PMID- 34094899
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240402
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - Iron Regulates the Warburg Effect and Ferroptosis in Colorectal Cancer.
PG  - 614778
LID - 10.3389/fonc.2021.614778 [doi]
LID - 614778
AB  - Iron promotes the proliferation of cancer cells, but it also contributes to cell 
      death. Here we explored whether iron could promote the Warburg effect of 
      colorectal cancer (CRC) cells and suppress sensitivity to ferroptosis by inducing 
      reactive oxygen species (ROS) and regulating nuclear factor erythroid 2-related 
      factor 2 (NRF2). In this study, cell proliferation abilities were measured by 
      CCK-8, EdU incorporation, and colony formation assays. Seahorse XF96 respirometry 
      assays were used to detect the Warburg effect and the level of ROS was assess by 
      DCFH-DA fluorescent probes. Results showed that iron exposure promoted the 
      Warburg effect of CRC cells by inducing ROS and activating NRF2 both in vivo and 
      in vitro. In addition, iron exposure also induced ferroptosis in CRC cells, but 
      at the same time its inhibitory proteins SLC7A11 and GPX4 were also upregulated, 
      indicating an enhanced resistance to ferroptosis. Our results revealed that iron 
      can effectively promote tumorigenesis. Meanwhile, iron elimination or a low-iron 
      diet might be valid therapeutic approaches for CRC.
CI  - Copyright (c) 2021 Yuan, Ni, Zhuge, Li and Li.
FAU - Yuan, Yin
AU  - Yuan Y
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 
      China.
FAU - Ni, Shuo
AU  - Ni S
AD  - Department of Orthopedics, Shanghai Pudong Hospital, Fudan University Pudong 
      Medical Center, Shanghai, China.
FAU - Zhuge, Aoxiang
AU  - Zhuge A
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 
      China.
FAU - Li, Bo
AU  - Li B
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 
      China.
AD  - Research Units of Infectious Disease and Microecology, Chinese Academy of Medical 
      Sciences, Hangzhou, China.
FAU - Li, Lanjuan
AU  - Li L
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The 
      First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 
      China.
AD  - Research Units of Infectious Disease and Microecology, Chinese Academy of Medical 
      Sciences, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210518
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8169994
OTO - NOTNLM
OT  - NRF2
OT  - ROS
OT  - ferroptosis
OT  - iron
OT  - the Warburg effect
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/06/08 06:00
MHDA- 2021/06/08 06:01
PMCR- 2021/01/01
CRDT- 2021/06/07 06:03
PHST- 2020/10/07 00:00 [received]
PHST- 2021/04/12 00:00 [accepted]
PHST- 2021/06/07 06:03 [entrez]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/06/08 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.614778 [doi]
PST - epublish
SO  - Front Oncol. 2021 May 18;11:614778. doi: 10.3389/fonc.2021.614778. eCollection 
      2021.