
FAU - Cho, Hyun-Jai
AU  - Cho HJ
AD  - Cardiovascular Center & Department of Internal Medicine, Seoul National 
      University Hospital, Seoul 03080, Republic of Korea.
FAU - Kim, Seok Joong
AU  - Kim SJ
AD  - ToolGen, Inc., #1204, Byucksan Digital Valley 6-cha, 219 Gasan Digital 1-ro, 
      Geumcheon-gu, Seoul 08501, Republic of Korea.
FAU - Lee, Shin-Hyae
AU  - Lee SH
AD  - SK Chemical, Life Science Business, Clinical Research Team, Gyeonggi-do, 13494, 
      Republic of Korea.
FAU - Yang, Heung-Mo
AU  - Yang HM
AD  - GenNBio, Inc., 422, Teheran-ro, Gangnam-gu, Seoul 06193, Republic of Korea.
FAU - Jang, Hyun-Duk
AU  - Jang HD
AD  - Strategic Center of Cell and Bio Therapy for Heart, Diabetes & Cancer, Biomedical 
      Research Institute, Seoul National University Hospital, Seoul 03080, Republic of 
      Korea.
FAU - Kim, Sung Joo
AU  - Kim SJ
AD  - GenNBio, Inc., 422, Teheran-ro, Gangnam-gu, Seoul 06193, Republic of Korea.
FAU - Kim, Hyo-Soo
AU  - Kim HS
AUID- ORCID: 0000-0003-0847-5329
AD  - Strategic Center of Cell and Bio Therapy for Heart, Diabetes & Cancer, Biomedical 
      Research Institute, Seoul National University Hospital, Seoul 03080, Republic of 
      Korea.
AD  - Cardiovascular Center & Department of Internal Medicine, Seoul National 
      University Hospital, Seoul 03080, Republic of Korea.
AD  - Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence 
      Science and Technology, Seoul National University, Seoul 03080, Republic of 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20210520
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC8163544
COIS- The authors declare no conflict of interest.
EDAT- 2021/06/08 06:00
MHDA- 2021/06/08 06:01
PMCR- 2021/05/20
CRDT- 2021/06/07 05:51
PHST- 2020/08/26 00:00 [received]
PHST- 2021/03/30 00:00 [revised]
PHST- 2021/04/24 00:00 [accepted]
PHST- 2021/06/07 05:51 [entrez]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/06/08 06:01 [medline]
PHST- 2021/05/20 00:00 [pmc-release]
AID - 10.1155/2021/8873383 [doi]
PST - epublish
SO  - Stem Cells Int. 2021 May 20;2021:8873383. doi: 10.1155/2021/8873383. eCollection 
      2021.