PMID- 34103282
OWN - NLM
STAT- MEDLINE
DCOM- 20210624
LR  - 20210624
IS  - 2146-3077 (Electronic)
IS  - 1300-6320 (Linking)
VI  - 45
IP  - 2
DP  - 2021 Jun 7
TI  - Expression of Hexokinase in the Proteome Profile of Leishmania major and 
      Crithidia.
PG  - 83-87
LID - 10.4274/tpd.galenos.2020.6983 [doi]
AB  - OBJECTIVE: The relationship between drug resistance and the expression of 
      hexokinase (HK) has been indicated in leishmaniasis. According to the prolonged 
      treatment period in cutaneous leishmaniasis (CL) patients co-infected with 
      Crithidia in Iran, this study aims to investigate the expression of HK in the 
      proteome of Leishmania major and Crithidia using a proteomic approach. METHODS: A 
      total of 205 samples were removed from the lesions of patients in Fars province, 
      Iran, for the characterization of L. major and Crithidia using polymerase chain 
      reaction (PCR). After protein extraction, two-dimensional gel electrophoresis was 
      employed for protein separation. Several spots were isolated for HK determination 
      in the proteomes of L. major and Crithidia using matrix-assisted laser 
      desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF MS). 
      RESULTS: The PCR results showed 5 positive cases for Crithidia and 96 positive 
      cases for L. major. MALDI TOF/TOF MS indicated HK as a common protein in the 
      proteome of L. major and Crithidia. HK was up-regulated in the Crithidia proteome 
      in comparison with the L. major proteome. CONCLUSION: Since a relationship 
      between HK expression and drug resistance has been indicated in leishmaniasis, 
      the overexpression of HK in Crithidia might be related to the increased duration 
      of the treatment period in CL patients co-infected with Crithidia.
FAU - Karimazar, Mohammadreza
AU  - Karimazar M
AUID- ORCID: 0000-0002-2088-3950
AD  - Shiraz University of Medical Sciences Faculty of Medicine, Department of 
      Parasitology and Mycology, Shiraz, Iran
FAU - Asgari, Qasem
AU  - Asgari Q
AUID- ORCID: 0000-0002-7945-2370
AD  - Shiraz University of Medical Sciences Faculty of Medicine, Department of 
      Parasitology and Mycology, Shiraz, Iran
FAU - Rashidi, Sajad
AU  - Rashidi S
AUID- ORCID: 0000-0002-4675-7274
AD  - Shiraz University of Medical Sciences Faculty of Medicine, Department of 
      Parasitology and Mycology, Shiraz, Iran
FAU - Tavirani, Mostafa Rezaei
AU  - Tavirani MR
AUID- ORCID: 0000-0003-1767-7475
AD  - Shahid Beheshti University of Medical Sciences, Proteomics Research Center, 
      Tehran, Iran
FAU - Motazedian, Mohammad Hossein
AU  - Motazedian MH
AUID- ORCID: 0000-0003-0117-9666
AD  - Shiraz University of Medical Sciences Faculty of Medicine, Department of 
      Parasitology and Mycology, Shiraz, Iran
LA  - eng
PT  - Journal Article
TT  - Leishmania'nin Proteom Profilinde Hekzokinaz Ifadesi.
PL  - Turkey
TA  - Turkiye Parazitol Derg
JT  - Turkiye parazitolojii dergisi
JID - 9425544
RN  - 0 (Proteome)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Coinfection/drug therapy/parasitology
MH  - Crithidia/enzymology/isolation & purification/*metabolism
MH  - Drug Resistance
MH  - Euglenozoa Infections/drug therapy/parasitology
MH  - Hexokinase/*metabolism
MH  - Humans
MH  - Iran
MH  - Leishmania major/enzymology/isolation & purification/*metabolism
MH  - Proteome/*metabolism
MH  - Proteomics
OTO - NOTNLM
OT  - Crithidia
OT  - Leishmania major
OT  - proteomics
OT  - hexokinase
OT  - drug resistance
EDAT- 2021/06/10 06:00
MHDA- 2021/06/25 06:00
CRDT- 2021/06/09 05:51
PHST- 2021/06/09 05:51 [entrez]
PHST- 2021/06/10 06:00 [pubmed]
PHST- 2021/06/25 06:00 [medline]
AID - 10.4274/tpd.galenos.2020.6983 [doi]
PST - ppublish
SO  - Turkiye Parazitol Derg. 2021 Jun 7;45(2):83-87. doi: 
      10.4274/tpd.galenos.2020.6983.