PMID- 34094670
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210608
IS  - 2156-6976 (Print)
IS  - 2156-6976 (Electronic)
IS  - 2156-6976 (Linking)
VI  - 11
IP  - 5
DP  - 2021
TI  - Laryngeal squamous cell carcinoma cell lines show high tolerance for 
      siRNA-mediated CDK1 knockdown.
PG  - 2081-2094
AB  - Alterations of the cell cycle checkpoints lead to uncontrolled cell growth and 
      result in tumorigenesis. One of the genes essential for cell proliferation and 
      cell cycle regulation is CDK1. This makes it a potential target in cancer 
      therapy. In our previous study we have shown upregulation of this gene in 
      laryngeal squamous cell carcinoma (LSCC). Here we analyze the impact of 
      siRNA-mediated CDK1 knockdown on cell proliferation and viability, measured with 
      cell growth monitoring and colorimetric test (CCK8 assay), respectively. We 
      proved that a reduction of CDK1 expression by more than 50% has no effect on 
      these cellular processes in LSCC cell lines (n=2). Moreover, using microarrays, 
      we analyzed global gene expression deregulation in these cell lines after CDK1 
      knockdown. We searched for enriched ontologies in the group of identified 137 
      differentially expressed genes (>2-fold change). Within this group we found 3 
      enriched pathways: protein binding (GO:0005515), mitotic nuclear division 
      (GO:0007067) and transmembrane receptor protein tyrosine kinase signaling pathway 
      (GO:0007169) and a group of 11 genes encoding proteins for which interaction with 
      CDK1 was indicated with the use of bioinformatic tools. Among these genes we 
      propose three: CDK6, CALD1 and FYN as potentially dependent on CDK1.
CI  - AJCR Copyright (c) 2021.
FAU - Bednarek, Kinga
AU  - Bednarek K
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
FAU - Kostrzewska-Poczekaj, Magdalena
AU  - Kostrzewska-Poczekaj M
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
FAU - Ustaszewski, Adam
AU  - Ustaszewski A
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
FAU - Janiszewska, Joanna
AU  - Janiszewska J
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
FAU - Kiwerska, Katarzyna
AU  - Kiwerska K
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
AD  - Department of Tumor Pathology, Greater Poland Cancer Centre Poznan, Poland.
FAU - Paczkowska, Julia
AU  - Paczkowska J
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
FAU - Grenman, Reidar
AU  - Grenman R
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Turku University 
      Central Hospital and Turku University Turku, Finland.
FAU - Giefing, Maciej
AU  - Giefing M
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
FAU - Jarmuz-Szymczak, Malgorzata
AU  - Jarmuz-Szymczak M
AD  - Institute of Human Genetics, Polish Academy of Sciences Poznan, Poland.
AD  - Department of Hematology and Bone Marrow Transplantation, Poznan University of 
      Medical Sciences Poznan, Poland.
LA  - eng
PT  - Journal Article
DEP - 20210515
PL  - United States
TA  - Am J Cancer Res
JT  - American journal of cancer research
JID - 101549944
PMC - PMC8167681
OTO - NOTNLM
OT  - CDK1
OT  - cell cycle checkpoint
OT  - gene silencing
OT  - laryngeal squamous cell carcinoma (LSCC)
COIS- None.
EDAT- 2021/06/08 06:00
MHDA- 2021/06/08 06:01
PMCR- 2021/05/15
CRDT- 2021/06/07 06:00
PHST- 2021/01/12 00:00 [received]
PHST- 2021/03/24 00:00 [accepted]
PHST- 2021/06/07 06:00 [entrez]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/06/08 06:01 [medline]
PHST- 2021/05/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Cancer Res. 2021 May 15;11(5):2081-2094. eCollection 2021.