PMID- 34096152
OWN - NLM
STAT- MEDLINE
DCOM- 20211103
LR  - 20211103
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Linking)
VI  - 60
IP  - 34
DP  - 2021 Aug 16
TI  - Enzyme Mimic Facilitated Artificial Cell to Mammalian Cell Signal Transfer.
PG  - 18704-18711
LID - 10.1002/anie.202104904 [doi]
AB  - Catalyzing biochemical reactions with enzymes and communicating with neighboring 
      cells via chemical signaling are two fundamental cellular features that play a 
      critical role in maintaining the homeostasis of organisms. Herein, we present an 
      artificial enzyme (AE) facilitated signal transfer between artificial cells (ACs) 
      and mammalian HepG2 cells. We synthesize metalloporphyrins (MPs) based AEs that 
      mimic cytochrome P450 enzymes (CYPs) to catalyze a dealkylation and a 
      hydroxylation reaction, exemplified by the conversion of resorufin ethyl ether 
      (REE) to resorufin and coumarin (COU) to 7-hydroxycoumarin (7-HC), respectively. 
      The AEs are immobilized in hydrogels to produce ACs that generate the two 
      diffusive fluorophores, which can diffuse into HepG2 cells and result in dual 
      intracellular emissions. This work highlights the use of AEs to promote AC to 
      mammalian signal transfer, which opens up new opportunities for integrating the 
      synthetic and living world with a bottom-up strategy.
CI  - (c) 2021 Wiley-VCH GmbH.
FAU - Qian, Xiaomin
AU  - Qian X
AUID- ORCID: 0000-0002-2598-0661
AD  - Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 
      14, 8000, Aarhus, Denmark.
FAU - Westensee, Isabella Nymann
AU  - Westensee IN
AUID- ORCID: 0000-0003-4895-0964
AD  - Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 
      14, 8000, Aarhus, Denmark.
FAU - Fernandes, Catarina Cavalheiro
AU  - Fernandes CC
AD  - Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 
      14, 8000, Aarhus, Denmark.
FAU - Stadler, Brigitte
AU  - Stadler B
AUID- ORCID: 0000-0002-7335-3945
AD  - Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 
      14, 8000, Aarhus, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210716
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 0 (Coumarins)
RN  - 0 (Oxazines)
RN  - 635-78-9 (resorufin)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - A4VZ22K1WT (coumarin)
SB  - IM
MH  - Artificial Cells/chemistry/*metabolism
MH  - Biocatalysis
MH  - Coumarins/chemistry/metabolism
MH  - Cytochrome P-450 Enzyme System/metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Oxazines/chemistry/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - artificial cell
OT  - artificial enzyme
OT  - cytochrome P450 mimic
OT  - metalloporphyrins
OT  - signal transfer
EDAT- 2021/06/08 06:00
MHDA- 2021/11/04 06:00
CRDT- 2021/06/07 06:29
PHST- 2021/05/25 00:00 [revised]
PHST- 2021/04/09 00:00 [received]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/11/04 06:00 [medline]
PHST- 2021/06/07 06:29 [entrez]
AID - 10.1002/anie.202104904 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2021 Aug 16;60(34):18704-18711. doi: 
      10.1002/anie.202104904. Epub 2021 Jul 16.