PMID- 34096020
OWN - NLM
STAT- MEDLINE
DCOM- 20210608
LR  - 20210608
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 38
IP  - 6
DP  - 2021 Jun 10
TI  - [Differential expression and bioinformatic analysis of microRNA in the plasma of 
      patients with type 2 diabetes mellitus].
PG  - 536-540
LID - 10.3760/cma.j.cn511374-20200103-00006 [doi]
AB  - OBJECTIVE: To construct the differential expression profile of microRNA (miRNA) 
      in plasma of patients with type 2 diabetes mellitus (T2DM) and explore the 
      possibility of using miRNA as the target for diagnosis and treatment of T2DM. 
      METHODS: Agilent miRNA microarray was used to determine the expression profiles 
      of miRNA in the plasma of patients with T2DM (FC> 2, P< 0.05). The result was 
      verified by real-time quantitative PCR (RT-qPCR). Candidate miRNA was analyzed by 
      bioinformatic tools. RESULTS: In total 122 differentially expressed miRNAs were 
      identified. Among these, 14 were selected by multi-source intersection screening, 
      which included 5 up-regulated genes and 9 down regulated genes. RT-qPCR showed 
      that the expression of hsa-miR-185-5p and hsa-miR-328-5p have significantly 
      increased in T2DM patients (P< 0.05). Bioinformatic analysis suggested that these 
      miRNAs may be involved in the pathogenesis of T2DM through insulin secretion and 
      PI3K-AKT signaling pathway. CONCLUSION: Differential expression of hsa-miR-185-5p 
      and hsa-miR-328-5p in the plasma may be closely associated with the pathogenesis 
      of T2DM.
FAU - Ye, Ying
AU  - Ye Y
AD  - Fujian Provincial Center for Disease Control, Fuzhou, Fujian 350001, China. 
      yeying78@163.com.
FAU - Liu, Xiaohua
AU  - Liu X
FAU - Yan, Yansheng
AU  - Yan Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Computational Biology
MH  - *Diabetes Mellitus, Type 2/genetics
MH  - Humans
MH  - *MicroRNAs/genetics
MH  - Phosphatidylinositol 3-Kinases
MH  - Signal Transduction
EDAT- 2021/06/08 06:00
MHDA- 2021/06/09 06:00
CRDT- 2021/06/07 06:19
PHST- 2021/06/07 06:19 [entrez]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/06/09 06:00 [medline]
AID - 940638113 [pii]
AID - 10.3760/cma.j.cn511374-20200103-00006 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2021 Jun 10;38(6):536-540. doi: 
      10.3760/cma.j.cn511374-20200103-00006.