PMID- 34108567
OWN - NLM
STAT- MEDLINE
DCOM- 20211102
LR  - 20240402
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jun 9
TI  - Large-scale comparative analysis of cytogenetic markers across Lepidoptera.
PG  - 12214
LID - 10.1038/s41598-021-91665-7 [doi]
LID - 12214
AB  - Fluorescence in situ hybridization (FISH) allows identification of particular 
      chromosomes and their rearrangements. Using FISH with signal enhancement via 
      antibody amplification and enzymatically catalysed reporter deposition, we 
      evaluated applicability of universal cytogenetic markers, namely 18S and 5S rDNA 
      genes, U1 and U2 snRNA genes, and histone H3 genes, in the study of the karyotype 
      evolution in moths and butterflies. Major rDNA underwent rather erratic 
      evolution, which does not always reflect chromosomal changes. In contrast, the 
      hybridization pattern of histone H3 genes was well conserved, reflecting the 
      stable organisation of lepidopteran genomes. Unlike 5S rDNA and U1 and U2 snRNA 
      genes which we failed to detect, except for 5S rDNA in a few representatives of 
      early diverging lepidopteran lineages. To explain the negative FISH results, we 
      used quantitative PCR and Southern hybridization to estimate the copy number and 
      organization of the studied genes in selected species. The results suggested that 
      their detection was hampered by long spacers between the genes and/or their 
      scattered distribution. Our results question homology of 5S rDNA and U1 and U2 
      snRNA loci in comparative studies. We recommend the use of histone H3 in studies 
      of karyotype evolution.
FAU - Provaznikova, Irena
AU  - Provaznikova I
AUID- ORCID: 0000-0003-2336-458X
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
AD  - European Molecular Biology Laboratory, Heidelberg, Germany.
FAU - Hejnickova, Martina
AU  - Hejnickova M
AUID- ORCID: 0000-0002-9303-305X
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
FAU - Visser, Sander
AU  - Visser S
AUID- ORCID: 0000-0002-0953-0871
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
AD  - Groningen Institute for Evolutionary Life Sciences, University of Groningen, 
      Groningen, The Netherlands.
FAU - Dalikova, Martina
AU  - Dalikova M
AUID- ORCID: 0000-0002-0266-8894
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
FAU - Carabajal Paladino, Leonela Z
AU  - Carabajal Paladino LZ
AUID- ORCID: 0000-0001-9312-0260
AD  - The Pirbright Institute, Surrey, UK.
FAU - Zrzava, Magda
AU  - Zrzava M
AUID- ORCID: 0000-0001-8482-9614
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
FAU - Volenikova, Anna
AU  - Volenikova A
AUID- ORCID: 0000-0002-9504-8044
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
FAU - Marec, Frantisek
AU  - Marec F
AUID- ORCID: 0000-0002-6745-5603
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
FAU - Nguyen, Petr
AU  - Nguyen P
AUID- ORCID: 0000-0003-1395-4287
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic. petr.nguyen@prf.jcu.cz.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic. 
      petr.nguyen@prf.jcu.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210609
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (U2 small nuclear RNA)
SB  - IM
MH  - Animals
MH  - Butterflies/*genetics
MH  - Chromosome Mapping
MH  - Cytogenetic Analysis/methods
MH  - DNA, Ribosomal/*genetics
MH  - *Evolution, Molecular
MH  - Genome
MH  - In Situ Hybridization, Fluorescence
MH  - Moths/*genetics
MH  - RNA, Ribosomal, 18S/*genetics
MH  - RNA, Ribosomal, 5S/*genetics
MH  - RNA, Small Nuclear/*genetics
PMC - PMC8190105
COIS- The authors declare no competing interests.
EDAT- 2021/06/11 06:00
MHDA- 2021/11/03 06:00
PMCR- 2021/06/09
CRDT- 2021/06/10 06:26
PHST- 2021/03/26 00:00 [received]
PHST- 2021/05/24 00:00 [accepted]
PHST- 2021/06/10 06:26 [entrez]
PHST- 2021/06/11 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/06/09 00:00 [pmc-release]
AID - 10.1038/s41598-021-91665-7 [pii]
AID - 91665 [pii]
AID - 10.1038/s41598-021-91665-7 [doi]
PST - epublish
SO  - Sci Rep. 2021 Jun 9;11(1):12214. doi: 10.1038/s41598-021-91665-7.