PMID- 34113558
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210612
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - Gene Expression Profile of the Human Colorectal Carcinoma LoVo Cells Treated With 
      Sporamin and Thapsigargin.
PG  - 621462
LID - 10.3389/fonc.2021.621462 [doi]
LID - 621462
AB  - Sporamin, a proteinase inhibitor isolated from the sweet potato (Ipomoea 
      batatas), has shown promising anticancer effect against colorectal cancer (CRC) 
      in vitro and in vivo but its mechanisms of action are poorly understood. In the 
      present study, high throughput RNA sequencing (RNA-seq) technology was applied to 
      explore the transcriptomic changes induced by sporamin in the presence of 
      thapsigargin (TG), a non-12-O-tetradecanolphorbol-13-acetate type cancer 
      promoter, in the LoVo human CRC cells. Cellular total RNA was extracted from the 
      cells after they were treated with vehicle (CTL), 1 muM of thapsigargin (TG), or 1 
      muM of TG plus 30 muM of sporamin (TGSP) for 24 h. The migratory capacity of the 
      cells was determined by wound healing assay. The gene expression profiles of the 
      cells were determined by RNA-seq on an Illumina platform. GO enrichment analysis, 
      KEGG pathway analysis, protein-protein interaction (PPI) network construction, 
      and transcription factors (TF) prediction were all performed based on the 
      differentially expressed genes (DEGs) across groups with a series of 
      bioinformatics tools. Finally, the effect and potential molecular targets of the 
      sporamin at the transcriptome level were evaluated. Sporamin significantly 
      inhibited the migration of cells induced by TG. Among the 17915 genes detected in 
      RNA-seq, 46 DEGs were attributable to the effect of sporamin. RT-PCR experiment 
      validated that the expression of RGPD2, SULT1A3, and BIVM-ERCC5 were up-regulated 
      while NYP4R, FOXN1, PAK6, and CEACAM20 were down-regulated. Sporamin enhanced the 
      mineral absorption pathway, worm longevity regulating pathway, and pyrimidine 
      metabolism pathway. Two TFs (SMIM11A and ATOH8) were down-regulated by sporamin. 
      HMOX1 (up-regulated) and NME1-NME2 (down-regulated) were the main nodes in a PPI 
      network consisting of 16 DEGs that were modulated by sporamin in the presence of 
      TG. Sporamin could favorably alter the gene expression profile of CRC cells, 
      up-regulating the genes that contribute to the homeostasis of intracellular metal 
      ions and the activities of essential enzymes and DNA damage repairment. More 
      studies are warranted to verify its effect on specific genes and delineate the 
      mechanism of action implicated in the process.
CI  - Copyright (c) 2021 Yang, Chen, Chen, Zhang, Zhang, Xiao and Li.
FAU - Yang, Chun
AU  - Yang C
AD  - School of Public Health, Capital Medical University (CMU), Beijing, China.
AD  - Beijing Key Laboratory of Environmental Toxicology, CMU, Beijing, China.
AD  - Beijing Key Laboratory of Clinical Epidemiology, CMU, Beijing, China.
FAU - Chen, Si-Jia
AU  - Chen SJ
AD  - School of Public Health, Capital Medical University (CMU), Beijing, China.
AD  - Beijing Key Laboratory of Environmental Toxicology, CMU, Beijing, China.
AD  - Beijing Key Laboratory of Clinical Epidemiology, CMU, Beijing, China.
FAU - Chen, Bo-Wen
AU  - Chen BW
AD  - School of Public Health, Capital Medical University (CMU), Beijing, China.
AD  - Beijing Key Laboratory of Environmental Toxicology, CMU, Beijing, China.
AD  - Beijing Key Laboratory of Clinical Epidemiology, CMU, Beijing, China.
FAU - Zhang, Kai-Wen
AU  - Zhang KW
AD  - School of Public Health, Capital Medical University (CMU), Beijing, China.
AD  - Beijing Key Laboratory of Environmental Toxicology, CMU, Beijing, China.
AD  - Beijing Key Laboratory of Clinical Epidemiology, CMU, Beijing, China.
FAU - Zhang, Jing-Jie
AU  - Zhang JJ
AD  - School of Public Health, Capital Medical University (CMU), Beijing, China.
AD  - Beijing Key Laboratory of Environmental Toxicology, CMU, Beijing, China.
AD  - Beijing Key Laboratory of Clinical Epidemiology, CMU, Beijing, China.
AD  - National Center for Child Nutriment Quality Supervision and Testing, China 
      National Children's Center, Beijing, China.
FAU - Xiao, Rong
AU  - Xiao R
AD  - School of Public Health, Capital Medical University (CMU), Beijing, China.
AD  - Beijing Key Laboratory of Environmental Toxicology, CMU, Beijing, China.
AD  - Beijing Key Laboratory of Clinical Epidemiology, CMU, Beijing, China.
FAU - Li, Peng-Gao
AU  - Li PG
AD  - School of Public Health, Capital Medical University (CMU), Beijing, China.
AD  - Beijing Key Laboratory of Environmental Toxicology, CMU, Beijing, China.
AD  - Beijing Key Laboratory of Clinical Epidemiology, CMU, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20210525
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8185278
OTO - NOTNLM
OT  - RNA sequencing
OT  - colorectal cancer
OT  - gene expression profile
OT  - sporamin
OT  - thapsigargin
OT  - transcriptome
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/06/12 06:00
MHDA- 2021/06/12 06:01
PMCR- 2021/01/01
CRDT- 2021/06/11 06:46
PHST- 2020/10/26 00:00 [received]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/06/11 06:46 [entrez]
PHST- 2021/06/12 06:00 [pubmed]
PHST- 2021/06/12 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.621462 [doi]
PST - epublish
SO  - Front Oncol. 2021 May 25;11:621462. doi: 10.3389/fonc.2021.621462. eCollection 
      2021.