PMID- 34098454
OWN - NLM
STAT- MEDLINE
DCOM- 20211216
LR  - 20211216
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 88
DP  - 2021 Aug
TI  - Long term neuroprotective effects of acute single dose MK-801treatment against 
      traumatic brain injury in immature rats.
PG  - 102161
LID - S0143-4179(21)00047-0 [pii]
LID - 10.1016/j.npep.2021.102161 [doi]
AB  - Because brain development continues during adolescence, childhood trauma is a 
      major health problem in pediatric ages. It is known traumatic brain injury (TBI) 
      results in damage in hippocampal and cortical areas of the brain and impairs 
      cognitive functions. The study aims to investigate the long-term effects of 
      MK-801 (dizocilpine), an N-methyl d-aspartate (NMDA) receptor antagonist, on 
      hippocampal damage, locomotor activity, and cognitive functions following TBI in 
      immature rats. MK-801 (1 mg/kg) was injected intraperitoneally immediately after 
      TBI. Thirty-seven litters were randomly allocated into three groups at 7 days 
      (P7) of postnatal age: a control group, a trauma group, and an MK-801 treatment 
      group. The control group received no treatment; the trauma group received saline 
      as vehicle control for the MK-801 group and the MK-801 group received a single 
      dose of 1 mg/kg MK-801 immediately after TBI. Hippocampal damage was examined by 
      Hematoxylin-Eosin staining. Brain-derived neurotrophic factor (BDNF), nerve 
      growth factor (NGF), NMDA-R, and glial fibrillar acidic protein (GFAP) 
      immunohistochemistry and, BDNF, NGF, and NMDA-R ELISA protein levels were 
      evaluated 125 days after trauma. Histopathological and immunohistochemical 
      evaluations showed that treatment with MK-801 significantly ameliorated the 
      trauma-induced hippocampal neuron loss and increased BDNF, NGF, NMDA-R, GFAP 
      expressions in CA1, CA3, and DG hippocampal regions. Additionally, treatment with 
      MK-801 decreased anxiety and increased hippocampus-dependent memory of animals 
      subjected to brain injury after TBI. These results show that acute treatment of 
      MK-801 has a neuroprotective role against trauma-induced hippocampal neuron loss 
      and associated cognitive impairment in rats.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Cigel, Ayse
AU  - Cigel A
AD  - Department of Physiology, Faculty of Medicine, Dokuz Eylul University, Izmir, 
      Turkey. Electronic address: acigel@adu.edu.tr.
FAU - Sayin, Oya
AU  - Sayin O
AD  - Research Laboratory, Faculty of Medicine Dokuz Eylul University, Izmir, Turkey. 
      Electronic address: oya.sayin@deu.edu.tr.
FAU - Gurgen, Seren Gulsen
AU  - Gurgen SG
AD  - Department of Histology and Embryology, Faculty of Medicine, Celal Bayar 
      University, Manisa, Turkey.
FAU - Sonmez, Atac
AU  - Sonmez A
AD  - Department of Physiology, Faculty of Medicine, Dokuz Eylul University, Izmir, 
      Turkey. Electronic address: atac.sonmez@deu.edu.tr.
LA  - eng
PT  - Journal Article
DEP - 20210521
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Neuroprotective Agents)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/metabolism
MH  - Brain Injuries, Traumatic/*drug therapy/metabolism
MH  - Dizocilpine Maleate/*pharmacology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Hippocampus/drug effects/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Rats
MH  - *Time
OTO - NOTNLM
OT  - BDNF
OT  - Cognition
OT  - Hippocampus
OT  - MK-801
OT  - NGF
OT  - NMDA-R
OT  - Traumatic brain injury
EDAT- 2021/06/08 06:00
MHDA- 2021/12/17 06:00
CRDT- 2021/06/07 20:28
PHST- 2021/01/04 00:00 [received]
PHST- 2021/04/28 00:00 [revised]
PHST- 2021/05/16 00:00 [accepted]
PHST- 2021/06/08 06:00 [pubmed]
PHST- 2021/12/17 06:00 [medline]
PHST- 2021/06/07 20:28 [entrez]
AID - S0143-4179(21)00047-0 [pii]
AID - 10.1016/j.npep.2021.102161 [doi]
PST - ppublish
SO  - Neuropeptides. 2021 Aug;88:102161. doi: 10.1016/j.npep.2021.102161. Epub 2021 May 
      21.