PMID- 34116237
OWN - NLM
STAT- MEDLINE
DCOM- 20221215
LR  - 20221230
IS  - 2213-2961 (Electronic)
IS  - 2095-2546 (Print)
IS  - 2213-2961 (Linking)
VI  - 11
IP  - 6
DP  - 2022 Nov
TI  - Exercise attenuates angiotensinⅡ-induced muscle atrophy by targeting 
      PPARgamma/miR-29b.
PG  - 696-707
LID - S2095-2546(21)00067-3 [pii]
LID - 10.1016/j.jshs.2021.06.002 [doi]
AB  - BACKGROUND: Exercise is beneficial for muscle atrophy. Peroxisome 
      proliferator-activated receptor gamma (PPARgamma) and microRNA-29b (miR-29b) have 
      been reported to be responsible for angiotensinⅡ (AngⅡ)-induced muscle atrophy. 
      However, it is unclear whether exercise can protect AngⅡ-induced muscle atrophy 
      by targeting PPARgamma/miR-29b. METHODS: Skeletal muscle atrophy in both the control 
      group and the run group was established by AngⅡ infusion; after 1 week of 
      exercise training, the mice were sacrificed, and muscle weight was determined. 
      Myofiber size was measured by hematoxylin-eosin and wheat-germ agglutinin 
      staining. Apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP 
      nick end labeling staining. The expression level of muscle atrogenes, including 
      F-box only protein 32 (FBXO32, also called Atrogin-1) and muscle-specific 
      RING-finger 1 (MuRF-1), the phosphorylation level of protein kinase B (PKB, also 
      called AKT)/forkhead box O3A (FOXO3A)/mammalian target of rapamycin (mTOR) 
      pathway proteins, the expression level of PPARgamma and apoptosis-related proteins, 
      including B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), cysteine-aspartic 
      acid protease 3 (caspase-3), and cleaved-caspase-3, were determined by western 
      blot. The expression level of miR-29b was checked by reverse-transcription 
      quantitative polymerase chain reaction. A PPARgamma inhibitor (T0070907) or 
      adeno-associated virus serotype-8 (AAV8)-mediated miR-29b overexpression was used 
      to demonstrate whether PPARgamma activation or miR-29b inhibition mediates the 
      beneficial effects of exercise in AngⅡ-induced muscle atrophy. RESULTS: Exercise 
      can significantly attenuate AngⅡ-induced muscle atrophy, which is demonstrated by 
      increased skeletal muscle weight, cross-sectional area of myofiber, and 
      activation of AKT/mTOR signaling and by decreased atrogenes expressions and 
      apoptosis. In AngⅡ-induced muscle atrophy mice models, PPARgamma was elevated whereas 
      miR-29b was decreased by exercise. The protective effects of exercise in 
      AngⅡ-induced muscle atrophy were inhibited by a PPARgamma inhibitor (T0070907) or 
      adeno-associated virus serotype-8 (AAV8)-mediated miR-29b overexpression. 
      CONCLUSION: Exercise attenuates AngⅡ-induced muscle atrophy by activation of 
      PPARgamma and suppression of miR-29b.
CI  - Copyright (c) 2021. Production and hosting by Elsevier B.V.
FAU - Liu, Qi
AU  - Liu Q
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China.
FAU - Chen, Liyang
AU  - Chen L
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China.
FAU - Liang, Xuchun
AU  - Liang X
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China.
FAU - Cao, Yuqing
AU  - Cao Y
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China.
FAU - Zhu, Xinyue
AU  - Zhu X
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China.
FAU - Wang, Siqi
AU  - Wang S
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China.
FAU - Li, Jin
AU  - Li J
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China.
FAU - Gao, Juan
AU  - Gao J
AD  - Shanghai Engineering Research Center of Organ Repair, School of Medicine, 
      Shanghai University, Shanghai 200444, China. Electronic address: 
      juangao@shu.edu.cn.
FAU - Xiao, Junjie
AU  - Xiao J
AD  - Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School 
      of Life Science, Shanghai University, Shanghai 200444, China; Shanghai 
      Engineering Research Center of Organ Repair, School of Medicine, Shanghai 
      University, Shanghai 200444, China. Electronic address: junjiexiao@shu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210608
PL  - China
TA  - J Sport Health Sci
JT  - Journal of sport and health science
JID - 101606001
RN  - 0 (PPAR gamma)
RN  - EC 3.4.22.- (Caspase 3)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *PPAR gamma
MH  - Caspase 3
MH  - Muscular Atrophy/prevention & control
MH  - *MicroRNAs
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - Mammals
PMC - PMC9729927
OTO - NOTNLM
OT  - Angiotensin Ⅱ
OT  - Exercise
OT  - Muscle atrophy
OT  - PPARgamma
OT  - miR-29b
EDAT- 2021/06/12 06:00
MHDA- 2022/12/15 06:00
PMCR- 2021/06/08
CRDT- 2021/06/11 20:15
PHST- 2021/02/17 00:00 [received]
PHST- 2021/04/11 00:00 [revised]
PHST- 2021/05/07 00:00 [accepted]
PHST- 2021/06/12 06:00 [pubmed]
PHST- 2022/12/15 06:00 [medline]
PHST- 2021/06/11 20:15 [entrez]
PHST- 2021/06/08 00:00 [pmc-release]
AID - S2095-2546(21)00067-3 [pii]
AID - 10.1016/j.jshs.2021.06.002 [doi]
PST - ppublish
SO  - J Sport Health Sci. 2022 Nov;11(6):696-707. doi: 10.1016/j.jshs.2021.06.002. Epub 
      2021 Jun 8.