PMID- 34099590
OWN - NLM
STAT- MEDLINE
DCOM- 20210721
LR  - 20210721
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 13
IP  - 11
DP  - 2021 Jun 7
TI  - Myeloid-specific SIRT1 deletion exacerbates airway inflammatory response in a 
      mouse model of allergic asthma.
PG  - 15479-15490
LID - 10.18632/aging.203104 [doi]
AB  - Sirtuin 1 (SIRT1) is a class III histone deacetylase that exerts an 
      anti-inflammatory effect in airway diseases. Activated macrophages play an 
      important role in asthma. However, the roles of SIRT1 on allergic airway 
      inflammation in macrophages remain largely unexplored. In this study, we aimed to 
      determine the roles of SIRT1 on allergic airway inflammation in macrophages. The 
      effect of myeloid-specific SIRT1 deletion (Sirt1(fl/fl)-LysMcre) on airway 
      inflammation was assessed by using in vivo models of asthma following allergen 
      exposure and in vitro culture of primary bone marrow-derived macrophages (BMDMs) 
      exposed to house dust mite (HDM). We observed that Sirt1(fl/fl)-LysMcre mice 
      substantially enhanced airway inflammation and mucus production in response to 
      allergen exposure. Expression of chemokine ligand (CXCL) 2, interleukin (IL)-1beta, 
      and tumor necrosis factor (TNF)-alpha were reduced in BMDMs with myeloid-specific 
      deletion of Sirt1 after stimulation of HDM. Moreover, SIRT1 suppressed the 
      inflammatory cytokines expression in BMDMs partially via the ERK/p38 MAPK 
      pathways. Our study demonstrated that SIRT1 suppresses the allergic airway 
      inflammation in macrophages, and suggested that activation of SIRT1 in 
      macrophages may represent therapeutic strategy for asthma.
FAU - Lai, Tianwen
AU  - Lai T
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Su, Guomei
AU  - Su G
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Wu, Dong
AU  - Wu D
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Chen, Ziyu
AU  - Chen Z
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Chen, Yujuan
AU  - Chen Y
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Yi, Huajuan
AU  - Yi H
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Gao, Yun
AU  - Gao Y
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Chen, Cuifen
AU  - Chen C
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Zeng, Man
AU  - Zeng M
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Chen, Min
AU  - Chen M
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Li, Dongming
AU  - Li D
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
FAU - Wu, Bin
AU  - Wu B
AD  - Department of Respiratory and Critical Care Medicine, Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524001, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210607
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Allergens/adverse effects
MH  - Animals
MH  - Asthma/complications/*pathology
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - *Gene Deletion
MH  - Hypersensitivity/complications/*pathology
MH  - Inflammation/complications/*pathology
MH  - Integrases/metabolism
MH  - Lung/*pathology
MH  - MAP Kinase Signaling System
MH  - Macrophages/metabolism/pathology
MH  - Mice, Transgenic
MH  - Myeloid Cells/*metabolism
MH  - Pyroglyphidae
MH  - Sirtuin 1/*metabolism
PMC - PMC8221322
OTO - NOTNLM
OT  - BMDM
OT  - SIRT1
OT  - asthma
OT  - macrophage
COIS- CONFLICTS OF INTEREST: The authors declare that they have no conflicts of 
      interest.
EDAT- 2021/06/09 06:00
MHDA- 2021/07/22 06:00
PMCR- 2021/06/15
CRDT- 2021/06/08 05:58
PHST- 2020/12/27 00:00 [received]
PHST- 2021/05/13 00:00 [accepted]
PHST- 2021/06/09 06:00 [pubmed]
PHST- 2021/07/22 06:00 [medline]
PHST- 2021/06/08 05:58 [entrez]
PHST- 2021/06/15 00:00 [pmc-release]
AID - 203104 [pii]
AID - 10.18632/aging.203104 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2021 Jun 7;13(11):15479-15490. doi: 10.18632/aging.203104. 
      Epub 2021 Jun 7.