PMID- 34113509
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210612
IS  - 2168-8184 (Print)
IS  - 2168-8184 (Electronic)
IS  - 2168-8184 (Linking)
VI  - 13
IP  - 5
DP  - 2021 May 7
TI  - Study of Single Nucleotide Polymorphisms in Endosomal Toll-Like Receptors-3, 7, 
      and 9 Genes in Patients With Dengue: A Case-Control Study.
PG  - e14883
LID - 10.7759/cureus.14883 [doi]
LID - e14883
AB  - Introduction The endosomal toll-like receptors (TLR) TLR3, TLR7, and TLR9 are 
      localized in immune cells, recognize the viral pattern associated molecular 
      pattern (PAMPs), and start signaling cascades for immune defense response and 
      genetic factor is known to affect the dengue virus infection therefore in our 
      study we study the association of endosomal Toll-like receptors 3 polymorphism 
      rs3775291, rs3775290, and rs3775296 with rs179008 A/T and rs179009C/T 
      polymorphisms of TLR7 and rs187084 (C/T), rs5743836 (C/T) of TLR9 gene with 
      dengue and controls. Materials and methods Ninety-eight cases of dengue virus 
      (DV) infection and 98 healthy controls were enrolled. Clinical details were 
      recorded and cases were classified as severe or non-severe dengue, based on WHO 
      2009 classification. Genotypes were determined by Sanger sequencing using the 
      genetic analyzer. Results An increased risk of DV infection was observed in cases 
      as compared to controls, with TLR 3 rs3775291 CT genotype (OR = 4.34, P-value: 
      0.031, CI = 1.14-16.5), Likewise in TLR7 rs179008 AT (OR = 2.12, P-value: 0.034, 
      CI = 1.06-4.26) and rs179009 CT (OR = 2.04, P-value: 0.040, CI = 1.03-4.05) same 
      as in TLR9 rs187084 CT (OR = 1.97, P-value: 0.046, CI = 1.013-3.84) and rs5743836 
      (OR = 2.38, P-value: 0.009, CI = 1.24-5.57). In the above polymorphisms, mutant 
      allele was observed in a significantly higher number in cases. The values are: 
      for TLR3 rs3775291 T allele (OR 2.167, CI = 1.3-3.61, P-value: 0.003). TLR7 
      rs179008 T allele (OR 1.90, P-value: 0.021, CI = 1.07-3.35) and in TLR9 rs187084 
      (OR 1.91, P-value: 0.014, CI = 1.137-3.24) rs5743836 (OR 2.29, P-value: 0.0018, 
      CI = 1.36-3.87). No significant association was observed in the genotypic 
      frequency of severe versus non-severe dengue. Conclusion TLR3, 7, and 9 gene 
      polymorphism might confer host genetic susceptibility to dengue in the North 
      Indian population.
CI  - Copyright (c) 2021, Singh et al.
FAU - Singh, Arvind K
AU  - Singh AK
AD  - Department of Microbiology, King George's Medical University, Lucknow, IND.
FAU - Prakash, Shantanu
AU  - Prakash S
AD  - Department of Microbiology, King George's Medical University, Lucknow, IND.
FAU - Garg, Ravindra Kumar
AU  - Garg RK
AD  - Department of Neurology, King George's Medical University, Lucknow, IND.
FAU - Jain, Parul
AU  - Jain P
AD  - Department of Microbiology, King George's Medical University, Lucknow, IND.
FAU - Kumar, Rashmi
AU  - Kumar R
AD  - Department of Pediatrics, King George's Medical University, Lucknow, IND.
FAU - Jain, Amita
AU  - Jain A
AD  - Department of Microbiology, King George's Medical University, Lucknow, IND.
LA  - eng
PT  - Journal Article
DEP - 20210507
PL  - United States
TA  - Cureus
JT  - Cureus
JID - 101596737
PMC - PMC8184108
OTO - NOTNLM
OT  - dengue
OT  - innate immunity
OT  - tlr 9
OT  - tlr3
OT  - tlr7
OT  - toll-like receptors
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/06/12 06:00
MHDA- 2021/06/12 06:01
PMCR- 2021/05/07
CRDT- 2021/06/11 06:45
PHST- 2021/06/11 06:45 [entrez]
PHST- 2021/06/12 06:00 [pubmed]
PHST- 2021/06/12 06:01 [medline]
PHST- 2021/05/07 00:00 [pmc-release]
AID - 10.7759/cureus.14883 [doi]
PST - epublish
SO  - Cureus. 2021 May 7;13(5):e14883. doi: 10.7759/cureus.14883.