PMID- 34117611
OWN - NLM
STAT- MEDLINE
DCOM- 20211227
LR  - 20211227
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 34
IP  - 5
DP  - 2021 Sep
TI  - Suppressive role of microRNA-130b-3p in ferroptosis in melanoma cells correlates 
      with DKK1 inhibition and Nrf2-HO-1 pathway activation.
PG  - 1532-1544
LID - 10.1007/s13577-021-00557-5 [doi]
AB  - Cell death pathways related to ferroptosis are implicated in the progression of 
      melanoma. Emerging data reporting the upregulation of microRNA (miR)-130b-3p in 
      melanoma indicate the potential implication of miR-130b-3p in this malignancy. 
      Herein, we aimed to identify whether and how miR-130b-3p regulated ferroptosis in 
      melanoma cells. Melanoma cells (A375, G-361) were treated with erastin or RSL3 to 
      mimic ferroptosis in vitro. Viability, lipid peroxidation level and ferrous ion 
      content in melanoma cells were then assessed in response to manipulation of 
      miR-130b-3p expression. Luciferase assay was conducted to determine the binding 
      of miR-130b-3p to Dickkopf1 (DKK1). Western blot assay was conducted to determine 
      the expression of molecules related to nuclear factor-erythroid 2 p45-related 
      factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. The results indicated that 
      miR-130b-3p exerted an inhibitory role in erastin or RSL3-induced ferroptosis, 
      evidenced by reductions in lipid peroxidation and ferrous ion content. By 
      suppressing the expression of target gene DKK1, miR-130b-3p activated the 
      Nrf2/HO-1 pathway, whereby repressing ferroptosis. miR-130b-3p blocked the 
      antitumor activity of erastin. Further, in vitro findings were reproduced in an 
      in vivo murine model. Together, these data suggest the potential of miR-130b-3p 
      to inhibit ferroptosis in melanoma cells and the mechanism was related to 
      DKK1-mediated Nrf2/HO-1 pathway.
CI  - (c) 2021. Japan Human Cell Society.
FAU - Liao, Yangying
AU  - Liao Y
AD  - Department of Dermatology, Hunan Provincial People's HospitalThe First Affiliated 
      Hospital of Hunan Normal University)Hunan Province, No. 61, Jiefang West Road, 
      Changsha, 410005, People's Republic of China.
FAU - Jia, Xiaomin
AU  - Jia X
AD  - Department of Pathology, Lhasa People's Hospital of Tibet Autonomous Region, 
      Lhasa, 850000, People's Republic of China.
FAU - Ren, Yi
AU  - Ren Y
AD  - Beijing Jishuitan Hospital, Beijing, 100035, People's Republic of China.
FAU - Deji, Zhuoga
AU  - Deji Z
AD  - Department of Pathology, Lhasa People's Hospital of Tibet Autonomous Region, 
      Lhasa, 850000, People's Republic of China.
FAU - Gesang, Yuzhen
AU  - Gesang Y
AD  - Department of Pathology, Lhasa People's Hospital of Tibet Autonomous Region, 
      Lhasa, 850000, People's Republic of China.
FAU - Ning, Ning
AU  - Ning N
AD  - Medical Department, Hunan Provincial People's Hospital (The First Affiliated 
      Hospital of Hunan Normal University), Changsha, 410005, People's Republic of 
      China.
FAU - Feng, Hao
AU  - Feng H
AD  - Department of Dermatology, Hunan Provincial People's HospitalThe First Affiliated 
      Hospital of Hunan Normal University)Hunan Province, No. 61, Jiefang West Road, 
      Changsha, 410005, People's Republic of China. doctorfenghao@hunnu.edu.cn.
FAU - Yu, Hong
AU  - Yu H
AD  - Department of Pathology, The Third People's Hospital of Shenzhen, Shenzhen, 
      518100, People's Republic of China.
FAU - Wei, An
AU  - Wei A
AD  - Department of Ultrasound, Hunan Provincial People's Hospital (The First 
      Affiliated Hospital of Hunan Normal University), No. 61, Jiefang West Road, 
      Changsha, 410005, Hunan Province, People's Republic of China. 
      linshiwei06@163.com.
LA  - eng
GR  - 20C1125/Hunan Provincial Education Department Project/
GR  - BSJJ202008/Doctor's Fund Project of Hunan Provincial People's Hospital/
GR  - No kq2014202/Changsha Municipal Science and Technology Bureau Natural Science 
      Foundation Project/
PT  - Journal Article
DEP - 20210611
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (DKK1 protein, human)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Cell Line, Tumor
MH  - Ferroptosis/*genetics
MH  - Heme Oxygenase-1/*genetics/*metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*genetics/*metabolism
MH  - Melanoma/*genetics/*pathology
MH  - MicroRNAs/*genetics/*physiology
MH  - NF-E2-Related Factor 2/*genetics/*metabolism
MH  - Signal Transduction/*genetics/physiology
OTO - NOTNLM
OT  - DKK1
OT  - Ferroptosis
OT  - Iron accumulation
OT  - Lipid peroxidation
OT  - Melanoma
OT  - Nrf2/HO-1 pathway
OT  - microRNA-130b
EDAT- 2021/06/13 06:00
MHDA- 2021/12/28 06:00
CRDT- 2021/06/12 06:19
PHST- 2021/02/01 00:00 [received]
PHST- 2021/05/24 00:00 [accepted]
PHST- 2021/06/13 06:00 [pubmed]
PHST- 2021/12/28 06:00 [medline]
PHST- 2021/06/12 06:19 [entrez]
AID - 10.1007/s13577-021-00557-5 [pii]
AID - 10.1007/s13577-021-00557-5 [doi]
PST - ppublish
SO  - Hum Cell. 2021 Sep;34(5):1532-1544. doi: 10.1007/s13577-021-00557-5. Epub 2021 
      Jun 11.