PMID- 34110415 OWN - NLM STAT- MEDLINE DCOM- 20220228 LR - 20220929 IS - 1758-535X (Electronic) IS - 1079-5006 (Print) IS - 1079-5006 (Linking) VI - 76 IP - 11 DP - 2021 Oct 13 TI - Interbatch Reliability of Blood-Based Cytokine and Chemokine Measurements in Community-Dwelling Older Adults: A Cross-Sectional Study. PG - 1954-1961 LID - 10.1093/gerona/glab162 [doi] AB - Blood-based inflammatory markers hold considerable promise for diagnosis and prognostication of age-related neurodegenerative disease, though a paucity of research has empirically tested how reliably they can be measured across different experimental runs ("batches"). We quantified the interbatch reliability of 13 cytokines and chemokines in a cross-sectional study of 92 community-dwelling older adults (mean age = 74; 48% female). Plasma aliquots from the same blood draw were parallelly processed in 2 separate batches using the same analytic platform and procedures (high-performance electrochemiluminescence by Meso Scale Discovery). Interbatch correlations (Pearson's r) ranged from small and nonsignificant (r = .13 for macrophage inflammatory protein-1 alpha [MIP-1alpha]) to very large (r > .90 for interferon gamma [IFNgamma], interleukin-10 [IL-10], interferon gamma-induced protein 10 [IP-10], MIP-1beta, thymus and activation-regulated chemokine [TARC]) with most markers falling somewhere in between (.67