PMID- 34099759
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20240226
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jun 7
TI  - An initial assessment of the involvement of transglutaminase2 in eosinophilic 
      bronchitis using a disease model developed in C57BL/6 mice.
PG  - 11946
LID - 10.1038/s41598-021-90950-9 [doi]
LID - 11946
AB  - The detailed pathogenesis of eosinophilic bronchitis (EB) remains unclear. 
      Transglutaminase 2 (TG2) has been implicated in many respiratory diseases 
      including asthma. Herein, we aim to assess preliminarily the relationship of TG2 
      with EB in the context of the development of an appropriate EB model through 
      ovalbumin (OVA) sensitization and challenge in the C57BL/6 mouse strain. Our data 
      lead us to propose a 50 mug dose of OVA challenge as appropriate to establish an 
      EB model in C57BL/6 mice, whereas a challenge with a 400 mug dose of OVA 
      significantly induced asthma. Compared to controls, TG2 is up-regulated in the 
      airway epithelium of EB mice and EB patients. When TG2 activity was inhibited by 
      cystamine treatment, there were no effects on airway responsiveness; in contrast, 
      the lung pathology score and eosinophil counts in bronchoalveolar lavage fluid 
      were significantly increased whereas the cough frequency was significantly 
      decreased. The expression levels of interleukin (IL)-4, IL-13, IL-6, mast cell 
      protease7 and the transient receptor potential (TRP) ankyrin 1 (TRPA1), TRP 
      vanilloid 1 (TRPV1) were significantly decreased. These data open the possibility 
      of an involvement of TG2 in mediating the increased cough frequency in EB through 
      the regulation of TRPA1 and TRPV1 expression. The establishment of an EB model in 
      C57BL/6 mice opens the way for a genetic investigation of the involvement of TG2 
      and other molecules in this disease using KO mice, which are often generated in 
      the C57BL/6 genetic background.
FAU - Chen, Lan
AU  - Chen L
AD  - Sino-French Hoffmann Institute, Guangzhou, China.
FAU - Liu, Shuyan
AU  - Liu S
AD  - Sino-French Hoffmann Institute, Guangzhou, China.
FAU - Xiao, Linzhuo
AU  - Xiao L
AD  - Sino-French Hoffmann Institute, Guangzhou, China.
FAU - Chen, Kanyao
AU  - Chen K
AD  - Sino-French Hoffmann Institute, Guangzhou, China.
FAU - Tang, Juanjuan
AU  - Tang J
AD  - Sino-French Hoffmann Institute, Guangzhou, China.
FAU - Huang, Chuqin
AU  - Huang C
AD  - State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory 
      Health, Guangzhou Medical University, Guangzhou, China.
FAU - Luo, Wei
AU  - Luo W
AD  - State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory 
      Health, Guangzhou Medical University, Guangzhou, China.
FAU - Ferrandon, Dominique
AU  - Ferrandon D
AD  - Sino-French Hoffmann Institute, Guangzhou, China.
AD  - State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory 
      Health, Guangzhou Medical University, Guangzhou, China.
AD  - Universite de Strasbourg, M3I UPR9022 du CNRS, 67000, Strasbourg, France.
FAU - Lai, Kefang
AU  - Lai K
AD  - State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory 
      Health, Guangzhou Medical University, Guangzhou, China. klai@163.com.
FAU - Li, Zi
AU  - Li Z
AD  - Sino-French Hoffmann Institute, Guangzhou, China. lizi1002@gzhmu.edu.cn.
AD  - State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory 
      Health, Guangzhou Medical University, Guangzhou, China. lizi1002@gzhmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210607
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cytokines)
RN  - 0 (TRPA1 Cation Channel)
RN  - 0 (Trpa1 protein, mouse)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 2.3.2.13 (Protein Glutamine gamma Glutamyltransferase 2)
RN  - EC 2.3.2.13 (Transglutaminases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - R110LV8L02 (Cystamine)
SB  - IM
MH  - Animals
MH  - Asthma/chemically induced/immunology
MH  - Bronchitis/chemically induced/*immunology/metabolism
MH  - Cystamine/pharmacology
MH  - Cytokines/genetics/immunology/metabolism
MH  - *Disease Models, Animal
MH  - Eosinophils/drug effects/*immunology/metabolism
MH  - GTP-Binding Proteins/genetics/*immunology/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Inflammation/genetics/immunology/metabolism
MH  - Lung/drug effects/immunology/pathology
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Ovalbumin
MH  - Protein Glutamine gamma Glutamyltransferase 2
MH  - TRPA1 Cation Channel/genetics/immunology/metabolism
MH  - Transglutaminases/genetics/*immunology/metabolism
MH  - Mice
PMC - PMC8184915
COIS- The authors declare no competing interests.
EDAT- 2021/06/09 06:00
MHDA- 2021/11/09 06:00
PMCR- 2021/06/07
CRDT- 2021/06/08 06:22
PHST- 2020/10/14 00:00 [received]
PHST- 2021/05/12 00:00 [accepted]
PHST- 2021/06/08 06:22 [entrez]
PHST- 2021/06/09 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/06/07 00:00 [pmc-release]
AID - 10.1038/s41598-021-90950-9 [pii]
AID - 90950 [pii]
AID - 10.1038/s41598-021-90950-9 [doi]
PST - epublish
SO  - Sci Rep. 2021 Jun 7;11(1):11946. doi: 10.1038/s41598-021-90950-9.