PMID- 34099588
OWN - NLM
STAT- MEDLINE
DCOM- 20211209
LR  - 20211214
IS  - 1899-1505 (Electronic)
IS  - 0867-5910 (Linking)
VI  - 72
IP  - 1
DP  - 2021 Feb
TI  - Altered serum levels of autophagy proteins Beclin-1 and mTOR in patients with 
      exudative age-related macular degeneration.
LID - 10.26402/jpp.2021.1.09 [doi]
AB  - Autophagy is a key process in the maintenance of cellular survival and 
      homeostasis. Inhibition of autophagy results in degenerative changes resembling 
      ageing. We wondered if autophagy can contribute to the pathogenesis of 
      age-related macular degeneration (AMD). We aimed to investigate the serum 
      concentrations of two key autophagy regulators, Beclin-1 and mechanistic target 
      of rapamycin (mTOR), in patients with exudative AMD. This retrospective 
      case-control study included 38 patients with exudative AMD and 36 sex- and 
      age-matched controls selected among senile cataract patients. Circulating 
      Beclin-1 and mTOR were assessed using an enzyme-linked immunosorbent assay. The 
      proteins levels were correlated with age, sex, duration of ocular symptoms, as 
      well as angiographic and optical coherence tomography findings. Serum Beclin-1 
      levels were much lower in patients with AMD than in controls (median, 0.100 ng/ml 
      versus 1.123 ng/ml; p = 0.0033), while mTOR levels did not differ (median, 4.377 
      ng/ml versus 3.608 ng/ml; p = 0.4522). Participants of the study older than 70 
      years had lower Beclin-1 levels than younger ones (p = 0.0444). However, this 
      difference was the most evident in patients with AMD (p = 0.0024). Serum mTOR 
      levels increased with age. In patients with AMD, lower mTOR levels were 
      associated with drusen, while higher levels were observed in those with a fibrous 
      scar in the contralateral eye (p = 0.0212). Our findings suggest that circulating 
      Beclin-1 decreases with age and that is downregulated in patients with AMD.
FAU - Kubicka-Trzaska, A
AU  - Kubicka-Trzaska A
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Ophthalmology, Cracow, Poland. agnieszka.kubicka-trzaska@uj.edu.pl.
FAU - Zuber-Laskawiec, K
AU  - Zuber-Laskawiec K
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Ophthalmology, Cracow, Poland.
FAU - Plutecka, H
AU  - Plutecka H
AD  - Jagiellonian University Medical College, Faculty of Medicine, Division of 
      Molecular Biology and Clinical Genetics, Cracow, Poland.
FAU - Romanowska-Dixon, B
AU  - Romanowska-Dixon B
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Ophthalmology, Cracow, Poland.
FAU - Sanak, M
AU  - Sanak M
AD  - Jagiellonian University Medical College, Faculty of Medicine, Division of 
      Molecular Biology and Clinical Genetics, Cracow, Poland.
FAU - Karska-Basta, I
AU  - Karska-Basta I
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Ophthalmology, Cracow, Poland.
LA  - eng
PT  - Journal Article
DEP - 20210221
PL  - Poland
TA  - J Physiol Pharmacol
JT  - Journal of physiology and pharmacology : an official journal of the Polish 
      Physiological Society
JID - 9114501
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Age Factors
MH  - Aged
MH  - Aged, 80 and over
MH  - Autophagy/*physiology
MH  - Beclin-1/*blood
MH  - Case-Control Studies
MH  - Female
MH  - Fluorescein Angiography
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - TOR Serine-Threonine Kinases/*blood
MH  - Tomography, Optical Coherence
MH  - Wet Macular Degeneration/blood/*physiopathology
EDAT- 2021/06/09 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/06/08 05:58
PHST- 2021/01/29 00:00 [received]
PHST- 2021/02/26 00:00 [accepted]
PHST- 2021/06/08 05:58 [entrez]
PHST- 2021/06/09 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
AID - 10.26402/jpp.2021.1.09 [doi]
PST - ppublish
SO  - J Physiol Pharmacol. 2021 Feb;72(1). doi: 10.26402/jpp.2021.1.09. Epub 2021 Feb 
      21.