PMID- 34093765
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210608
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 22
IP  - 2
DP  - 2021 Aug
TI  - Puerarin exhibits antiinflammatory properties in gunpowder smog-induced acute 
      lung injury in rats via regulation of the renin-angiotensin system and the NFkappaB 
      signaling pathway.
PG  - 809
LID - 10.3892/etm.2021.10241 [doi]
LID - 809
AB  - Puerarin, which is a widely used in Traditional Chinese Medicine, was previously 
      demonstrated to regulate the subsets of CD4(+) lymphocytes in gunpowder 
      smog-induced acute lung injury (ALI). However, the underlying mechanism remains 
      largely unknown. Previous studies on autoimmune diseases have revealed that the 
      renin-angiotensin system (RAS) and NF-kappaB participate in regulating the levels of 
      CD4(+) T lymphocytes. The aim of the present study was to further investigate the 
      mechanisms underlying the protective effects of puerarin. Wistar rats were 
      randomly divided into four groups as follows: Normal control, puerarin control, 
      smoke inhalation injury and puerarin treatment plus smoke inhalation injury 
      groups. The levels of angiotensin II (Ang II) in lung tissue and in the 
      circulation, and the levels of interleukin (IL)-6, IL-1beta, IL-17A and tumor 
      necrosis factor (TNF)-alpha in the bronchoalveolar lavage fluid (BALF) were assayed 
      using ELISA kits. The expression of Ang II type 1 receptor (AT1-R), 
      angiotensin-converting enzyme (ACE) and ACE2 were examined by immunohistochemical 
      analysis and western blotting. Phosphorylated (p-) NF-kappaB p65 and NF-kappaB inhibitor 
      alpha (IkappaB-alpha) protein expression levels were also determined using western blotting. 
      Puerarin treatment reduced the levels of inflammatory cytokines in the BALF. 
      Furthermore, puerarin treatment significantly decreased the levels of Ang II, 
      AT1-R and ACE, which were increased following smoke inhalation. Conversely, 
      puerarin treatment upregulated the expression of ACE2, which was downregulated 
      following smoke inhalation. Additionally, puerarin decreased the expression of 
      p-NF-kappaB p65 and increased that of IkB-alpha. Thus, the antiinflammatory effects of 
      puerarin were partly mediated via the RAS and via regulation of the NFkB 
      signaling pathway in rats with gunpowder smog-induced ALI.
CI  - Copyright: (c) Zhang et al.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Department of Clinical Laboratory Medicine, Chinese People's Liberation Army 
      General Hospital and Postgraduate Medical School, Beijing 100853, P.R. China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Department of Clinical Laboratory Medicine, Chinese People's Liberation Army 
      General Hospital and Postgraduate Medical School, Beijing 100853, P.R. China.
FAU - Liu, Peipei
AU  - Liu P
AD  - Department of Clinical Laboratory Medicine, Chinese People's Liberation Army 
      General Hospital and Postgraduate Medical School, Beijing 100853, P.R. China.
FAU - Di, Ping
AU  - Di P
AD  - Department of Clinical Laboratory Medicine, Chinese People's Liberation Army 
      General Hospital and Postgraduate Medical School, Beijing 100853, P.R. China.
FAU - Li, Mianyang
AU  - Li M
AD  - Department of Clinical Laboratory Medicine, Chinese People's Liberation Army 
      General Hospital and Postgraduate Medical School, Beijing 100853, P.R. China.
FAU - Wang, Chengbin
AU  - Wang C
AD  - Department of Clinical Laboratory Medicine, Chinese People's Liberation Army 
      General Hospital and Postgraduate Medical School, Beijing 100853, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210527
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8170659
OTO - NOTNLM
OT  - nuclear factor-kappaB
OT  - puerarin
OT  - renin-angiotensin system
OT  - smoke inhalation injury
COIS- The authors declare that they have no competing interests.
EDAT- 2021/06/08 06:00
MHDA- 2021/06/08 06:01
PMCR- 2021/05/27
CRDT- 2021/06/07 05:52
PHST- 2020/10/29 00:00 [received]
PHST- 2021/04/28 00:00 [accepted]
PHST- 2021/06/07 05:52 [entrez]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/06/08 06:01 [medline]
PHST- 2021/05/27 00:00 [pmc-release]
AID - ETM-0-0-10241 [pii]
AID - 10.3892/etm.2021.10241 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Aug;22(2):809. doi: 10.3892/etm.2021.10241. Epub 2021 May 27.