PMID- 34113796
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220423
IS  - 2574-0954 (Electronic)
IS  - 2574-0954 (Linking)
VI  - 10
IP  - 2
DP  - 2021 Jun
TI  - Analogous telomeres shortening and different metabolic profile: hypertension 
      versus hypertension/type 2 diabetes mellitus comorbidity.
PG  - 106-112
LID - 10.1097/XCE.0000000000000232 [doi]
AB  - BACKGROUND: Eukaryotes chromosomal ends are capped and protected by telomeres, 
      which are noncoding DNA repeats synthesized by telomerase enzyme. The telomerase 
      enzyme is a nucleoprotein encoded by TERC and TERT genes. Naturally, the length 
      of the telomeres shortens with each cell cycle but the shortening is fastened in 
      certain age-related diseases like hypertension (HTN) and type 2 diabetes mellitus 
      (T2DM). MATERIALS AND METHODS: Blood samples (n = 171) were obtained from Kuwaiti 
      subjects with HTN, and HTN/T2DM comorbidity (HTN-DM) and healthy subjects. The 
      leukocyte telomere length (LTL) was measured by SYBR green quantitative rtPCR, 
      and plasma telomerase enzyme was measured by ELISA, in addition, three single 
      nucleotide polymorphisms (SNPs) in telomere-related genes; TERC rs12696304GC, 
      TERT rs2736100CA, and ACYP2 rs6713088GC were genotyped by real-time PCR. RESULTS: 
      Marked LTL shortening in subjects with HTN and HTN-DM compared to healthy 
      subjects, P = 0.043 and P < 0.001, respectively, was noticed. On the contrary, 
      the plasma telomerase enzyme levels and minor allele frequencies and genotypes of 
      the tested SNPs were comparable between the study groups, except for TERT (CA) 
      genotype which was over-represented in HTN (P = 0.037). Furthermore, the 
      comparisons between HTN and HTN-DM revealed significantly higher total 
      cholesterol (P = 0.015) and LDL-C (P = 0.008) in HTN, while higher insulin levels 
      (P < 001), HOMA-IR (P < 001), and BMI (P = 0.004) were observed in HTN-DM. 
      CONCLUSION: This study showed comparable LTL shortening in HTN and HTN-DM, 
      irrespective of plasma telomerase enzyme levels or tested TERC, TERT, and ACYP2 
      gene polymorphisms, although HTN and HTN-DM differed in several metabolic 
      markers. More studies are required to affirm these observations.
CI  - Copyright (c) 2020 Wolters Kluwer Health, Inc. All rights reserved.
FAU - AlDehaini, Dhuha M B
AU  - AlDehaini DMB
AD  - Department of Biochemistry, College of Medicine and Medical Sciences, Arabian 
      Gulf University.
AD  - Clinical Chemistry Laboratory, Salmaniya Medical Complex Hospital, Manama, 
      Kingdom of Bahrain.
FAU - Al-Bustan, Suzanne A
AU  - Al-Bustan SA
AD  - Department of Molecular Biology, Kuwait University, Kuwait City, Kuwait.
FAU - Malalla, Zainab Hasan Abdulla
AU  - Malalla ZHA
AD  - Department of Biochemistry, College of Medicine and Medical Sciences, Arabian 
      Gulf University.
FAU - Ali, Muhalab E
AU  - Ali ME
AD  - Department of Biochemistry, College of Medicine and Medical Sciences, Arabian 
      Gulf University.
FAU - Sater, Mai
AU  - Sater M
AD  - Department of Biochemistry, College of Medicine and Medical Sciences, Arabian 
      Gulf University.
FAU - Giha, Hayder A
AU  - Giha HA
AD  - Department of Biochemistry, College of Medicine and Medical Sciences, Arabian 
      Gulf University.
LA  - eng
PT  - Journal Article
DEP - 20200903
PL  - United States
TA  - Cardiovasc Endocrinol Metab
JT  - Cardiovascular endocrinology & metabolism
JID - 101730894
PMC - PMC8186511
OTO - NOTNLM
OT  - Kuwait
OT  - biochemical profile
OT  - hypertension
OT  - single nucleotide polymorphism
OT  - telomerase
OT  - telomeres
OT  - type 2 diabetes mellitus
COIS- There are no conflicts of interest.
EDAT- 2021/06/12 06:00
MHDA- 2021/06/12 06:01
PMCR- 2020/09/03
CRDT- 2021/06/11 06:50
PHST- 2020/05/04 00:00 [received]
PHST- 2020/07/27 00:00 [accepted]
PHST- 2021/06/11 06:50 [entrez]
PHST- 2021/06/12 06:00 [pubmed]
PHST- 2021/06/12 06:01 [medline]
PHST- 2020/09/03 00:00 [pmc-release]
AID - 10.1097/XCE.0000000000000232 [doi]
PST - epublish
SO  - Cardiovasc Endocrinol Metab. 2020 Sep 3;10(2):106-112. doi: 
      10.1097/XCE.0000000000000232. eCollection 2021 Jun.